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Genome-Wide Transcriptional Response During the Development of Bleomycin-Induced Pulmonary Fibrosis in Sprague-Dawley Rats

Pulmonary fibrosis is a common consequence of many lung diseases and a leading cause of morbidity and mortality. The molecular mechanisms underlying the development of pulmonary fibrosis remain poorly understood. One model used successfully to study pulmonary fibrosis over the past few decades is th...

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Autores principales: Park, Han-Jin, Yang, Mi-Jin, Oh, Jung-Hwa, Yang, Young-Su, Kwon, Myung-Sang, Song, Chang Woo, Yoon, Seokjoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Toxicology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834473/
https://www.ncbi.nlm.nih.gov/pubmed/24278517
http://dx.doi.org/10.5487/TR.2010.26.2.137
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author Park, Han-Jin
Yang, Mi-Jin
Oh, Jung-Hwa
Yang, Young-Su
Kwon, Myung-Sang
Song, Chang Woo
Yoon, Seokjoo
author_facet Park, Han-Jin
Yang, Mi-Jin
Oh, Jung-Hwa
Yang, Young-Su
Kwon, Myung-Sang
Song, Chang Woo
Yoon, Seokjoo
author_sort Park, Han-Jin
collection PubMed
description Pulmonary fibrosis is a common consequence of many lung diseases and a leading cause of morbidity and mortality. The molecular mechanisms underlying the development of pulmonary fibrosis remain poorly understood. One model used successfully to study pulmonary fibrosis over the past few decades is the bleomycin-induced pulmonary fibrosis model. We aimed to identify the genes associated with fibrogenesis using an Affymetrix GeneChip system in a bleomycin-induced rat model for pulmonary fibrosis. To confirm fibrosis development, several analyses were performed, including cellular evaluations using bronchoalveolar lavage fluid, measurement of lactate dehydrogenase activity, and histopathological examinations. Common aspects of pulmonary fibrosis such as prolonged inflammation, immune cell infiltration, emergence of fibroblasts, and deposition of extracellular matrix and connective tissue elements were observed. Global gene expression analysis revealed significantly altered expression of genes (≥ 1.5-fold, p < 0.05.) in a time-dependent manner during the development of pulmonary fibrosis. Our results are consistent with previous results of well-documented gene expression. Interestingly, the expression of triggering receptor expressed on myeloid cells 2 (Trem2) , secreted phosphoprotein 1 (Spp1) , and several proteases such as Tpsab1, Mcpt1, and Cma1 was considerably induced in the lung after bleomycin treatment, despite little evidence that they are involved in pulmonary fibrogenesis. These data will aid in our understanding of fibrogenic mechanisms and contribute to the identification of candidate biomarkers of fibrotic disease development.
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spelling pubmed-38344732013-11-25 Genome-Wide Transcriptional Response During the Development of Bleomycin-Induced Pulmonary Fibrosis in Sprague-Dawley Rats Park, Han-Jin Yang, Mi-Jin Oh, Jung-Hwa Yang, Young-Su Kwon, Myung-Sang Song, Chang Woo Yoon, Seokjoo Toxicol Res Article Pulmonary fibrosis is a common consequence of many lung diseases and a leading cause of morbidity and mortality. The molecular mechanisms underlying the development of pulmonary fibrosis remain poorly understood. One model used successfully to study pulmonary fibrosis over the past few decades is the bleomycin-induced pulmonary fibrosis model. We aimed to identify the genes associated with fibrogenesis using an Affymetrix GeneChip system in a bleomycin-induced rat model for pulmonary fibrosis. To confirm fibrosis development, several analyses were performed, including cellular evaluations using bronchoalveolar lavage fluid, measurement of lactate dehydrogenase activity, and histopathological examinations. Common aspects of pulmonary fibrosis such as prolonged inflammation, immune cell infiltration, emergence of fibroblasts, and deposition of extracellular matrix and connective tissue elements were observed. Global gene expression analysis revealed significantly altered expression of genes (≥ 1.5-fold, p < 0.05.) in a time-dependent manner during the development of pulmonary fibrosis. Our results are consistent with previous results of well-documented gene expression. Interestingly, the expression of triggering receptor expressed on myeloid cells 2 (Trem2) , secreted phosphoprotein 1 (Spp1) , and several proteases such as Tpsab1, Mcpt1, and Cma1 was considerably induced in the lung after bleomycin treatment, despite little evidence that they are involved in pulmonary fibrogenesis. These data will aid in our understanding of fibrogenic mechanisms and contribute to the identification of candidate biomarkers of fibrotic disease development. The Korean Society of Toxicology 2010-06 /pmc/articles/PMC3834473/ /pubmed/24278517 http://dx.doi.org/10.5487/TR.2010.26.2.137 Text en Copyright ©2010, The Korean Society of Toxicology
spellingShingle Article
Park, Han-Jin
Yang, Mi-Jin
Oh, Jung-Hwa
Yang, Young-Su
Kwon, Myung-Sang
Song, Chang Woo
Yoon, Seokjoo
Genome-Wide Transcriptional Response During the Development of Bleomycin-Induced Pulmonary Fibrosis in Sprague-Dawley Rats
title Genome-Wide Transcriptional Response During the Development of Bleomycin-Induced Pulmonary Fibrosis in Sprague-Dawley Rats
title_full Genome-Wide Transcriptional Response During the Development of Bleomycin-Induced Pulmonary Fibrosis in Sprague-Dawley Rats
title_fullStr Genome-Wide Transcriptional Response During the Development of Bleomycin-Induced Pulmonary Fibrosis in Sprague-Dawley Rats
title_full_unstemmed Genome-Wide Transcriptional Response During the Development of Bleomycin-Induced Pulmonary Fibrosis in Sprague-Dawley Rats
title_short Genome-Wide Transcriptional Response During the Development of Bleomycin-Induced Pulmonary Fibrosis in Sprague-Dawley Rats
title_sort genome-wide transcriptional response during the development of bleomycin-induced pulmonary fibrosis in sprague-dawley rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834473/
https://www.ncbi.nlm.nih.gov/pubmed/24278517
http://dx.doi.org/10.5487/TR.2010.26.2.137
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