Ultrahigh accuracy imaging modality for super-localization microscopy

Super-localization microscopy encompasses techniques that depend on the accurate localization of individual molecules from generally low light images. The obtainable localization accuracies, however, are ultimately limited by the image detector's pixelation and noise. We present the Ultrahigh A...

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Detalles Bibliográficos
Autores principales: Chao, Jerry, Ram, Sripad, Ward, E. Sally, Ober, Raimund J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833351/
https://www.ncbi.nlm.nih.gov/pubmed/23455923
http://dx.doi.org/10.1038/nmeth.2396
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author Chao, Jerry
Ram, Sripad
Ward, E. Sally
Ober, Raimund J.
author_facet Chao, Jerry
Ram, Sripad
Ward, E. Sally
Ober, Raimund J.
author_sort Chao, Jerry
collection PubMed
description Super-localization microscopy encompasses techniques that depend on the accurate localization of individual molecules from generally low light images. The obtainable localization accuracies, however, are ultimately limited by the image detector's pixelation and noise. We present the Ultrahigh Accuracy Imaging Modality (UAIM), which enables the attainment of accuracies approaching what is only achievable without detector pixelation and noise, and is experimentally observed to provide a >200% accuracy improvement over conventional imaging.
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spelling pubmed-38333512013-11-19 Ultrahigh accuracy imaging modality for super-localization microscopy Chao, Jerry Ram, Sripad Ward, E. Sally Ober, Raimund J. Nat Methods Article Super-localization microscopy encompasses techniques that depend on the accurate localization of individual molecules from generally low light images. The obtainable localization accuracies, however, are ultimately limited by the image detector's pixelation and noise. We present the Ultrahigh Accuracy Imaging Modality (UAIM), which enables the attainment of accuracies approaching what is only achievable without detector pixelation and noise, and is experimentally observed to provide a >200% accuracy improvement over conventional imaging. 2013-03-03 2013-04 /pmc/articles/PMC3833351/ /pubmed/23455923 http://dx.doi.org/10.1038/nmeth.2396 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Chao, Jerry
Ram, Sripad
Ward, E. Sally
Ober, Raimund J.
Ultrahigh accuracy imaging modality for super-localization microscopy
title Ultrahigh accuracy imaging modality for super-localization microscopy
title_full Ultrahigh accuracy imaging modality for super-localization microscopy
title_fullStr Ultrahigh accuracy imaging modality for super-localization microscopy
title_full_unstemmed Ultrahigh accuracy imaging modality for super-localization microscopy
title_short Ultrahigh accuracy imaging modality for super-localization microscopy
title_sort ultrahigh accuracy imaging modality for super-localization microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833351/
https://www.ncbi.nlm.nih.gov/pubmed/23455923
http://dx.doi.org/10.1038/nmeth.2396
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AT wardesally ultrahighaccuracyimagingmodalityforsuperlocalizationmicroscopy
AT oberraimundj ultrahighaccuracyimagingmodalityforsuperlocalizationmicroscopy

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