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Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil

Abstract. Thoracocharax stellatus (Characiformes, Gasteropelecidae) is a small Neotropical species of fish, widely distributed in several rivers of South America. Evidence for karyotype heteromorphysm in populations from different geographical regions has been reported for this species. In this way,...

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Autores principales: da Silva, Edson Lourenço, de Borba, Rafael Splendore, Centofante, Liano, Miyazawa, Carlos Suetoshi, Parise-Maltempi, Patrícia Pasquali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833802/
https://www.ncbi.nlm.nih.gov/pubmed/24260672
http://dx.doi.org/10.3897/CompCytogen.v6i3.3637
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author da Silva, Edson Lourenço
de Borba, Rafael Splendore
Centofante, Liano
Miyazawa, Carlos Suetoshi
Parise-Maltempi, Patrícia Pasquali
author_facet da Silva, Edson Lourenço
de Borba, Rafael Splendore
Centofante, Liano
Miyazawa, Carlos Suetoshi
Parise-Maltempi, Patrícia Pasquali
author_sort da Silva, Edson Lourenço
collection PubMed
description Abstract. Thoracocharax stellatus (Characiformes, Gasteropelecidae) is a small Neotropical species of fish, widely distributed in several rivers of South America. Evidence for karyotype heteromorphysm in populations from different geographical regions has been reported for this species. In this way, populations of Thoracocharax stellatus from the Paraguay River basin were cytogenetically characterized and the results were compared with other studies performed in the same species but from different basins. The results showed a diploid number of 2n = 54 for Thoracocharax stellatus, with chromosomes arranged in 6 metacentric (m), 6 submetacentric (sm), 2 subtelocentric (st) and 40 acrocentric (a), for both sexes, with a simple Nucleolus Organiser Region (NOR) system reported by the techniques of silver nitrate impregnation and fluorescent in situ hybridisation (FISH) using 18S rDNA sequences as probe. The distribution of constitutive heterochromatin, observed by the C-band technique and Chromomycin A3 staining showed great similarity among the analyzed populations and consists mainly of discrete blocks in the pericentromeric and telomeric regions of most chromosomes. The presence of female heterogamety was also observed indicating a ZZ/ZW system with W chromosome almost totally heterochromatic. The results also show cytogenetic diversity of the group and are useful to understand the mechanisms of karyotype evolution of the family.
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spelling pubmed-38338022013-11-20 Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil da Silva, Edson Lourenço de Borba, Rafael Splendore Centofante, Liano Miyazawa, Carlos Suetoshi Parise-Maltempi, Patrícia Pasquali Comp Cytogenet Article Abstract. Thoracocharax stellatus (Characiformes, Gasteropelecidae) is a small Neotropical species of fish, widely distributed in several rivers of South America. Evidence for karyotype heteromorphysm in populations from different geographical regions has been reported for this species. In this way, populations of Thoracocharax stellatus from the Paraguay River basin were cytogenetically characterized and the results were compared with other studies performed in the same species but from different basins. The results showed a diploid number of 2n = 54 for Thoracocharax stellatus, with chromosomes arranged in 6 metacentric (m), 6 submetacentric (sm), 2 subtelocentric (st) and 40 acrocentric (a), for both sexes, with a simple Nucleolus Organiser Region (NOR) system reported by the techniques of silver nitrate impregnation and fluorescent in situ hybridisation (FISH) using 18S rDNA sequences as probe. The distribution of constitutive heterochromatin, observed by the C-band technique and Chromomycin A3 staining showed great similarity among the analyzed populations and consists mainly of discrete blocks in the pericentromeric and telomeric regions of most chromosomes. The presence of female heterogamety was also observed indicating a ZZ/ZW system with W chromosome almost totally heterochromatic. The results also show cytogenetic diversity of the group and are useful to understand the mechanisms of karyotype evolution of the family. Pensoft Publishers 2012-09-26 /pmc/articles/PMC3833802/ /pubmed/24260672 http://dx.doi.org/10.3897/CompCytogen.v6i3.3637 Text en Edson Lourenço da Silva, Rafael Splendore de Borba, Liano Centofante, Carlos Suetoshi Miyazawa, Patrícia Pasquali Parise-Maltempi http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
da Silva, Edson Lourenço
de Borba, Rafael Splendore
Centofante, Liano
Miyazawa, Carlos Suetoshi
Parise-Maltempi, Patrícia Pasquali
Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
title Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
title_full Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
title_fullStr Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
title_full_unstemmed Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
title_short Cytogenetic analysis in Thoracocharax stellatus (Kner, 1858) (Characiformes, Gasteropelecidae) from Paraguay River Basin, Mato Grosso, Brazil
title_sort cytogenetic analysis in thoracocharax stellatus (kner, 1858) (characiformes, gasteropelecidae) from paraguay river basin, mato grosso, brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833802/
https://www.ncbi.nlm.nih.gov/pubmed/24260672
http://dx.doi.org/10.3897/CompCytogen.v6i3.3637
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