Cargando…

Prolonged Postdiapause: Influence on some Indicators of Carbohydrate and Lipid Metabolism of the Red Mason Bee, Osmia rufa

Bees of the genus Osmia are being used in crop pollination at an increasing rate. However, a short life expectancy of adult individuals limits the feasibility of their use. Cocoons of the red mason bee, Osmia rufa L. (Hymenoptera: Megachilidae), can be stored at 4° C in a postdiapause state, and adu...

Descripción completa

Detalles Bibliográficos
Autores principales: Dmochowska, Kamila, Giejdasz, Karol, Fliszkiewicz, Monika, Żółtowska, Krystyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: University of Wisconsin Library 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835046/
https://www.ncbi.nlm.nih.gov/pubmed/24219557
http://dx.doi.org/10.1673/031.013.7701
_version_ 1782292088503664640
author Dmochowska, Kamila
Giejdasz, Karol
Fliszkiewicz, Monika
Żółtowska, Krystyna
author_facet Dmochowska, Kamila
Giejdasz, Karol
Fliszkiewicz, Monika
Żółtowska, Krystyna
author_sort Dmochowska, Kamila
collection PubMed
description Bees of the genus Osmia are being used in crop pollination at an increasing rate. However, a short life expectancy of adult individuals limits the feasibility of their use. Cocoons of the red mason bee, Osmia rufa L. (Hymenoptera: Megachilidae), can be stored at 4° C in a postdiapause state, and adult bees can be used for pollination outside their natural flight period. The period of storage in this form has an unfavorable influence on the survival rate, life expectancy, and fertility of the bee. It was suggested that the negative results are connected with exhaustion of energy reserves. To test this hypothesis, the present study examined the contents of protein, carbohydrates, lipids, and the activities of some enzymes, and their degradation in red mason bees that emerged in spring according to their biological clock and in summer after elongated diapause. It was found that postdiapause artificially elongated by 3 months caused significant decreases in body weight, total sugar, glycogen, lipids, and protein content in O. rufa. Glucose level was highest in bees that emerged in the summer, which was coincident with increased activities of maltase and trehalase. The activities of sucrase and cellobiase were not changed, while amylase activity was considerably decreased. The activities of triacylglycerols lipase and C2, C4, C10 carboxylesterases were highest in bees that emerged in July. Low temperatures restrict O. rufa emergence, and during prolonged postdiapause, metabolic processes lead to significant reductions of structural and energetic compounds.
format Online
Article
Text
id pubmed-3835046
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher University of Wisconsin Library
record_format MEDLINE/PubMed
spelling pubmed-38350462013-11-26 Prolonged Postdiapause: Influence on some Indicators of Carbohydrate and Lipid Metabolism of the Red Mason Bee, Osmia rufa Dmochowska, Kamila Giejdasz, Karol Fliszkiewicz, Monika Żółtowska, Krystyna J Insect Sci Article Bees of the genus Osmia are being used in crop pollination at an increasing rate. However, a short life expectancy of adult individuals limits the feasibility of their use. Cocoons of the red mason bee, Osmia rufa L. (Hymenoptera: Megachilidae), can be stored at 4° C in a postdiapause state, and adult bees can be used for pollination outside their natural flight period. The period of storage in this form has an unfavorable influence on the survival rate, life expectancy, and fertility of the bee. It was suggested that the negative results are connected with exhaustion of energy reserves. To test this hypothesis, the present study examined the contents of protein, carbohydrates, lipids, and the activities of some enzymes, and their degradation in red mason bees that emerged in spring according to their biological clock and in summer after elongated diapause. It was found that postdiapause artificially elongated by 3 months caused significant decreases in body weight, total sugar, glycogen, lipids, and protein content in O. rufa. Glucose level was highest in bees that emerged in the summer, which was coincident with increased activities of maltase and trehalase. The activities of sucrase and cellobiase were not changed, while amylase activity was considerably decreased. The activities of triacylglycerols lipase and C2, C4, C10 carboxylesterases were highest in bees that emerged in July. Low temperatures restrict O. rufa emergence, and during prolonged postdiapause, metabolic processes lead to significant reductions of structural and energetic compounds. University of Wisconsin Library 2013-08-10 /pmc/articles/PMC3835046/ /pubmed/24219557 http://dx.doi.org/10.1673/031.013.7701 Text en © 2013 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Dmochowska, Kamila
Giejdasz, Karol
Fliszkiewicz, Monika
Żółtowska, Krystyna
Prolonged Postdiapause: Influence on some Indicators of Carbohydrate and Lipid Metabolism of the Red Mason Bee, Osmia rufa
title Prolonged Postdiapause: Influence on some Indicators of Carbohydrate and Lipid Metabolism of the Red Mason Bee, Osmia rufa
title_full Prolonged Postdiapause: Influence on some Indicators of Carbohydrate and Lipid Metabolism of the Red Mason Bee, Osmia rufa
title_fullStr Prolonged Postdiapause: Influence on some Indicators of Carbohydrate and Lipid Metabolism of the Red Mason Bee, Osmia rufa
title_full_unstemmed Prolonged Postdiapause: Influence on some Indicators of Carbohydrate and Lipid Metabolism of the Red Mason Bee, Osmia rufa
title_short Prolonged Postdiapause: Influence on some Indicators of Carbohydrate and Lipid Metabolism of the Red Mason Bee, Osmia rufa
title_sort prolonged postdiapause: influence on some indicators of carbohydrate and lipid metabolism of the red mason bee, osmia rufa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835046/
https://www.ncbi.nlm.nih.gov/pubmed/24219557
http://dx.doi.org/10.1673/031.013.7701
work_keys_str_mv AT dmochowskakamila prolongedpostdiapauseinfluenceonsomeindicatorsofcarbohydrateandlipidmetabolismoftheredmasonbeeosmiarufa
AT giejdaszkarol prolongedpostdiapauseinfluenceonsomeindicatorsofcarbohydrateandlipidmetabolismoftheredmasonbeeosmiarufa
AT fliszkiewiczmonika prolongedpostdiapauseinfluenceonsomeindicatorsofcarbohydrateandlipidmetabolismoftheredmasonbeeosmiarufa
AT zołtowskakrystyna prolongedpostdiapauseinfluenceonsomeindicatorsofcarbohydrateandlipidmetabolismoftheredmasonbeeosmiarufa