New method for visualization of C-heterochromatin in synaptonemal complex spreads

Abstract. DAPI staining of the metaphase chromosomes pretreated with barium hydroxide generates a C-like banding pattern. In this work a protocol for visualizing similar pattern at the synaptonemal complex (SC) spreads after immunostaining is suggested. This method was used to visualize centromeric...

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Autor principal: Lisachov, Artem P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833755/
https://www.ncbi.nlm.nih.gov/pubmed/24260695
http://dx.doi.org/10.3897/CompCytogen.v7i2.5187
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author Lisachov, Artem P.
author_facet Lisachov, Artem P.
author_sort Lisachov, Artem P.
collection PubMed
description Abstract. DAPI staining of the metaphase chromosomes pretreated with barium hydroxide generates a C-like banding pattern. In this work a protocol for visualizing similar pattern at the synaptonemal complex (SC) spreads after immunostaining is suggested. This method was used to visualize centromeric and sex heterochromatin at the SC spreads of guppy fish (Poecilia reticulata Peters, 1859). The efficiency of this method was further confirmed at SC spreads of the northern red-backed vole (Myodes rutilus (Pallas, 1779)), the guinea pig (Cavia porcellus (Linnaeus, 1758)), and the pigmy shrew (Sorex minutus Linnaeus, 1766).
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spelling pubmed-38337552013-11-20 New method for visualization of C-heterochromatin in synaptonemal complex spreads Lisachov, Artem P. Comp Cytogenet Article Abstract. DAPI staining of the metaphase chromosomes pretreated with barium hydroxide generates a C-like banding pattern. In this work a protocol for visualizing similar pattern at the synaptonemal complex (SC) spreads after immunostaining is suggested. This method was used to visualize centromeric and sex heterochromatin at the SC spreads of guppy fish (Poecilia reticulata Peters, 1859). The efficiency of this method was further confirmed at SC spreads of the northern red-backed vole (Myodes rutilus (Pallas, 1779)), the guinea pig (Cavia porcellus (Linnaeus, 1758)), and the pigmy shrew (Sorex minutus Linnaeus, 1766). Pensoft Publishers 2013-05-22 /pmc/articles/PMC3833755/ /pubmed/24260695 http://dx.doi.org/10.3897/CompCytogen.v7i2.5187 Text en Artem P. Lisachov http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Lisachov, Artem P.
New method for visualization of C-heterochromatin in synaptonemal complex spreads
title New method for visualization of C-heterochromatin in synaptonemal complex spreads
title_full New method for visualization of C-heterochromatin in synaptonemal complex spreads
title_fullStr New method for visualization of C-heterochromatin in synaptonemal complex spreads
title_full_unstemmed New method for visualization of C-heterochromatin in synaptonemal complex spreads
title_short New method for visualization of C-heterochromatin in synaptonemal complex spreads
title_sort new method for visualization of c-heterochromatin in synaptonemal complex spreads
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833755/
https://www.ncbi.nlm.nih.gov/pubmed/24260695
http://dx.doi.org/10.3897/CompCytogen.v7i2.5187
work_keys_str_mv AT lisachovartemp newmethodforvisualizationofcheterochromatininsynaptonemalcomplexspreads

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