Accumulated lipids rather than the rigid cell walls impede the extraction of genetic materials for effective colony PCRs in Chlorella vulgaris

BACKGROUND: Failure of colony PCRs in green microalga Chlorella vulgaris is typically attributed to the difficulty in disrupting its notoriously rigid cell walls for releasing the genetic materials and therefore the development of an effective colony PCR procedure in C. vulgaris presents a challenge...

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Detalles Bibliográficos
Autores principales: Tear, Crystal Jing Ying, Lim, Chanyuen, Wu, Jinchuan, Zhao, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835426/
https://www.ncbi.nlm.nih.gov/pubmed/24219401
http://dx.doi.org/10.1186/1475-2859-12-106

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835426/
https://www.ncbi.nlm.nih.gov/pubmed/24219401
http://dx.doi.org/10.1186/1475-2859-12-106

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