Cargando…
Quercetin Induces Mitochondrial Biogenesis through Activation of HO-1 in HepG2 Cells
The regeneration of mitochondria by regulated biogenesis plays an important homeostatic role in cells and tissues and furthermore may provide an adaptive mechanism in certain diseases such as sepsis. The heme oxygenase (HO-1)/carbon monoxide (CO) system is an inducible cytoprotective mechanism in ma...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833383/ https://www.ncbi.nlm.nih.gov/pubmed/24288584 http://dx.doi.org/10.1155/2013/154279 |
_version_ | 1782291833013927936 |
---|---|
author | Rayamajhi, Nabin Kim, Seul-Ki Go, Hiroe Joe, Yeonsoo Callaway, Zak Kang, Jae-Gu Ryter, Stefan W. Chung, Hun Taeg |
author_facet | Rayamajhi, Nabin Kim, Seul-Ki Go, Hiroe Joe, Yeonsoo Callaway, Zak Kang, Jae-Gu Ryter, Stefan W. Chung, Hun Taeg |
author_sort | Rayamajhi, Nabin |
collection | PubMed |
description | The regeneration of mitochondria by regulated biogenesis plays an important homeostatic role in cells and tissues and furthermore may provide an adaptive mechanism in certain diseases such as sepsis. The heme oxygenase (HO-1)/carbon monoxide (CO) system is an inducible cytoprotective mechanism in mammalian cells. Natural antioxidants can provide therapeutic benefit, in part, by inducing the HO-1/CO system. This study focused on the mechanism by which the natural antioxidant quercetin can induce mitochondrial biogenesis in HepG2 cells. We found that quercetin treatment induced expression of mitochondrial biogenesis activators (PGC-1α, NRF-1, TFAM), mitochondrial DNA (mtDNA), and proteins (COX IV) in HepG2 cells. The HO inhibitor SnPP and the CO scavenger hemoglobin reversed the effects of quercetin on mitochondrial biogenesis in HepG2 cells. The stimulatory effects of quercetin on mitochondrial biogenesis could be recapitulated in vivo in liver tissue and antagonized by SnPP. Finally, quercetin conferred an anti-inflammatory effect in the liver of mice treated with LPS and prevented impairment of mitochondrial biogenesis by LPS in vivo. These salutary effects of quercetin in vivo were also antagonized by SnPP. Thus, our results suggest that quercetin enhances mitochondrial biogenesis mainly via the HO-1/CO system in vitro and in vivo. The beneficial effects of quercetin may provide a therapeutic basis in inflammatory diseases and sepsis. |
format | Online Article Text |
id | pubmed-3833383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38333832013-11-28 Quercetin Induces Mitochondrial Biogenesis through Activation of HO-1 in HepG2 Cells Rayamajhi, Nabin Kim, Seul-Ki Go, Hiroe Joe, Yeonsoo Callaway, Zak Kang, Jae-Gu Ryter, Stefan W. Chung, Hun Taeg Oxid Med Cell Longev Research Article The regeneration of mitochondria by regulated biogenesis plays an important homeostatic role in cells and tissues and furthermore may provide an adaptive mechanism in certain diseases such as sepsis. The heme oxygenase (HO-1)/carbon monoxide (CO) system is an inducible cytoprotective mechanism in mammalian cells. Natural antioxidants can provide therapeutic benefit, in part, by inducing the HO-1/CO system. This study focused on the mechanism by which the natural antioxidant quercetin can induce mitochondrial biogenesis in HepG2 cells. We found that quercetin treatment induced expression of mitochondrial biogenesis activators (PGC-1α, NRF-1, TFAM), mitochondrial DNA (mtDNA), and proteins (COX IV) in HepG2 cells. The HO inhibitor SnPP and the CO scavenger hemoglobin reversed the effects of quercetin on mitochondrial biogenesis in HepG2 cells. The stimulatory effects of quercetin on mitochondrial biogenesis could be recapitulated in vivo in liver tissue and antagonized by SnPP. Finally, quercetin conferred an anti-inflammatory effect in the liver of mice treated with LPS and prevented impairment of mitochondrial biogenesis by LPS in vivo. These salutary effects of quercetin in vivo were also antagonized by SnPP. Thus, our results suggest that quercetin enhances mitochondrial biogenesis mainly via the HO-1/CO system in vitro and in vivo. The beneficial effects of quercetin may provide a therapeutic basis in inflammatory diseases and sepsis. Hindawi Publishing Corporation 2013 2013-10-31 /pmc/articles/PMC3833383/ /pubmed/24288584 http://dx.doi.org/10.1155/2013/154279 Text en Copyright © 2013 Nabin Rayamajhi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Rayamajhi, Nabin Kim, Seul-Ki Go, Hiroe Joe, Yeonsoo Callaway, Zak Kang, Jae-Gu Ryter, Stefan W. Chung, Hun Taeg Quercetin Induces Mitochondrial Biogenesis through Activation of HO-1 in HepG2 Cells |
title | Quercetin Induces Mitochondrial Biogenesis through Activation of HO-1 in HepG2 Cells |
title_full | Quercetin Induces Mitochondrial Biogenesis through Activation of HO-1 in HepG2 Cells |
title_fullStr | Quercetin Induces Mitochondrial Biogenesis through Activation of HO-1 in HepG2 Cells |
title_full_unstemmed | Quercetin Induces Mitochondrial Biogenesis through Activation of HO-1 in HepG2 Cells |
title_short | Quercetin Induces Mitochondrial Biogenesis through Activation of HO-1 in HepG2 Cells |
title_sort | quercetin induces mitochondrial biogenesis through activation of ho-1 in hepg2 cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833383/ https://www.ncbi.nlm.nih.gov/pubmed/24288584 http://dx.doi.org/10.1155/2013/154279 |
work_keys_str_mv | AT rayamajhinabin quercetininducesmitochondrialbiogenesisthroughactivationofho1inhepg2cells AT kimseulki quercetininducesmitochondrialbiogenesisthroughactivationofho1inhepg2cells AT gohiroe quercetininducesmitochondrialbiogenesisthroughactivationofho1inhepg2cells AT joeyeonsoo quercetininducesmitochondrialbiogenesisthroughactivationofho1inhepg2cells AT callawayzak quercetininducesmitochondrialbiogenesisthroughactivationofho1inhepg2cells AT kangjaegu quercetininducesmitochondrialbiogenesisthroughactivationofho1inhepg2cells AT ryterstefanw quercetininducesmitochondrialbiogenesisthroughactivationofho1inhepg2cells AT chunghuntaeg quercetininducesmitochondrialbiogenesisthroughactivationofho1inhepg2cells |