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iTRAQ-based proteomics reveals novel biomarkers of osteoarthritis
OBJECTIVE: We performed comprehensive proteomic analyses of articular cartilage by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and searched for candidate biomarkers for osteoarthritis (OA). METHODS: Articular cartilage was collected from patients with OA or femoral...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Informa UK Ltd.
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3836424/ https://www.ncbi.nlm.nih.gov/pubmed/23937207 http://dx.doi.org/10.3109/1354750X.2013.810667 |
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author | Ikeda, Daiki Ageta, Hiroshi Tsuchida, Kunihiro Yamada, Harumoto |
author_facet | Ikeda, Daiki Ageta, Hiroshi Tsuchida, Kunihiro Yamada, Harumoto |
author_sort | Ikeda, Daiki |
collection | PubMed |
description | OBJECTIVE: We performed comprehensive proteomic analyses of articular cartilage by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and searched for candidate biomarkers for osteoarthritis (OA). METHODS: Articular cartilage was collected from patients with OA or femoral neck fracture for the control group. Molecular variations were detected by the iTRAQ method, and quantitative analyses were performed by western blot. RESULTS: Using the iTRAQ method, we identified 76 proteins with different expression levels in OA patients and the control group. Among these proteins, we selected LECT2 (leukocyte cell-derived chemotaxin-2), BAALC (brain and acute leukemia, cytoplasmic), and PRDX6 (peroxiredoxin-6), which had not been reported as biomarkers for OA. CONCLUSIONS: Use of these proteins in combination with conventional OA biomarkers may better reflect the grade and prognosis of OA. |
format | Online Article Text |
id | pubmed-3836424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Informa UK Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-38364242013-11-22 iTRAQ-based proteomics reveals novel biomarkers of osteoarthritis Ikeda, Daiki Ageta, Hiroshi Tsuchida, Kunihiro Yamada, Harumoto Biomarkers Research Article OBJECTIVE: We performed comprehensive proteomic analyses of articular cartilage by using the isobaric tags for relative and absolute quantitation (iTRAQ) method, and searched for candidate biomarkers for osteoarthritis (OA). METHODS: Articular cartilage was collected from patients with OA or femoral neck fracture for the control group. Molecular variations were detected by the iTRAQ method, and quantitative analyses were performed by western blot. RESULTS: Using the iTRAQ method, we identified 76 proteins with different expression levels in OA patients and the control group. Among these proteins, we selected LECT2 (leukocyte cell-derived chemotaxin-2), BAALC (brain and acute leukemia, cytoplasmic), and PRDX6 (peroxiredoxin-6), which had not been reported as biomarkers for OA. CONCLUSIONS: Use of these proteins in combination with conventional OA biomarkers may better reflect the grade and prognosis of OA. Informa UK Ltd. 2013-11 2013-08-12 /pmc/articles/PMC3836424/ /pubmed/23937207 http://dx.doi.org/10.3109/1354750X.2013.810667 Text en © 2013 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the source is credited. |
spellingShingle | Research Article Ikeda, Daiki Ageta, Hiroshi Tsuchida, Kunihiro Yamada, Harumoto iTRAQ-based proteomics reveals novel biomarkers of osteoarthritis |
title | iTRAQ-based proteomics reveals novel biomarkers of osteoarthritis |
title_full | iTRAQ-based proteomics reveals novel biomarkers of osteoarthritis |
title_fullStr | iTRAQ-based proteomics reveals novel biomarkers of osteoarthritis |
title_full_unstemmed | iTRAQ-based proteomics reveals novel biomarkers of osteoarthritis |
title_short | iTRAQ-based proteomics reveals novel biomarkers of osteoarthritis |
title_sort | itraq-based proteomics reveals novel biomarkers of osteoarthritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3836424/ https://www.ncbi.nlm.nih.gov/pubmed/23937207 http://dx.doi.org/10.3109/1354750X.2013.810667 |
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