Cargando…
Transplantation of Autologous Minced Bladder Mucosa for a One-Step Reconstruction of a Tissue Engineered Bladder Conduit
Surgical intervention is sometimes needed to create a conduit from the abdominal wall to the bladder for self-catheterization. We developed a method for tissue engineering a conduit for bladder emptying without in vitro cell culturing as a one-step procedure. In a porcine animal model bladder, wall...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833032/ https://www.ncbi.nlm.nih.gov/pubmed/24288669 http://dx.doi.org/10.1155/2013/212734 |
_version_ | 1782291776059473920 |
---|---|
author | Reinfeldt Engberg, Gisela Lundberg, Johan Chamorro, Clara Ibel Nordenskjöld, Agneta Fossum, Magdalena |
author_facet | Reinfeldt Engberg, Gisela Lundberg, Johan Chamorro, Clara Ibel Nordenskjöld, Agneta Fossum, Magdalena |
author_sort | Reinfeldt Engberg, Gisela |
collection | PubMed |
description | Surgical intervention is sometimes needed to create a conduit from the abdominal wall to the bladder for self-catheterization. We developed a method for tissue engineering a conduit for bladder emptying without in vitro cell culturing as a one-step procedure. In a porcine animal model bladder, wall tissue was excised and the mucosa was minced to small particles. The particles were attached to a tube in a 1 : 3 expansion rate with fibrin glue and transplanted back by attaching the tube to the bladder and through the abdominal wall. Sham served as controls. After 4-5 weeks, conduits were assessed in respect to macroscopic and microscopic appearance in 6 pigs. Two pigs underwent radiology before termination. Gross examination revealed a patent conduit with an opening to the bladder. Histology and immunostaining showed a multilayered transitional uroepithelium in all cases. Up to 89% of the luminal surface area was neoepithelialized but with a loose attachment to the submucosa. No epithelium was found in control animals. CT imaging revealed a patent channel that could be used for filling and emptying the bladder. Animals that experienced surgical complications did not form conduits. Minced autologous bladder mucosa can be transplanted around a tubular mold to create a conduit to the urinary bladder without in vitro culturing. |
format | Online Article Text |
id | pubmed-3833032 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38330322013-11-28 Transplantation of Autologous Minced Bladder Mucosa for a One-Step Reconstruction of a Tissue Engineered Bladder Conduit Reinfeldt Engberg, Gisela Lundberg, Johan Chamorro, Clara Ibel Nordenskjöld, Agneta Fossum, Magdalena Biomed Res Int Research Article Surgical intervention is sometimes needed to create a conduit from the abdominal wall to the bladder for self-catheterization. We developed a method for tissue engineering a conduit for bladder emptying without in vitro cell culturing as a one-step procedure. In a porcine animal model bladder, wall tissue was excised and the mucosa was minced to small particles. The particles were attached to a tube in a 1 : 3 expansion rate with fibrin glue and transplanted back by attaching the tube to the bladder and through the abdominal wall. Sham served as controls. After 4-5 weeks, conduits were assessed in respect to macroscopic and microscopic appearance in 6 pigs. Two pigs underwent radiology before termination. Gross examination revealed a patent conduit with an opening to the bladder. Histology and immunostaining showed a multilayered transitional uroepithelium in all cases. Up to 89% of the luminal surface area was neoepithelialized but with a loose attachment to the submucosa. No epithelium was found in control animals. CT imaging revealed a patent channel that could be used for filling and emptying the bladder. Animals that experienced surgical complications did not form conduits. Minced autologous bladder mucosa can be transplanted around a tubular mold to create a conduit to the urinary bladder without in vitro culturing. Hindawi Publishing Corporation 2013 2013-10-31 /pmc/articles/PMC3833032/ /pubmed/24288669 http://dx.doi.org/10.1155/2013/212734 Text en Copyright © 2013 Gisela Reinfeldt Engberg et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Reinfeldt Engberg, Gisela Lundberg, Johan Chamorro, Clara Ibel Nordenskjöld, Agneta Fossum, Magdalena Transplantation of Autologous Minced Bladder Mucosa for a One-Step Reconstruction of a Tissue Engineered Bladder Conduit |
title | Transplantation of Autologous Minced Bladder Mucosa for a One-Step Reconstruction of a Tissue Engineered Bladder Conduit |
title_full | Transplantation of Autologous Minced Bladder Mucosa for a One-Step Reconstruction of a Tissue Engineered Bladder Conduit |
title_fullStr | Transplantation of Autologous Minced Bladder Mucosa for a One-Step Reconstruction of a Tissue Engineered Bladder Conduit |
title_full_unstemmed | Transplantation of Autologous Minced Bladder Mucosa for a One-Step Reconstruction of a Tissue Engineered Bladder Conduit |
title_short | Transplantation of Autologous Minced Bladder Mucosa for a One-Step Reconstruction of a Tissue Engineered Bladder Conduit |
title_sort | transplantation of autologous minced bladder mucosa for a one-step reconstruction of a tissue engineered bladder conduit |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833032/ https://www.ncbi.nlm.nih.gov/pubmed/24288669 http://dx.doi.org/10.1155/2013/212734 |
work_keys_str_mv | AT reinfeldtengberggisela transplantationofautologousmincedbladdermucosaforaonestepreconstructionofatissueengineeredbladderconduit AT lundbergjohan transplantationofautologousmincedbladdermucosaforaonestepreconstructionofatissueengineeredbladderconduit AT chamorroclaraibel transplantationofautologousmincedbladdermucosaforaonestepreconstructionofatissueengineeredbladderconduit AT nordenskjoldagneta transplantationofautologousmincedbladdermucosaforaonestepreconstructionofatissueengineeredbladderconduit AT fossummagdalena transplantationofautologousmincedbladdermucosaforaonestepreconstructionofatissueengineeredbladderconduit |