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Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line

The cytochrome P450 (P450, CYP) are the superfamily of heme-containing monooxygenase enzymes, found throughout all nature including mammals, plants, and microorganisms. Mammalian P450 enzymes are involved in oxidative metabolism of a wide range of endo- and exogenous chemicals. Especially P450s invo...

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Autores principales: Lee, Hye Ja, Park, Mi Kyung, Park, Young Ran, Kim, Donghak, Yun, Chul-Ho, Chun, Young-Jin, Shin, Hee Jung, Na, Han Sung, Chung, Myeon Woo, Lee, Chang Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Toxicology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834515/
https://www.ncbi.nlm.nih.gov/pubmed/24278547
http://dx.doi.org/10.5487/TR.2011.27.1.025
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author Lee, Hye Ja
Park, Mi Kyung
Park, Young Ran
Kim, Donghak
Yun, Chul-Ho
Chun, Young-Jin
Shin, Hee Jung
Na, Han Sung
Chung, Myeon Woo
Lee, Chang Hoon
author_facet Lee, Hye Ja
Park, Mi Kyung
Park, Young Ran
Kim, Donghak
Yun, Chul-Ho
Chun, Young-Jin
Shin, Hee Jung
Na, Han Sung
Chung, Myeon Woo
Lee, Chang Hoon
author_sort Lee, Hye Ja
collection PubMed
description The cytochrome P450 (P450, CYP) are the superfamily of heme-containing monooxygenase enzymes, found throughout all nature including mammals, plants, and microorganisms. Mammalian P450 enzymes are involved in oxidative metabolism of a wide range of endo- and exogenous chemicals. Especially P450s involved in drug metabolisms are important for drug efficacy and polymorphisms of P450s in individuals reflect differences of drug responses between people. To study the functional differences of CYP2A6, CYP2D6, and CYP4A11 variants, we cloned the four CYP2A6, three CYP2D6, and three CYP4A11 variants, which were found in Korean populations, in mammalian expression vector pcDNA by PCR and examined their expressions in COS-7 mammalian cells using immunoblots using P450 specific polyclonal antibodies. Three of four CYP2A6, two of three CYP4A11, and two of three CYP2D6 variants showed expressions in COS-7 cells but the relative levels of expressions are remarkably different in those of each variants. Our findings may help to study and explain the differences between functions of CYP variants and drug responses in Korean populations.
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spelling pubmed-38345152013-11-25 Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line Lee, Hye Ja Park, Mi Kyung Park, Young Ran Kim, Donghak Yun, Chul-Ho Chun, Young-Jin Shin, Hee Jung Na, Han Sung Chung, Myeon Woo Lee, Chang Hoon Toxicol Res Article The cytochrome P450 (P450, CYP) are the superfamily of heme-containing monooxygenase enzymes, found throughout all nature including mammals, plants, and microorganisms. Mammalian P450 enzymes are involved in oxidative metabolism of a wide range of endo- and exogenous chemicals. Especially P450s involved in drug metabolisms are important for drug efficacy and polymorphisms of P450s in individuals reflect differences of drug responses between people. To study the functional differences of CYP2A6, CYP2D6, and CYP4A11 variants, we cloned the four CYP2A6, three CYP2D6, and three CYP4A11 variants, which were found in Korean populations, in mammalian expression vector pcDNA by PCR and examined their expressions in COS-7 mammalian cells using immunoblots using P450 specific polyclonal antibodies. Three of four CYP2A6, two of three CYP4A11, and two of three CYP2D6 variants showed expressions in COS-7 cells but the relative levels of expressions are remarkably different in those of each variants. Our findings may help to study and explain the differences between functions of CYP variants and drug responses in Korean populations. The Korean Society of Toxicology 2011-03 /pmc/articles/PMC3834515/ /pubmed/24278547 http://dx.doi.org/10.5487/TR.2011.27.1.025 Text en Copyright ©2011, The Korean Society of Toxicology
spellingShingle Article
Lee, Hye Ja
Park, Mi Kyung
Park, Young Ran
Kim, Donghak
Yun, Chul-Ho
Chun, Young-Jin
Shin, Hee Jung
Na, Han Sung
Chung, Myeon Woo
Lee, Chang Hoon
Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line
title Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line
title_full Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line
title_fullStr Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line
title_full_unstemmed Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line
title_short Expression of CYP2A6, CYP2D6 and CYP4A11 Polymorphisms in COS7 Mammalian Cell Line
title_sort expression of cyp2a6, cyp2d6 and cyp4a11 polymorphisms in cos7 mammalian cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834515/
https://www.ncbi.nlm.nih.gov/pubmed/24278547
http://dx.doi.org/10.5487/TR.2011.27.1.025
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