Poor Glycemic Control Is Related to Increased Nitric Oxide Activity Within the Renal Circulation of Patients With Type 2 Diabetes

OBJECTIVE: Experimental studies have shown that glucose releases endothelial nitric oxide (NO) and that NO contributes to renal hyperperfusion in models of diabetes. To examine whether this translates into the human condition, we studied the relationship between glycemic control and renal NO activity in patients with type 2 diabetes. RESEARCH DESIGN AND METHODS: A total of 113 patients with type 2 diabetes and a wide range of HbA(1c) concentrations were included. Renal plasma flow (RPF) and glomerular filtration rate (GFR) were determined by constant infusion input clearance. Functional NO activity in the renal circulation was determined as change of RPF to infusion of the NO synthase (NOS) inhibitor N(G)-monomethyl-l-arginine (l-NMMA) (4.25 mg/kg). As additional markers, we measured urinary excretion of NO (UNOx) and l-arginine–to–asymmetrical dimethylarginine (ADMA) ratio in plasma. RESULTS: Subjects within the highest tertile of HbA(1c) concentration had increased RPF (low, medium, and high tertiles 576 ± 17 vs. 585 ± 22 vs. 627 ± 33 mL/min/m(2), P = 0.05 by one-way ANOVA), while GFR was similar across tertiles. The response of RPF to NOS blockade was augmented in subjects with higher HbA(1c) levels (−55 ± 7 vs. −64 ± 8 vs. −86 ± 8 mL/min, P = 0.04 by one-way ANOVA). Further, l-arginine–to–ADMA ratio and UNOx were increased in subjects with higher HbA(1c) levels. CONCLUSIONS: In line with experimental evidence, we could demonstrate in humans that poor glycemic control is related to higher NO activity and hyperperfusion of the kidney. The renal NO system may thus be a novel therapeutic target for improving renal hemodynamics in patients with diabetes.