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Accumulated lipids rather than the rigid cell walls impede the extraction of genetic materials for effective colony PCRs in Chlorella vulgaris
BACKGROUND: Failure of colony PCRs in green microalga Chlorella vulgaris is typically attributed to the difficulty in disrupting its notoriously rigid cell walls for releasing the genetic materials and therefore the development of an effective colony PCR procedure in C. vulgaris presents a challenge...
Autores principales: | Tear, Crystal Jing Ying, Lim, Chanyuen, Wu, Jinchuan, Zhao, Hua |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835426/ https://www.ncbi.nlm.nih.gov/pubmed/24219401 http://dx.doi.org/10.1186/1475-2859-12-106 |
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