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Aqueous Extract of Annona macroprophyllata: A Potential α-Glucosidase Inhibitor
Annona genus contains plants used in folk medicine for the treatment of diabetes. In the present study, an aqueous extract prepared from Annona macroprophyllata (Annonaceae, also known as A. diversifolia) leaves was evaluated on both the activity of yeast α-glucosidase (an in vitro assay) and sucros...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835905/ https://www.ncbi.nlm.nih.gov/pubmed/24298552 http://dx.doi.org/10.1155/2013/591313 |
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author | Brindis, F. González-Trujano, M. E. González-Andrade, M. Aguirre-Hernández, E. Villalobos-Molina, R. |
author_facet | Brindis, F. González-Trujano, M. E. González-Andrade, M. Aguirre-Hernández, E. Villalobos-Molina, R. |
author_sort | Brindis, F. |
collection | PubMed |
description | Annona genus contains plants used in folk medicine for the treatment of diabetes. In the present study, an aqueous extract prepared from Annona macroprophyllata (Annonaceae, also known as A. diversifolia) leaves was evaluated on both the activity of yeast α-glucosidase (an in vitro assay) and sucrose tolerance in Wistar rats. The results have shown that the aqueous extract from A. macroprophyllata inhibits the yeast α-glucosidase with an IC(50) = 1.18 mg/mL, in a competitive manner with a K (i) = 0.97 mg/mL, a similar value to that of acarbose (K (i) = 0.79 mg/mL). The inhibitory activity of A. macroprophyllata was reinforced by its antihyperglycemic effect, at doses of 100, 300, and 500 mg/kg in rats. Chromatographic analysis identified the flavonoids rutin and isoquercitrin in the most polar fractions of A. macroprophyllata crude extract, suggesting that these flavonoids are part of the active constituents in the plant. Our results support the use of A. macroprophyllata in Mexican folk medicine to control postprandial glycemia in people with diabetes mellitus, involving active constituents of flavonoid nature. |
format | Online Article Text |
id | pubmed-3835905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38359052013-12-02 Aqueous Extract of Annona macroprophyllata: A Potential α-Glucosidase Inhibitor Brindis, F. González-Trujano, M. E. González-Andrade, M. Aguirre-Hernández, E. Villalobos-Molina, R. Biomed Res Int Research Article Annona genus contains plants used in folk medicine for the treatment of diabetes. In the present study, an aqueous extract prepared from Annona macroprophyllata (Annonaceae, also known as A. diversifolia) leaves was evaluated on both the activity of yeast α-glucosidase (an in vitro assay) and sucrose tolerance in Wistar rats. The results have shown that the aqueous extract from A. macroprophyllata inhibits the yeast α-glucosidase with an IC(50) = 1.18 mg/mL, in a competitive manner with a K (i) = 0.97 mg/mL, a similar value to that of acarbose (K (i) = 0.79 mg/mL). The inhibitory activity of A. macroprophyllata was reinforced by its antihyperglycemic effect, at doses of 100, 300, and 500 mg/kg in rats. Chromatographic analysis identified the flavonoids rutin and isoquercitrin in the most polar fractions of A. macroprophyllata crude extract, suggesting that these flavonoids are part of the active constituents in the plant. Our results support the use of A. macroprophyllata in Mexican folk medicine to control postprandial glycemia in people with diabetes mellitus, involving active constituents of flavonoid nature. Hindawi Publishing Corporation 2013 2013-11-04 /pmc/articles/PMC3835905/ /pubmed/24298552 http://dx.doi.org/10.1155/2013/591313 Text en Copyright © 2013 F. Brindis et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Brindis, F. González-Trujano, M. E. González-Andrade, M. Aguirre-Hernández, E. Villalobos-Molina, R. Aqueous Extract of Annona macroprophyllata: A Potential α-Glucosidase Inhibitor |
title | Aqueous Extract of Annona macroprophyllata: A Potential α-Glucosidase Inhibitor |
title_full | Aqueous Extract of Annona macroprophyllata: A Potential α-Glucosidase Inhibitor |
title_fullStr | Aqueous Extract of Annona macroprophyllata: A Potential α-Glucosidase Inhibitor |
title_full_unstemmed | Aqueous Extract of Annona macroprophyllata: A Potential α-Glucosidase Inhibitor |
title_short | Aqueous Extract of Annona macroprophyllata: A Potential α-Glucosidase Inhibitor |
title_sort | aqueous extract of annona macroprophyllata: a potential α-glucosidase inhibitor |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3835905/ https://www.ncbi.nlm.nih.gov/pubmed/24298552 http://dx.doi.org/10.1155/2013/591313 |
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