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Increased expression of system large amino acid transporter (LAT)-1 mRNA is associated with invasive potential and unfavorable prognosis of human clear cell renal cell carcinoma

BACKGROUND: The system L amino acid transporter (LAT) has an important role in the transport of various amino acids, and there have been reports about the relation of this system to cancer. Although LATs are highly expressed in the kidneys, little is known about their influence on human renal cancer...

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Autores principales: Betsunoh, Hironori, Fukuda, Takehiko, Anzai, Naohiko, Nishihara, Daisaku, Mizuno, Tomoya, Yuki, Hideo, Masuda, Akinori, Yamaguchi, Yoshiyuki, Abe, Hideyuki, Yashi, Masahiro, Fukabori, Yoshitatsu, Yoshida, Ken-Ichiro, Kamai, Takao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3832879/
https://www.ncbi.nlm.nih.gov/pubmed/24168110
http://dx.doi.org/10.1186/1471-2407-13-509
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author Betsunoh, Hironori
Fukuda, Takehiko
Anzai, Naohiko
Nishihara, Daisaku
Mizuno, Tomoya
Yuki, Hideo
Masuda, Akinori
Yamaguchi, Yoshiyuki
Abe, Hideyuki
Yashi, Masahiro
Fukabori, Yoshitatsu
Yoshida, Ken-Ichiro
Kamai, Takao
author_facet Betsunoh, Hironori
Fukuda, Takehiko
Anzai, Naohiko
Nishihara, Daisaku
Mizuno, Tomoya
Yuki, Hideo
Masuda, Akinori
Yamaguchi, Yoshiyuki
Abe, Hideyuki
Yashi, Masahiro
Fukabori, Yoshitatsu
Yoshida, Ken-Ichiro
Kamai, Takao
author_sort Betsunoh, Hironori
collection PubMed
description BACKGROUND: The system L amino acid transporter (LAT) has an important role in the transport of various amino acids, and there have been reports about the relation of this system to cancer. Although LATs are highly expressed in the kidneys, little is known about their influence on human renal cancer. METHODS: To clarify the role of LATs in human clear cell renal cell carcinoma (RCC), we investigated the expression of mRNAs for LAT1, LAT2, LAT3, LAT4, and 4F2hc in clear cell RCC tissues. The mRNAs of these five genes were analyzed by the real-time reverse transcription polymerase chain reaction in matched sets of tumor and non-tumor tissues obtained at operation from 82 Japanese patients with clear cell RCC. We also measured phosphorylated S6 ribosomal protein (Ser-235/236) proteins levels in 18 paired tumor and non-tumor tissues of the patients by Western blotting. RESULTS: Expression of LAT1 mRNA was significantly increased in tumor tissue compared with non-tumor tissue, while expression of LAT2 and LAT3 mRNAs was reduced. There was no difference in the expression of LAT4 and 4F2hc mRNAs between tumor and non-tumor tissues. Increased expression of LAT1 mRNA was associated with less differentiated tumors, local invasion, microscopic vascular invasion, and metastasis. Kaplan-Meier survival analysis showed that a higher serum LAT1 mRNA level was associated with a shorter overall survival time. Phosphorylated S6 ribosomal protein levels were associated with metastatic potential. LAT1 mRNA levels positively correlated with phosphorylated S6 ribosomal protein proteins levels in primary tumors. CONCLUSIONS: These findings suggest that LAT1 mRNA is related to the invasive and progressive potential of clear cell RCC.
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spelling pubmed-38328792013-11-20 Increased expression of system large amino acid transporter (LAT)-1 mRNA is associated with invasive potential and unfavorable prognosis of human clear cell renal cell carcinoma Betsunoh, Hironori Fukuda, Takehiko Anzai, Naohiko Nishihara, Daisaku Mizuno, Tomoya Yuki, Hideo Masuda, Akinori Yamaguchi, Yoshiyuki Abe, Hideyuki Yashi, Masahiro Fukabori, Yoshitatsu Yoshida, Ken-Ichiro Kamai, Takao BMC Cancer Research Article BACKGROUND: The system L amino acid transporter (LAT) has an important role in the transport of various amino acids, and there have been reports about the relation of this system to cancer. Although LATs are highly expressed in the kidneys, little is known about their influence on human renal cancer. METHODS: To clarify the role of LATs in human clear cell renal cell carcinoma (RCC), we investigated the expression of mRNAs for LAT1, LAT2, LAT3, LAT4, and 4F2hc in clear cell RCC tissues. The mRNAs of these five genes were analyzed by the real-time reverse transcription polymerase chain reaction in matched sets of tumor and non-tumor tissues obtained at operation from 82 Japanese patients with clear cell RCC. We also measured phosphorylated S6 ribosomal protein (Ser-235/236) proteins levels in 18 paired tumor and non-tumor tissues of the patients by Western blotting. RESULTS: Expression of LAT1 mRNA was significantly increased in tumor tissue compared with non-tumor tissue, while expression of LAT2 and LAT3 mRNAs was reduced. There was no difference in the expression of LAT4 and 4F2hc mRNAs between tumor and non-tumor tissues. Increased expression of LAT1 mRNA was associated with less differentiated tumors, local invasion, microscopic vascular invasion, and metastasis. Kaplan-Meier survival analysis showed that a higher serum LAT1 mRNA level was associated with a shorter overall survival time. Phosphorylated S6 ribosomal protein levels were associated with metastatic potential. LAT1 mRNA levels positively correlated with phosphorylated S6 ribosomal protein proteins levels in primary tumors. CONCLUSIONS: These findings suggest that LAT1 mRNA is related to the invasive and progressive potential of clear cell RCC. BioMed Central 2013-10-30 /pmc/articles/PMC3832879/ /pubmed/24168110 http://dx.doi.org/10.1186/1471-2407-13-509 Text en Copyright © 2013 Betsunoh et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Betsunoh, Hironori
Fukuda, Takehiko
Anzai, Naohiko
Nishihara, Daisaku
Mizuno, Tomoya
Yuki, Hideo
Masuda, Akinori
Yamaguchi, Yoshiyuki
Abe, Hideyuki
Yashi, Masahiro
Fukabori, Yoshitatsu
Yoshida, Ken-Ichiro
Kamai, Takao
Increased expression of system large amino acid transporter (LAT)-1 mRNA is associated with invasive potential and unfavorable prognosis of human clear cell renal cell carcinoma
title Increased expression of system large amino acid transporter (LAT)-1 mRNA is associated with invasive potential and unfavorable prognosis of human clear cell renal cell carcinoma
title_full Increased expression of system large amino acid transporter (LAT)-1 mRNA is associated with invasive potential and unfavorable prognosis of human clear cell renal cell carcinoma
title_fullStr Increased expression of system large amino acid transporter (LAT)-1 mRNA is associated with invasive potential and unfavorable prognosis of human clear cell renal cell carcinoma
title_full_unstemmed Increased expression of system large amino acid transporter (LAT)-1 mRNA is associated with invasive potential and unfavorable prognosis of human clear cell renal cell carcinoma
title_short Increased expression of system large amino acid transporter (LAT)-1 mRNA is associated with invasive potential and unfavorable prognosis of human clear cell renal cell carcinoma
title_sort increased expression of system large amino acid transporter (lat)-1 mrna is associated with invasive potential and unfavorable prognosis of human clear cell renal cell carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3832879/
https://www.ncbi.nlm.nih.gov/pubmed/24168110
http://dx.doi.org/10.1186/1471-2407-13-509
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