Cargando…
Ellagic Acid Reduces Adipogenesis through Inhibition of Differentiation-Prevention of the Induction of Rb Phosphorylation in 3T3-L1 Adipocytes
Ellagic acid (EA) present in many fruits and nuts serves as antiproliferation, anti-inflammatory, and antitumorigenic properties. However, the effect of EA on preadipocytes adipogenesis and its mechanism(s) have not been elucidated. The present study was designed to examine the effect of EA on adipo...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835195/ https://www.ncbi.nlm.nih.gov/pubmed/24302962 http://dx.doi.org/10.1155/2013/287534 |
_version_ | 1782292113566728192 |
---|---|
author | Wang, Lifeng Li, Linlin Ran, Xinjian Long, Mei Zhang, Minfang Tao, Yicun Luo, Xin Wang, Ye Ma, Xiaoli Halmurati, Upur Mao, Xinmin Ren, Jun |
author_facet | Wang, Lifeng Li, Linlin Ran, Xinjian Long, Mei Zhang, Minfang Tao, Yicun Luo, Xin Wang, Ye Ma, Xiaoli Halmurati, Upur Mao, Xinmin Ren, Jun |
author_sort | Wang, Lifeng |
collection | PubMed |
description | Ellagic acid (EA) present in many fruits and nuts serves as antiproliferation, anti-inflammatory, and antitumorigenic properties. However, the effect of EA on preadipocytes adipogenesis and its mechanism(s) have not been elucidated. The present study was designed to examine the effect of EA on adipogenesis in 3T3-L1 preadipocytes and underlying mechanism(s) of action involved. Data show that EA administration decreased the accumulation of lipid droplets. The inhibition was diminished when the addition of EA was delayed to days 2–4 of differentiation. Clonal expansion was reduced in the presence of EA. FACS analysis showed that EA blocked the cell cycle at the G1/S transition. EdU incorporation also confirmed that EA refrained cell from entering S phase. Our data also revealed that the differentiation-induced protein expression of Cyclin A and phosphorylation of the retinoblastoma protein (Rb) were impaired by EA. Differentiation-dependent expression and DNA-binding ability of C/EBPα were also inhibited by EA. Alterations in cell cycle-associated proteins may be important with respect to the antiadipogenic action of EA. In conclusion, EA is capable of inhibiting adipogenesis in 3T3-L1 adipocytes possibly through reduction of Cyclin A protein expression and Rb phosphorylation. With the blocking of G1/S phase transition, EA suppresses terminal differentiation and lipid accumulation in 3T3-L1 adipocytes. |
format | Online Article Text |
id | pubmed-3835195 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38351952013-12-03 Ellagic Acid Reduces Adipogenesis through Inhibition of Differentiation-Prevention of the Induction of Rb Phosphorylation in 3T3-L1 Adipocytes Wang, Lifeng Li, Linlin Ran, Xinjian Long, Mei Zhang, Minfang Tao, Yicun Luo, Xin Wang, Ye Ma, Xiaoli Halmurati, Upur Mao, Xinmin Ren, Jun Evid Based Complement Alternat Med Research Article Ellagic acid (EA) present in many fruits and nuts serves as antiproliferation, anti-inflammatory, and antitumorigenic properties. However, the effect of EA on preadipocytes adipogenesis and its mechanism(s) have not been elucidated. The present study was designed to examine the effect of EA on adipogenesis in 3T3-L1 preadipocytes and underlying mechanism(s) of action involved. Data show that EA administration decreased the accumulation of lipid droplets. The inhibition was diminished when the addition of EA was delayed to days 2–4 of differentiation. Clonal expansion was reduced in the presence of EA. FACS analysis showed that EA blocked the cell cycle at the G1/S transition. EdU incorporation also confirmed that EA refrained cell from entering S phase. Our data also revealed that the differentiation-induced protein expression of Cyclin A and phosphorylation of the retinoblastoma protein (Rb) were impaired by EA. Differentiation-dependent expression and DNA-binding ability of C/EBPα were also inhibited by EA. Alterations in cell cycle-associated proteins may be important with respect to the antiadipogenic action of EA. In conclusion, EA is capable of inhibiting adipogenesis in 3T3-L1 adipocytes possibly through reduction of Cyclin A protein expression and Rb phosphorylation. With the blocking of G1/S phase transition, EA suppresses terminal differentiation and lipid accumulation in 3T3-L1 adipocytes. Hindawi Publishing Corporation 2013 2013-11-04 /pmc/articles/PMC3835195/ /pubmed/24302962 http://dx.doi.org/10.1155/2013/287534 Text en Copyright © 2013 Lifeng Wang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Lifeng Li, Linlin Ran, Xinjian Long, Mei Zhang, Minfang Tao, Yicun Luo, Xin Wang, Ye Ma, Xiaoli Halmurati, Upur Mao, Xinmin Ren, Jun Ellagic Acid Reduces Adipogenesis through Inhibition of Differentiation-Prevention of the Induction of Rb Phosphorylation in 3T3-L1 Adipocytes |
title | Ellagic Acid Reduces Adipogenesis through Inhibition of Differentiation-Prevention of the Induction of Rb Phosphorylation in 3T3-L1 Adipocytes |
title_full | Ellagic Acid Reduces Adipogenesis through Inhibition of Differentiation-Prevention of the Induction of Rb Phosphorylation in 3T3-L1 Adipocytes |
title_fullStr | Ellagic Acid Reduces Adipogenesis through Inhibition of Differentiation-Prevention of the Induction of Rb Phosphorylation in 3T3-L1 Adipocytes |
title_full_unstemmed | Ellagic Acid Reduces Adipogenesis through Inhibition of Differentiation-Prevention of the Induction of Rb Phosphorylation in 3T3-L1 Adipocytes |
title_short | Ellagic Acid Reduces Adipogenesis through Inhibition of Differentiation-Prevention of the Induction of Rb Phosphorylation in 3T3-L1 Adipocytes |
title_sort | ellagic acid reduces adipogenesis through inhibition of differentiation-prevention of the induction of rb phosphorylation in 3t3-l1 adipocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835195/ https://www.ncbi.nlm.nih.gov/pubmed/24302962 http://dx.doi.org/10.1155/2013/287534 |
work_keys_str_mv | AT wanglifeng ellagicacidreducesadipogenesisthroughinhibitionofdifferentiationpreventionoftheinductionofrbphosphorylationin3t3l1adipocytes AT lilinlin ellagicacidreducesadipogenesisthroughinhibitionofdifferentiationpreventionoftheinductionofrbphosphorylationin3t3l1adipocytes AT ranxinjian ellagicacidreducesadipogenesisthroughinhibitionofdifferentiationpreventionoftheinductionofrbphosphorylationin3t3l1adipocytes AT longmei ellagicacidreducesadipogenesisthroughinhibitionofdifferentiationpreventionoftheinductionofrbphosphorylationin3t3l1adipocytes AT zhangminfang ellagicacidreducesadipogenesisthroughinhibitionofdifferentiationpreventionoftheinductionofrbphosphorylationin3t3l1adipocytes AT taoyicun ellagicacidreducesadipogenesisthroughinhibitionofdifferentiationpreventionoftheinductionofrbphosphorylationin3t3l1adipocytes AT luoxin ellagicacidreducesadipogenesisthroughinhibitionofdifferentiationpreventionoftheinductionofrbphosphorylationin3t3l1adipocytes AT wangye ellagicacidreducesadipogenesisthroughinhibitionofdifferentiationpreventionoftheinductionofrbphosphorylationin3t3l1adipocytes AT maxiaoli ellagicacidreducesadipogenesisthroughinhibitionofdifferentiationpreventionoftheinductionofrbphosphorylationin3t3l1adipocytes AT halmuratiupur ellagicacidreducesadipogenesisthroughinhibitionofdifferentiationpreventionoftheinductionofrbphosphorylationin3t3l1adipocytes AT maoxinmin ellagicacidreducesadipogenesisthroughinhibitionofdifferentiationpreventionoftheinductionofrbphosphorylationin3t3l1adipocytes AT renjun ellagicacidreducesadipogenesisthroughinhibitionofdifferentiationpreventionoftheinductionofrbphosphorylationin3t3l1adipocytes |