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Efficient cellular fractionation improves RNA sequencing analysis of mature and nascent transcripts from human tissues
BACKGROUND: The starting material for RNA sequencing (RNA-seq) studies is usually total RNA or polyA+ RNA. Both forms of RNA represent heterogeneous pools of RNA molecules at different levels of maturation and processing. Such heterogeneity, in addition to the biases associated with polyA+ purificat...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833653/ https://www.ncbi.nlm.nih.gov/pubmed/24225116 http://dx.doi.org/10.1186/1472-6750-13-99 |