Outbreak of Bioaerosols with Continuous Use of Humidifier in Apartment Room

The effect of continuous humidifier use on the bioaerosol concentration in an indoor environment was investigated. An ultrasonic humidifier was operated for 10 hr per day for 15 days in an apartment room. During this time period, viable bioaerosol samples were taken using a single-stage Andersen sampler containing culture media plates for bacteria and fungi. The culture plates were then incubated at room temperature for 2~7 days depending on the media. The counts for the air sample plates were corrected for multiple impactions using the positive hole conversion method and are reported as the colony forming units per cubic meter of air (CFU/m(3)). While the bacterial concentration measured using the tryptic soy agar (TSA) did not show any significant change during the first 3 days, the concentration increased from the 6(th) day (6979 CFU/m(3)) and reached a maximum on the 9(th) day (46431 CFU/m(3)). The concentration then decreased to 2470 CFU/m(3) on the 12(th) day, at which point the fungal concentration increased rapidly to 14424~16038 CFU/m(3). Also, while the fungal concentration showed a significant change until the 9(th) day of humidifier use, fungal growth was observed on the wallpaper and increased rapidly from the 12(th) day. However, the bacterial concentration increased rapidly after the fungi were removed by remediation. The major fungal species identified in the samples were Penicillium representing 34%, Aspergillus representing 31%, Cladosporium representing 24%, and Alternaria representing 1%. The results also indicated that a relative humidity over 80% was easily achieved with continuous humidifier use. Yet, maintaining a high humidity in a room can cause a rapid outbreak of microbial growth.