New method for visualization of C-heterochromatin in synaptonemal complex spreads

Abstract. DAPI staining of the metaphase chromosomes pretreated with barium hydroxide generates a C-like banding pattern. In this work a protocol for visualizing similar pattern at the synaptonemal complex (SC) spreads after immunostaining is suggested. This method was used to visualize centromeric...

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Detalles Bibliográficos
Autor principal: Lisachov, Artem P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833755/
https://www.ncbi.nlm.nih.gov/pubmed/24260695
http://dx.doi.org/10.3897/CompCytogen.v7i2.5187
Descripción
Sumario:Abstract. DAPI staining of the metaphase chromosomes pretreated with barium hydroxide generates a C-like banding pattern. In this work a protocol for visualizing similar pattern at the synaptonemal complex (SC) spreads after immunostaining is suggested. This method was used to visualize centromeric and sex heterochromatin at the SC spreads of guppy fish (Poecilia reticulata Peters, 1859). The efficiency of this method was further confirmed at SC spreads of the northern red-backed vole (Myodes rutilus (Pallas, 1779)), the guinea pig (Cavia porcellus (Linnaeus, 1758)), and the pigmy shrew (Sorex minutus Linnaeus, 1766).

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