Cargando…
Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
Abstract. Thoracocharax stellatus (Characiformes, Gasteropelecidae) is a small Neotropical species of fish, widely distributed in several rivers of South America. Evidence for karyotype heteromorphysm in populations from different geographical regions has been reported for this species. In this way,...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pensoft Publishers
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833802/ https://www.ncbi.nlm.nih.gov/pubmed/24260672 http://dx.doi.org/10.3897/CompCytogen.v6i3.3637 |
_version_ | 1782291900448899072 |
---|---|
author | da Silva, Edson Lourenço de Borba, Rafael Splendore Centofante, Liano Miyazawa, Carlos Suetoshi Parise-Maltempi, Patrícia Pasquali |
author_facet | da Silva, Edson Lourenço de Borba, Rafael Splendore Centofante, Liano Miyazawa, Carlos Suetoshi Parise-Maltempi, Patrícia Pasquali |
author_sort | da Silva, Edson Lourenço |
collection | PubMed |
description | Abstract. Thoracocharax stellatus (Characiformes, Gasteropelecidae) is a small Neotropical species of fish, widely distributed in several rivers of South America. Evidence for karyotype heteromorphysm in populations from different geographical regions has been reported for this species. In this way, populations of Thoracocharax stellatus from the Paraguay River basin were cytogenetically characterized and the results were compared with other studies performed in the same species but from different basins. The results showed a diploid number of 2n = 54 for Thoracocharax stellatus, with chromosomes arranged in 6 metacentric (m), 6 submetacentric (sm), 2 subtelocentric (st) and 40 acrocentric (a), for both sexes, with a simple Nucleolus Organiser Region (NOR) system reported by the techniques of silver nitrate impregnation and fluorescent in situ hybridisation (FISH) using 18S rDNA sequences as probe. The distribution of constitutive heterochromatin, observed by the C-band technique and Chromomycin A3 staining showed great similarity among the analyzed populations and consists mainly of discrete blocks in the pericentromeric and telomeric regions of most chromosomes. The presence of female heterogamety was also observed indicating a ZZ/ZW system with W chromosome almost totally heterochromatic. The results also show cytogenetic diversity of the group and are useful to understand the mechanisms of karyotype evolution of the family. |
format | Online Article Text |
id | pubmed-3833802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Pensoft Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-38338022013-11-20 Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil da Silva, Edson Lourenço de Borba, Rafael Splendore Centofante, Liano Miyazawa, Carlos Suetoshi Parise-Maltempi, Patrícia Pasquali Comp Cytogenet Article Abstract. Thoracocharax stellatus (Characiformes, Gasteropelecidae) is a small Neotropical species of fish, widely distributed in several rivers of South America. Evidence for karyotype heteromorphysm in populations from different geographical regions has been reported for this species. In this way, populations of Thoracocharax stellatus from the Paraguay River basin were cytogenetically characterized and the results were compared with other studies performed in the same species but from different basins. The results showed a diploid number of 2n = 54 for Thoracocharax stellatus, with chromosomes arranged in 6 metacentric (m), 6 submetacentric (sm), 2 subtelocentric (st) and 40 acrocentric (a), for both sexes, with a simple Nucleolus Organiser Region (NOR) system reported by the techniques of silver nitrate impregnation and fluorescent in situ hybridisation (FISH) using 18S rDNA sequences as probe. The distribution of constitutive heterochromatin, observed by the C-band technique and Chromomycin A3 staining showed great similarity among the analyzed populations and consists mainly of discrete blocks in the pericentromeric and telomeric regions of most chromosomes. The presence of female heterogamety was also observed indicating a ZZ/ZW system with W chromosome almost totally heterochromatic. The results also show cytogenetic diversity of the group and are useful to understand the mechanisms of karyotype evolution of the family. Pensoft Publishers 2012-09-26 /pmc/articles/PMC3833802/ /pubmed/24260672 http://dx.doi.org/10.3897/CompCytogen.v6i3.3637 Text en Edson Lourenço da Silva, Rafael Splendore de Borba, Liano Centofante, Carlos Suetoshi Miyazawa, Patrícia Pasquali Parise-Maltempi http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Article da Silva, Edson Lourenço de Borba, Rafael Splendore Centofante, Liano Miyazawa, Carlos Suetoshi Parise-Maltempi, Patrícia Pasquali Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil |
title | Cytogenetic analysis in
Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil |
title_full | Cytogenetic analysis in
Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil |
title_fullStr | Cytogenetic analysis in
Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil |
title_full_unstemmed | Cytogenetic analysis in
Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil |
title_short | Cytogenetic analysis in
Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil |
title_sort | cytogenetic analysis in
thoracocharax stellatus (kner, 1858) (characiformes, gasteropelecidae) from paraguay river basin, mato grosso, brazil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833802/ https://www.ncbi.nlm.nih.gov/pubmed/24260672 http://dx.doi.org/10.3897/CompCytogen.v6i3.3637 |
work_keys_str_mv | AT dasilvaedsonlourenco cytogeneticanalysisinthoracocharaxstellatuskner1858characiformesgasteropelecidaefromparaguayriverbasinmatogrossobrazil AT deborbarafaelsplendore cytogeneticanalysisinthoracocharaxstellatuskner1858characiformesgasteropelecidaefromparaguayriverbasinmatogrossobrazil AT centofanteliano cytogeneticanalysisinthoracocharaxstellatuskner1858characiformesgasteropelecidaefromparaguayriverbasinmatogrossobrazil AT miyazawacarlossuetoshi cytogeneticanalysisinthoracocharaxstellatuskner1858characiformesgasteropelecidaefromparaguayriverbasinmatogrossobrazil AT parisemaltempipatriciapasquali cytogeneticanalysisinthoracocharaxstellatuskner1858characiformesgasteropelecidaefromparaguayriverbasinmatogrossobrazil |