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Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery
The aim of the present study was to evaluate the transfection potential of chitosan-coated, green-fluorescent magnetic nanoparticles (MNPs) (chi-MNPs) after encapsulation inside polyethylglycol (PEG)ylated liposomes that produced lipid-encapsulated chitosan-coated MNPs (lip-MNPs), and also to evalua...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834948/ https://www.ncbi.nlm.nih.gov/pubmed/24300449 http://dx.doi.org/10.3390/pharmaceutics5020246 |
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author | Linemann, Thomas Thomsen, Louiza B. Du Jardin, Kristian G. Laursen, Jens C. Jensen, Jesper B. Lichota, Jacek Moos, Torben |
author_facet | Linemann, Thomas Thomsen, Louiza B. Du Jardin, Kristian G. Laursen, Jens C. Jensen, Jesper B. Lichota, Jacek Moos, Torben |
author_sort | Linemann, Thomas |
collection | PubMed |
description | The aim of the present study was to evaluate the transfection potential of chitosan-coated, green-fluorescent magnetic nanoparticles (MNPs) (chi-MNPs) after encapsulation inside polyethylglycol (PEG)ylated liposomes that produced lipid-encapsulated chitosan-coated MNPs (lip-MNPs), and also to evaluate how these particles would distribute in vivo after systemic injection. The transfection potential of both chi-MNPs and lip-MNPs was evaluated in vitro in rat brain endothelial 4 (RBE4) cells with and without applying a magnetic field. Subsequently, the MNPs were evaluated in vivo in young rats. The in vitro investigations revealed that the application of a magnetic field resulted in an increased cellular uptake of the particles. The lip-MNPs were able to transfect the RBE4 cells with an incidence of approximately 20% of a commercial transfection agent. The in vivo distribution studies revealed that lip-MNPs had superior pharmacokinetic properties due to evasion of the RES, including hepatic Kuppfer cells and macrophages in the spleen. In conclusion, we were able to design a novel lipid-encapsulated MNP with the ability to carry genetic material, with favorable pharmacokinetic properties, and under the influence of a magnetic field with the capability to mediate transfection in vitro. |
format | Online Article Text |
id | pubmed-3834948 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-38349482013-11-21 Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery Linemann, Thomas Thomsen, Louiza B. Du Jardin, Kristian G. Laursen, Jens C. Jensen, Jesper B. Lichota, Jacek Moos, Torben Pharmaceutics Article The aim of the present study was to evaluate the transfection potential of chitosan-coated, green-fluorescent magnetic nanoparticles (MNPs) (chi-MNPs) after encapsulation inside polyethylglycol (PEG)ylated liposomes that produced lipid-encapsulated chitosan-coated MNPs (lip-MNPs), and also to evaluate how these particles would distribute in vivo after systemic injection. The transfection potential of both chi-MNPs and lip-MNPs was evaluated in vitro in rat brain endothelial 4 (RBE4) cells with and without applying a magnetic field. Subsequently, the MNPs were evaluated in vivo in young rats. The in vitro investigations revealed that the application of a magnetic field resulted in an increased cellular uptake of the particles. The lip-MNPs were able to transfect the RBE4 cells with an incidence of approximately 20% of a commercial transfection agent. The in vivo distribution studies revealed that lip-MNPs had superior pharmacokinetic properties due to evasion of the RES, including hepatic Kuppfer cells and macrophages in the spleen. In conclusion, we were able to design a novel lipid-encapsulated MNP with the ability to carry genetic material, with favorable pharmacokinetic properties, and under the influence of a magnetic field with the capability to mediate transfection in vitro. MDPI 2013-04-23 /pmc/articles/PMC3834948/ /pubmed/24300449 http://dx.doi.org/10.3390/pharmaceutics5020246 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Linemann, Thomas Thomsen, Louiza B. Du Jardin, Kristian G. Laursen, Jens C. Jensen, Jesper B. Lichota, Jacek Moos, Torben Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery |
title | Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery |
title_full | Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery |
title_fullStr | Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery |
title_full_unstemmed | Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery |
title_short | Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery |
title_sort | development of a novel lipophilic, magnetic nanoparticle for in vivo drug delivery |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834948/ https://www.ncbi.nlm.nih.gov/pubmed/24300449 http://dx.doi.org/10.3390/pharmaceutics5020246 |
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