Cargando…
Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line
The cytochrome P450 (P450, CYP) are the superfamily of heme-containing monooxygenase enzymes, found throughout all nature including mammals, plants, and microorganisms. Mammalian P450 enzymes are involved in oxidative metabolism of a wide range of endo- and exogenous chemicals. Especially P450s invo...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Toxicology
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834515/ https://www.ncbi.nlm.nih.gov/pubmed/24278547 http://dx.doi.org/10.5487/TR.2011.27.1.025 |
_version_ | 1782292006297403392 |
---|---|
author | Lee, Hye Ja Park, Mi Kyung Park, Young Ran Kim, Donghak Yun, Chul-Ho Chun, Young-Jin Shin, Hee Jung Na, Han Sung Chung, Myeon Woo Lee, Chang Hoon |
author_facet | Lee, Hye Ja Park, Mi Kyung Park, Young Ran Kim, Donghak Yun, Chul-Ho Chun, Young-Jin Shin, Hee Jung Na, Han Sung Chung, Myeon Woo Lee, Chang Hoon |
author_sort | Lee, Hye Ja |
collection | PubMed |
description | The cytochrome P450 (P450, CYP) are the superfamily of heme-containing monooxygenase enzymes, found throughout all nature including mammals, plants, and microorganisms. Mammalian P450 enzymes are involved in oxidative metabolism of a wide range of endo- and exogenous chemicals. Especially P450s involved in drug metabolisms are important for drug efficacy and polymorphisms of P450s in individuals reflect differences of drug responses between people. To study the functional differences of CYP2A6, CYP2D6, and CYP4A11 variants, we cloned the four CYP2A6, three CYP2D6, and three CYP4A11 variants, which were found in Korean populations, in mammalian expression vector pcDNA by PCR and examined their expressions in COS-7 mammalian cells using immunoblots using P450 specific polyclonal antibodies. Three of four CYP2A6, two of three CYP4A11, and two of three CYP2D6 variants showed expressions in COS-7 cells but the relative levels of expressions are remarkably different in those of each variants. Our findings may help to study and explain the differences between functions of CYP variants and drug responses in Korean populations. |
format | Online Article Text |
id | pubmed-3834515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Korean Society of Toxicology |
record_format | MEDLINE/PubMed |
spelling | pubmed-38345152013-11-25 Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line Lee, Hye Ja Park, Mi Kyung Park, Young Ran Kim, Donghak Yun, Chul-Ho Chun, Young-Jin Shin, Hee Jung Na, Han Sung Chung, Myeon Woo Lee, Chang Hoon Toxicol Res Article The cytochrome P450 (P450, CYP) are the superfamily of heme-containing monooxygenase enzymes, found throughout all nature including mammals, plants, and microorganisms. Mammalian P450 enzymes are involved in oxidative metabolism of a wide range of endo- and exogenous chemicals. Especially P450s involved in drug metabolisms are important for drug efficacy and polymorphisms of P450s in individuals reflect differences of drug responses between people. To study the functional differences of CYP2A6, CYP2D6, and CYP4A11 variants, we cloned the four CYP2A6, three CYP2D6, and three CYP4A11 variants, which were found in Korean populations, in mammalian expression vector pcDNA by PCR and examined their expressions in COS-7 mammalian cells using immunoblots using P450 specific polyclonal antibodies. Three of four CYP2A6, two of three CYP4A11, and two of three CYP2D6 variants showed expressions in COS-7 cells but the relative levels of expressions are remarkably different in those of each variants. Our findings may help to study and explain the differences between functions of CYP variants and drug responses in Korean populations. The Korean Society of Toxicology 2011-03 /pmc/articles/PMC3834515/ /pubmed/24278547 http://dx.doi.org/10.5487/TR.2011.27.1.025 Text en Copyright ©2011, The Korean Society of Toxicology |
spellingShingle | Article Lee, Hye Ja Park, Mi Kyung Park, Young Ran Kim, Donghak Yun, Chul-Ho Chun, Young-Jin Shin, Hee Jung Na, Han Sung Chung, Myeon Woo Lee, Chang Hoon Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line |
title | Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line |
title_full | Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line |
title_fullStr | Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line |
title_full_unstemmed | Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line |
title_short | Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line |
title_sort | expression of cyp2a6, cyp2d6 and cyp4a11 polymorphisms in cos7 mammalian cell line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834515/ https://www.ncbi.nlm.nih.gov/pubmed/24278547 http://dx.doi.org/10.5487/TR.2011.27.1.025 |
work_keys_str_mv | AT leehyeja expressionofcyp2a6cyp2d6andcyp4a11polymorphismsincos7mammaliancellline AT parkmikyung expressionofcyp2a6cyp2d6andcyp4a11polymorphismsincos7mammaliancellline AT parkyoungran expressionofcyp2a6cyp2d6andcyp4a11polymorphismsincos7mammaliancellline AT kimdonghak expressionofcyp2a6cyp2d6andcyp4a11polymorphismsincos7mammaliancellline AT yunchulho expressionofcyp2a6cyp2d6andcyp4a11polymorphismsincos7mammaliancellline AT chunyoungjin expressionofcyp2a6cyp2d6andcyp4a11polymorphismsincos7mammaliancellline AT shinheejung expressionofcyp2a6cyp2d6andcyp4a11polymorphismsincos7mammaliancellline AT nahansung expressionofcyp2a6cyp2d6andcyp4a11polymorphismsincos7mammaliancellline AT chungmyeonwoo expressionofcyp2a6cyp2d6andcyp4a11polymorphismsincos7mammaliancellline AT leechanghoon expressionofcyp2a6cyp2d6andcyp4a11polymorphismsincos7mammaliancellline |