Ultrahigh accuracy imaging modality for super-localization microscopy

Super-localization microscopy encompasses techniques that depend on the accurate localization of individual molecules from generally low light images. The obtainable localization accuracies, however, are ultimately limited by the image detector's pixelation and noise. We present the Ultrahigh Accuracy Imaging Modality (UAIM), which enables the attainment of accuracies approaching what is only achievable without detector pixelation and noise, and is experimentally observed to provide a >200% accuracy improvement over conventional imaging.