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Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma

BACKGROUND: The lung squamous cell carcinoma survival rate is very poor despite multimodal treatment. It is urgent to discover novel candidate biomarkers for prognostic assessment and therapeutic targets to lung squamous cell carcinoma (SCC). RESULTS: Herein a two-dimensional gel electrophoresis and...

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Autores principales: Gong, Fengming, Peng, Xingchen, Luo, Can, Shen, Guobo, Zhao, Chengjian, Zou, Liqun, Li, Longhao, Sang, Yaxiong, Zhao, Yuwei, Zhao, Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834881/
https://www.ncbi.nlm.nih.gov/pubmed/24139065
http://dx.doi.org/10.1186/1476-4598-12-125
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author Gong, Fengming
Peng, Xingchen
Luo, Can
Shen, Guobo
Zhao, Chengjian
Zou, Liqun
Li, Longhao
Sang, Yaxiong
Zhao, Yuwei
Zhao, Xia
author_facet Gong, Fengming
Peng, Xingchen
Luo, Can
Shen, Guobo
Zhao, Chengjian
Zou, Liqun
Li, Longhao
Sang, Yaxiong
Zhao, Yuwei
Zhao, Xia
author_sort Gong, Fengming
collection PubMed
description BACKGROUND: The lung squamous cell carcinoma survival rate is very poor despite multimodal treatment. It is urgent to discover novel candidate biomarkers for prognostic assessment and therapeutic targets to lung squamous cell carcinoma (SCC). RESULTS: Herein a two-dimensional gel electrophoresis and ESI-Q-TOF MS/MS-based proteomic approach was used to identify differentially expressed proteins between lung SCC and adjacent normal tissues. 31 proteins with significant alteration were identified. These proteins were mainly involved in metabolism, calcium ion binding, signal transduction and so on. Cathepsin B (CTSB) was one of the most significantly altered proteins and was confirmed by western blotting. Immunohistochemistry showed the correlation between higher CTSB expression and lower survival rate. No statistically significant difference between CTSB-shRNA treated group and the controls was observed in tumor volume, tumor weight, proliferation and apoptosis. However, the CTSB-shRNA significantly inhibited tumor metastases and prolonged survival in LL/2 metastatic model. Moreover, CTSB, Shh and Ptch were up-regulated in patients with metastatic lung SCC, suggesting that hedgehog signaling might be activated in metastatic lung SCC which could affect the expression of CTSB that influence the invasive activity of lung SCC. CONCLUSIONS: These data suggested that CTSB might serve as a prognostic and therapeutic marker for lung SCC.
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spelling pubmed-38348812013-11-21 Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma Gong, Fengming Peng, Xingchen Luo, Can Shen, Guobo Zhao, Chengjian Zou, Liqun Li, Longhao Sang, Yaxiong Zhao, Yuwei Zhao, Xia Mol Cancer Research BACKGROUND: The lung squamous cell carcinoma survival rate is very poor despite multimodal treatment. It is urgent to discover novel candidate biomarkers for prognostic assessment and therapeutic targets to lung squamous cell carcinoma (SCC). RESULTS: Herein a two-dimensional gel electrophoresis and ESI-Q-TOF MS/MS-based proteomic approach was used to identify differentially expressed proteins between lung SCC and adjacent normal tissues. 31 proteins with significant alteration were identified. These proteins were mainly involved in metabolism, calcium ion binding, signal transduction and so on. Cathepsin B (CTSB) was one of the most significantly altered proteins and was confirmed by western blotting. Immunohistochemistry showed the correlation between higher CTSB expression and lower survival rate. No statistically significant difference between CTSB-shRNA treated group and the controls was observed in tumor volume, tumor weight, proliferation and apoptosis. However, the CTSB-shRNA significantly inhibited tumor metastases and prolonged survival in LL/2 metastatic model. Moreover, CTSB, Shh and Ptch were up-regulated in patients with metastatic lung SCC, suggesting that hedgehog signaling might be activated in metastatic lung SCC which could affect the expression of CTSB that influence the invasive activity of lung SCC. CONCLUSIONS: These data suggested that CTSB might serve as a prognostic and therapeutic marker for lung SCC. BioMed Central 2013-10-20 /pmc/articles/PMC3834881/ /pubmed/24139065 http://dx.doi.org/10.1186/1476-4598-12-125 Text en Copyright © 2013 Gong et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Gong, Fengming
Peng, Xingchen
Luo, Can
Shen, Guobo
Zhao, Chengjian
Zou, Liqun
Li, Longhao
Sang, Yaxiong
Zhao, Yuwei
Zhao, Xia
Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma
title Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma
title_full Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma
title_fullStr Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma
title_full_unstemmed Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma
title_short Cathepsin B as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma
title_sort cathepsin b as a potential prognostic and therapeutic marker for human lung squamous cell carcinoma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834881/
https://www.ncbi.nlm.nih.gov/pubmed/24139065
http://dx.doi.org/10.1186/1476-4598-12-125
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