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Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma
BACKGROUND: The lung squamous cell carcinoma survival rate is very poor despite multimodal treatment. It is urgent to discover novel candidate biomarkers for prognostic assessment and therapeutic targets to lung squamous cell carcinoma (SCC). RESULTS: Herein a two-dimensional gel electrophoresis and...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834881/ https://www.ncbi.nlm.nih.gov/pubmed/24139065 http://dx.doi.org/10.1186/1476-4598-12-125 |
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author | Gong, Fengming Peng, Xingchen Luo, Can Shen, Guobo Zhao, Chengjian Zou, Liqun Li, Longhao Sang, Yaxiong Zhao, Yuwei Zhao, Xia |
author_facet | Gong, Fengming Peng, Xingchen Luo, Can Shen, Guobo Zhao, Chengjian Zou, Liqun Li, Longhao Sang, Yaxiong Zhao, Yuwei Zhao, Xia |
author_sort | Gong, Fengming |
collection | PubMed |
description | BACKGROUND: The lung squamous cell carcinoma survival rate is very poor despite multimodal treatment. It is urgent to discover novel candidate biomarkers for prognostic assessment and therapeutic targets to lung squamous cell carcinoma (SCC). RESULTS: Herein a two-dimensional gel electrophoresis and ESI-Q-TOF MS/MS-based proteomic approach was used to identify differentially expressed proteins between lung SCC and adjacent normal tissues. 31 proteins with significant alteration were identified. These proteins were mainly involved in metabolism, calcium ion binding, signal transduction and so on. Cathepsin B (CTSB) was one of the most significantly altered proteins and was confirmed by western blotting. Immunohistochemistry showed the correlation between higher CTSB expression and lower survival rate. No statistically significant difference between CTSB-shRNA treated group and the controls was observed in tumor volume, tumor weight, proliferation and apoptosis. However, the CTSB-shRNA significantly inhibited tumor metastases and prolonged survival in LL/2 metastatic model. Moreover, CTSB, Shh and Ptch were up-regulated in patients with metastatic lung SCC, suggesting that hedgehog signaling might be activated in metastatic lung SCC which could affect the expression of CTSB that influence the invasive activity of lung SCC. CONCLUSIONS: These data suggested that CTSB might serve as a prognostic and therapeutic marker for lung SCC. |
format | Online Article Text |
id | pubmed-3834881 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38348812013-11-21 Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma Gong, Fengming Peng, Xingchen Luo, Can Shen, Guobo Zhao, Chengjian Zou, Liqun Li, Longhao Sang, Yaxiong Zhao, Yuwei Zhao, Xia Mol Cancer Research BACKGROUND: The lung squamous cell carcinoma survival rate is very poor despite multimodal treatment. It is urgent to discover novel candidate biomarkers for prognostic assessment and therapeutic targets to lung squamous cell carcinoma (SCC). RESULTS: Herein a two-dimensional gel electrophoresis and ESI-Q-TOF MS/MS-based proteomic approach was used to identify differentially expressed proteins between lung SCC and adjacent normal tissues. 31 proteins with significant alteration were identified. These proteins were mainly involved in metabolism, calcium ion binding, signal transduction and so on. Cathepsin B (CTSB) was one of the most significantly altered proteins and was confirmed by western blotting. Immunohistochemistry showed the correlation between higher CTSB expression and lower survival rate. No statistically significant difference between CTSB-shRNA treated group and the controls was observed in tumor volume, tumor weight, proliferation and apoptosis. However, the CTSB-shRNA significantly inhibited tumor metastases and prolonged survival in LL/2 metastatic model. Moreover, CTSB, Shh and Ptch were up-regulated in patients with metastatic lung SCC, suggesting that hedgehog signaling might be activated in metastatic lung SCC which could affect the expression of CTSB that influence the invasive activity of lung SCC. CONCLUSIONS: These data suggested that CTSB might serve as a prognostic and therapeutic marker for lung SCC. BioMed Central 2013-10-20 /pmc/articles/PMC3834881/ /pubmed/24139065 http://dx.doi.org/10.1186/1476-4598-12-125 Text en Copyright © 2013 Gong et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Gong, Fengming Peng, Xingchen Luo, Can Shen, Guobo Zhao, Chengjian Zou, Liqun Li, Longhao Sang, Yaxiong Zhao, Yuwei Zhao, Xia Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma |
title | Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma |
title_full | Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma |
title_fullStr | Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma |
title_full_unstemmed | Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma |
title_short | Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma |
title_sort | cathepsin b as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834881/ https://www.ncbi.nlm.nih.gov/pubmed/24139065 http://dx.doi.org/10.1186/1476-4598-12-125 |
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