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PSF Knockdown Enhances Apoptosis via Downregulation of LC3B in Human Colon Cancer Cells

Our previous study demonstrated that PTB-associated splicing factor (PSF) is an important regulator of cell death and plays critical roles in the survival and growth of colon cancer cells. However, the molecular mechanism that activates these downstream signaling events remains unknown. To address t...

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Detalles Bibliográficos
Autores principales: Tsukahara, Tamotsu, Matsuda, Yoshikazu, Haniu, Hisao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833015/
https://www.ncbi.nlm.nih.gov/pubmed/24288667
http://dx.doi.org/10.1155/2013/204973
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author Tsukahara, Tamotsu
Matsuda, Yoshikazu
Haniu, Hisao
author_facet Tsukahara, Tamotsu
Matsuda, Yoshikazu
Haniu, Hisao
author_sort Tsukahara, Tamotsu
collection PubMed
description Our previous study demonstrated that PTB-associated splicing factor (PSF) is an important regulator of cell death and plays critical roles in the survival and growth of colon cancer cells. However, the molecular mechanism that activates these downstream signaling events remains unknown. To address this issue, we investigated the effects of PSF knockdown in two different colon cancer cell lines, DLD-1 and HT-29. We found that knockdown of PSF markedly decreased the autophagic molecule LC3B in DLD-1 cells but not in HT-29 cells. Furthermore, DLD-1 cells were more susceptible to PSF knockdown-induced cell growth inhibition and apoptosis than HT-29 cells. This susceptibility is probably a result of LC3B inhibition, given the known relationship between autophagy and apoptosis. C3B is associated with a number of physiological processes, including cell growth and apoptotic cell death. Our results suggest that autophagy is inhibited by PSF knockdown and that apoptosis and cell growth inhibition may act together to mediate the PSF-LC3B signaling pathway. Furthermore, we found that the peroxisome proliferator-activated receptor gamma (PPARγ)-PSF complex induced LC3B downregulation in DLD-1 cells. The results of this study identify a new physiological role for the PSF-LC3B axis as a potential endogenous modulator of colon cancer treatment.
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spelling pubmed-38330152013-11-28 PSF Knockdown Enhances Apoptosis via Downregulation of LC3B in Human Colon Cancer Cells Tsukahara, Tamotsu Matsuda, Yoshikazu Haniu, Hisao Biomed Res Int Research Article Our previous study demonstrated that PTB-associated splicing factor (PSF) is an important regulator of cell death and plays critical roles in the survival and growth of colon cancer cells. However, the molecular mechanism that activates these downstream signaling events remains unknown. To address this issue, we investigated the effects of PSF knockdown in two different colon cancer cell lines, DLD-1 and HT-29. We found that knockdown of PSF markedly decreased the autophagic molecule LC3B in DLD-1 cells but not in HT-29 cells. Furthermore, DLD-1 cells were more susceptible to PSF knockdown-induced cell growth inhibition and apoptosis than HT-29 cells. This susceptibility is probably a result of LC3B inhibition, given the known relationship between autophagy and apoptosis. C3B is associated with a number of physiological processes, including cell growth and apoptotic cell death. Our results suggest that autophagy is inhibited by PSF knockdown and that apoptosis and cell growth inhibition may act together to mediate the PSF-LC3B signaling pathway. Furthermore, we found that the peroxisome proliferator-activated receptor gamma (PPARγ)-PSF complex induced LC3B downregulation in DLD-1 cells. The results of this study identify a new physiological role for the PSF-LC3B axis as a potential endogenous modulator of colon cancer treatment. Hindawi Publishing Corporation 2013 2013-10-31 /pmc/articles/PMC3833015/ /pubmed/24288667 http://dx.doi.org/10.1155/2013/204973 Text en Copyright © 2013 Tamotsu Tsukahara et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tsukahara, Tamotsu
Matsuda, Yoshikazu
Haniu, Hisao
PSF Knockdown Enhances Apoptosis via Downregulation of LC3B in Human Colon Cancer Cells
title PSF Knockdown Enhances Apoptosis via Downregulation of LC3B in Human Colon Cancer Cells
title_full PSF Knockdown Enhances Apoptosis via Downregulation of LC3B in Human Colon Cancer Cells
title_fullStr PSF Knockdown Enhances Apoptosis via Downregulation of LC3B in Human Colon Cancer Cells
title_full_unstemmed PSF Knockdown Enhances Apoptosis via Downregulation of LC3B in Human Colon Cancer Cells
title_short PSF Knockdown Enhances Apoptosis via Downregulation of LC3B in Human Colon Cancer Cells
title_sort psf knockdown enhances apoptosis via downregulation of lc3b in human colon cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833015/
https://www.ncbi.nlm.nih.gov/pubmed/24288667
http://dx.doi.org/10.1155/2013/204973
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