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Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin

In Drosophila melanogaster M. (Diptera: Drosophilidae), a phospholipase-C to proteininase-C signal cascade leads to the endocytic uptake of yolk precursor molecules. The data suggest that D. melanogaster has a phospholipase-C/proteinkinase-C signaling pathway similar to that previously shown to be r...

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Detalles Bibliográficos
Autores principales: Brubaker-Purkey, Bethany J., Woodruff, Richard I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: University of Wisconsin Library 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835028/
https://www.ncbi.nlm.nih.gov/pubmed/24228869
http://dx.doi.org/10.1673/031.013.6801
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author Brubaker-Purkey, Bethany J.
Woodruff, Richard I.
author_facet Brubaker-Purkey, Bethany J.
Woodruff, Richard I.
author_sort Brubaker-Purkey, Bethany J.
collection PubMed
description In Drosophila melanogaster M. (Diptera: Drosophilidae), a phospholipase-C to proteininase-C signal cascade leads to the endocytic uptake of yolk precursor molecules. The data suggest that D. melanogaster has a phospholipase-C/proteinkinase-C signaling pathway similar to that previously shown to be required for vitellogenesis in the milkweed bug, Oncopeltus fasciatus Dallas (Hemiptera: Lygaeidae). Calmodulin, derived from epithelial cells and transported to the oocytes via gap junctions, may trigger this pathway. To investigate this, a series of known antagonists to various elements of the pathway were used. W-7 (which prevents calmodulin binding to phospholipase-C), U-73122 (which prevents activation of phospholipase-C), verapamil (which blocks Ca(2+) release by IP3), HAG (which blocks diacylglycerol), and staurosporine (which inactivates proteinkinase-C) were each shown to inhibit endocytosis, thereby blocking formation of nascent yolk spheres.
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spelling pubmed-38350282013-11-26 Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin Brubaker-Purkey, Bethany J. Woodruff, Richard I. J Insect Sci Article In Drosophila melanogaster M. (Diptera: Drosophilidae), a phospholipase-C to proteininase-C signal cascade leads to the endocytic uptake of yolk precursor molecules. The data suggest that D. melanogaster has a phospholipase-C/proteinkinase-C signaling pathway similar to that previously shown to be required for vitellogenesis in the milkweed bug, Oncopeltus fasciatus Dallas (Hemiptera: Lygaeidae). Calmodulin, derived from epithelial cells and transported to the oocytes via gap junctions, may trigger this pathway. To investigate this, a series of known antagonists to various elements of the pathway were used. W-7 (which prevents calmodulin binding to phospholipase-C), U-73122 (which prevents activation of phospholipase-C), verapamil (which blocks Ca(2+) release by IP3), HAG (which blocks diacylglycerol), and staurosporine (which inactivates proteinkinase-C) were each shown to inhibit endocytosis, thereby blocking formation of nascent yolk spheres. University of Wisconsin Library 2013-07-15 /pmc/articles/PMC3835028/ /pubmed/24228869 http://dx.doi.org/10.1673/031.013.6801 Text en © 2013 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Brubaker-Purkey, Bethany J.
Woodruff, Richard I.
Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin
title Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin
title_full Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin
title_fullStr Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin
title_full_unstemmed Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin
title_short Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin
title_sort vitellogenesis in the fruit fly, drosophila melanogaster: antagonists demonstrate that the plc, ip(3)/dag, pk-c pathway is triggered by calmodulin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835028/
https://www.ncbi.nlm.nih.gov/pubmed/24228869
http://dx.doi.org/10.1673/031.013.6801
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