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Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin
In Drosophila melanogaster M. (Diptera: Drosophilidae), a phospholipase-C to proteininase-C signal cascade leads to the endocytic uptake of yolk precursor molecules. The data suggest that D. melanogaster has a phospholipase-C/proteinkinase-C signaling pathway similar to that previously shown to be r...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
University of Wisconsin Library
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835028/ https://www.ncbi.nlm.nih.gov/pubmed/24228869 http://dx.doi.org/10.1673/031.013.6801 |
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author | Brubaker-Purkey, Bethany J. Woodruff, Richard I. |
author_facet | Brubaker-Purkey, Bethany J. Woodruff, Richard I. |
author_sort | Brubaker-Purkey, Bethany J. |
collection | PubMed |
description | In Drosophila melanogaster M. (Diptera: Drosophilidae), a phospholipase-C to proteininase-C signal cascade leads to the endocytic uptake of yolk precursor molecules. The data suggest that D. melanogaster has a phospholipase-C/proteinkinase-C signaling pathway similar to that previously shown to be required for vitellogenesis in the milkweed bug, Oncopeltus fasciatus Dallas (Hemiptera: Lygaeidae). Calmodulin, derived from epithelial cells and transported to the oocytes via gap junctions, may trigger this pathway. To investigate this, a series of known antagonists to various elements of the pathway were used. W-7 (which prevents calmodulin binding to phospholipase-C), U-73122 (which prevents activation of phospholipase-C), verapamil (which blocks Ca(2+) release by IP3), HAG (which blocks diacylglycerol), and staurosporine (which inactivates proteinkinase-C) were each shown to inhibit endocytosis, thereby blocking formation of nascent yolk spheres. |
format | Online Article Text |
id | pubmed-3835028 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | University of Wisconsin Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-38350282013-11-26 Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin Brubaker-Purkey, Bethany J. Woodruff, Richard I. J Insect Sci Article In Drosophila melanogaster M. (Diptera: Drosophilidae), a phospholipase-C to proteininase-C signal cascade leads to the endocytic uptake of yolk precursor molecules. The data suggest that D. melanogaster has a phospholipase-C/proteinkinase-C signaling pathway similar to that previously shown to be required for vitellogenesis in the milkweed bug, Oncopeltus fasciatus Dallas (Hemiptera: Lygaeidae). Calmodulin, derived from epithelial cells and transported to the oocytes via gap junctions, may trigger this pathway. To investigate this, a series of known antagonists to various elements of the pathway were used. W-7 (which prevents calmodulin binding to phospholipase-C), U-73122 (which prevents activation of phospholipase-C), verapamil (which blocks Ca(2+) release by IP3), HAG (which blocks diacylglycerol), and staurosporine (which inactivates proteinkinase-C) were each shown to inhibit endocytosis, thereby blocking formation of nascent yolk spheres. University of Wisconsin Library 2013-07-15 /pmc/articles/PMC3835028/ /pubmed/24228869 http://dx.doi.org/10.1673/031.013.6801 Text en © 2013 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Brubaker-Purkey, Bethany J. Woodruff, Richard I. Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin |
title | Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin |
title_full | Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin |
title_fullStr | Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin |
title_full_unstemmed | Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin |
title_short | Vitellogenesis in the Fruit Fly, Drosophila melanogaster: Antagonists Demonstrate that the PLC, IP(3)/DAG, PK-C Pathway is Triggered by Calmodulin |
title_sort | vitellogenesis in the fruit fly, drosophila melanogaster: antagonists demonstrate that the plc, ip(3)/dag, pk-c pathway is triggered by calmodulin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835028/ https://www.ncbi.nlm.nih.gov/pubmed/24228869 http://dx.doi.org/10.1673/031.013.6801 |
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