Construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases()

Neuraminidase (NA) is a surface glycoprotein produced by the influenza virus. Specific NA mutations that confer resistance to anti-viral drugs have been reported. The aim of this study was to demonstrate quick preparation of the mutated NAs using the yeast surface display and its applicability for s...

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Autores principales: Shigemori, Tomohiro, Nagayama, Mitsuru, Yamada, Junki, Miura, Natsuko, Yongkiettrakul, Suganya, Kuroda, Kouichi, Katsuragi, Tohoru, Ueda, Mitsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3836197/
https://www.ncbi.nlm.nih.gov/pubmed/24265981
http://dx.doi.org/10.1016/j.fob.2013.10.007
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author Shigemori, Tomohiro
Nagayama, Mitsuru
Yamada, Junki
Miura, Natsuko
Yongkiettrakul, Suganya
Kuroda, Kouichi
Katsuragi, Tohoru
Ueda, Mitsuyoshi
author_facet Shigemori, Tomohiro
Nagayama, Mitsuru
Yamada, Junki
Miura, Natsuko
Yongkiettrakul, Suganya
Kuroda, Kouichi
Katsuragi, Tohoru
Ueda, Mitsuyoshi
author_sort Shigemori, Tomohiro
collection PubMed
description Neuraminidase (NA) is a surface glycoprotein produced by the influenza virus. Specific NA mutations that confer resistance to anti-viral drugs have been reported. The aim of this study was to demonstrate quick preparation of the mutated NAs using the yeast surface display and its applicability for screening inhibitors. Plasmids encoding the head domain of wild-type and drug-resistant NAs were constructed and introduced into yeast, and these were successfully displayed on the yeast surface, with biochemical properties similar to the native virus NAs. This system using mutated NAs-displaying yeast provides an efficient and convenient tool for screening novel inhibitors against the drug-resistant influenza virus.
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spelling pubmed-38361972013-11-21 Construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases() Shigemori, Tomohiro Nagayama, Mitsuru Yamada, Junki Miura, Natsuko Yongkiettrakul, Suganya Kuroda, Kouichi Katsuragi, Tohoru Ueda, Mitsuyoshi FEBS Open Bio Article Neuraminidase (NA) is a surface glycoprotein produced by the influenza virus. Specific NA mutations that confer resistance to anti-viral drugs have been reported. The aim of this study was to demonstrate quick preparation of the mutated NAs using the yeast surface display and its applicability for screening inhibitors. Plasmids encoding the head domain of wild-type and drug-resistant NAs were constructed and introduced into yeast, and these were successfully displayed on the yeast surface, with biochemical properties similar to the native virus NAs. This system using mutated NAs-displaying yeast provides an efficient and convenient tool for screening novel inhibitors against the drug-resistant influenza virus. Elsevier 2013-10-29 /pmc/articles/PMC3836197/ /pubmed/24265981 http://dx.doi.org/10.1016/j.fob.2013.10.007 Text en © 2013 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Shigemori, Tomohiro
Nagayama, Mitsuru
Yamada, Junki
Miura, Natsuko
Yongkiettrakul, Suganya
Kuroda, Kouichi
Katsuragi, Tohoru
Ueda, Mitsuyoshi
Construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases()
title Construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases()
title_full Construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases()
title_fullStr Construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases()
title_full_unstemmed Construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases()
title_short Construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases()
title_sort construction of a convenient system for easily screening inhibitors of mutated influenza virus neuraminidases()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3836197/
https://www.ncbi.nlm.nih.gov/pubmed/24265981
http://dx.doi.org/10.1016/j.fob.2013.10.007
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