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Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine
Purpose. Interstitial cells of Cajal (ICCs) are the pacemaker cells that generate slow waves in the gastrointestinal (GI) tract. We have aimed to investigate the effects of Socheongryong-Tang (SCRT) in ICCs from mouse's small intestine. Methods. The whole-cell patch-clamp configuration was used...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833007/ https://www.ncbi.nlm.nih.gov/pubmed/24288491 http://dx.doi.org/10.1155/2013/536350 |
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author | Hwang, Min Woo Lee, Hee Jung Song, Ho Joon Kim, Byung Joo |
author_facet | Hwang, Min Woo Lee, Hee Jung Song, Ho Joon Kim, Byung Joo |
author_sort | Hwang, Min Woo |
collection | PubMed |
description | Purpose. Interstitial cells of Cajal (ICCs) are the pacemaker cells that generate slow waves in the gastrointestinal (GI) tract. We have aimed to investigate the effects of Socheongryong-Tang (SCRT) in ICCs from mouse's small intestine. Methods. The whole-cell patch-clamp configuration was used to record membrane potentials from cultured ICCs. Intracellular Ca(2+) ([Ca(2+)](i)) increase was studied in cultured ICCs using fura-2 AM. Results. ICCs generated pacemaker potentials in mouse's small intestine. SCRT produced membrane depolarization in current clamp mode. Y25130 (5-HT(3) receptor antagonist) and RS39604 (5-HT(4) receptor antagonist) blocked SCRT-induced membrane depolarizations, whereas SB269970 (5-HT(7) receptor antagonist) did not. When GDP-β-S (1 mM) was in the pipette solution, SCRT did not induce the membrane depolarizations. [Ca(2+)](i) analysis showed that SCRT increased [Ca(2+)](i). In the presence of PD98059 (p42/44 MAPK inhibitor), SCRT did not produce membrane depolarizations. In addition, SB203580 (p38 MAPK inhibitor) and JNK inhibitors blocked the depolarizations by SCRT in pacemaker potentials. Furthermore, the membrane depolarizations by SCRT were not inhibited by U-73122, an active phospholipase C (PLC) inhibitor, but by U-73343, an inactive PLC inhibitor. Conclusion. These results suggest that SCRT might affect GI motility by the modulation of pacemaker activity through MAPKs and PLC pathways in the ICCs. |
format | Online Article Text |
id | pubmed-3833007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38330072013-11-28 Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine Hwang, Min Woo Lee, Hee Jung Song, Ho Joon Kim, Byung Joo ScientificWorldJournal Research Article Purpose. Interstitial cells of Cajal (ICCs) are the pacemaker cells that generate slow waves in the gastrointestinal (GI) tract. We have aimed to investigate the effects of Socheongryong-Tang (SCRT) in ICCs from mouse's small intestine. Methods. The whole-cell patch-clamp configuration was used to record membrane potentials from cultured ICCs. Intracellular Ca(2+) ([Ca(2+)](i)) increase was studied in cultured ICCs using fura-2 AM. Results. ICCs generated pacemaker potentials in mouse's small intestine. SCRT produced membrane depolarization in current clamp mode. Y25130 (5-HT(3) receptor antagonist) and RS39604 (5-HT(4) receptor antagonist) blocked SCRT-induced membrane depolarizations, whereas SB269970 (5-HT(7) receptor antagonist) did not. When GDP-β-S (1 mM) was in the pipette solution, SCRT did not induce the membrane depolarizations. [Ca(2+)](i) analysis showed that SCRT increased [Ca(2+)](i). In the presence of PD98059 (p42/44 MAPK inhibitor), SCRT did not produce membrane depolarizations. In addition, SB203580 (p38 MAPK inhibitor) and JNK inhibitors blocked the depolarizations by SCRT in pacemaker potentials. Furthermore, the membrane depolarizations by SCRT were not inhibited by U-73122, an active phospholipase C (PLC) inhibitor, but by U-73343, an inactive PLC inhibitor. Conclusion. These results suggest that SCRT might affect GI motility by the modulation of pacemaker activity through MAPKs and PLC pathways in the ICCs. Hindawi Publishing Corporation 2013-10-30 /pmc/articles/PMC3833007/ /pubmed/24288491 http://dx.doi.org/10.1155/2013/536350 Text en Copyright © 2013 Min Woo Hwang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hwang, Min Woo Lee, Hee Jung Song, Ho Joon Kim, Byung Joo Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine |
title | Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine |
title_full | Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine |
title_fullStr | Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine |
title_full_unstemmed | Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine |
title_short | Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine |
title_sort | involvement of mapks and plc pathways in modulation of pacemaking activity by so-cheong-ryong-tang in interstitial cells of cajal from murine small intestine |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833007/ https://www.ncbi.nlm.nih.gov/pubmed/24288491 http://dx.doi.org/10.1155/2013/536350 |
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