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The non-primate hepacivirus 5′ untranslated region possesses internal ribosomal entry site activity

The 5′ untranslated region (5′UTR) of the recently described non-primate hepacivirus (NPHV) contains a region with sequence homology to the internal ribosomal entry site (IRES) of hepatitis C virus (HCV) and GB virus B (GBV-B). Here, we demonstrated internal translation initiation by the NPHV 5′UTR...

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Autores principales: Stewart, Hazel, Walter, Cheryl, Jones, Dale, Lyons, Sinead, Simmonds, Peter, Harris, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for General Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3836496/
https://www.ncbi.nlm.nih.gov/pubmed/24026670
http://dx.doi.org/10.1099/vir.0.055764-0
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author Stewart, Hazel
Walter, Cheryl
Jones, Dale
Lyons, Sinead
Simmonds, Peter
Harris, Mark
author_facet Stewart, Hazel
Walter, Cheryl
Jones, Dale
Lyons, Sinead
Simmonds, Peter
Harris, Mark
author_sort Stewart, Hazel
collection PubMed
description The 5′ untranslated region (5′UTR) of the recently described non-primate hepacivirus (NPHV) contains a region with sequence homology to the internal ribosomal entry site (IRES) of hepatitis C virus (HCV) and GB virus B (GBV-B). Here, we demonstrated internal translation initiation by the NPHV 5′UTR in a bicistronic vector. An RNA stem–loop upstream of the NPHV IRES was structurally distinct from corresponding regions in HCV and GBV-B, and was not required for IRES function. Insertion of the NPHV stem–loop into the corresponding region of the HCV 5′UTR within the HCV subgenomic replicon significantly impaired RNA replication, indicating that long-range interactions between the 5′UTR and cis-acting downstream elements within the NPHV genome are not interchangeable with those of HCV. Despite similarities in IRES structure and function between hepaciviruses, replication elements in the NPHV 5′UTR appear functionally distinct from those of HCV.
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spelling pubmed-38364962013-12-04 The non-primate hepacivirus 5′ untranslated region possesses internal ribosomal entry site activity Stewart, Hazel Walter, Cheryl Jones, Dale Lyons, Sinead Simmonds, Peter Harris, Mark J Gen Virol Animal The 5′ untranslated region (5′UTR) of the recently described non-primate hepacivirus (NPHV) contains a region with sequence homology to the internal ribosomal entry site (IRES) of hepatitis C virus (HCV) and GB virus B (GBV-B). Here, we demonstrated internal translation initiation by the NPHV 5′UTR in a bicistronic vector. An RNA stem–loop upstream of the NPHV IRES was structurally distinct from corresponding regions in HCV and GBV-B, and was not required for IRES function. Insertion of the NPHV stem–loop into the corresponding region of the HCV 5′UTR within the HCV subgenomic replicon significantly impaired RNA replication, indicating that long-range interactions between the 5′UTR and cis-acting downstream elements within the NPHV genome are not interchangeable with those of HCV. Despite similarities in IRES structure and function between hepaciviruses, replication elements in the NPHV 5′UTR appear functionally distinct from those of HCV. Society for General Microbiology 2013-12 /pmc/articles/PMC3836496/ /pubmed/24026670 http://dx.doi.org/10.1099/vir.0.055764-0 Text en © 2013 SGM http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Animal
Stewart, Hazel
Walter, Cheryl
Jones, Dale
Lyons, Sinead
Simmonds, Peter
Harris, Mark
The non-primate hepacivirus 5′ untranslated region possesses internal ribosomal entry site activity
title The non-primate hepacivirus 5′ untranslated region possesses internal ribosomal entry site activity
title_full The non-primate hepacivirus 5′ untranslated region possesses internal ribosomal entry site activity
title_fullStr The non-primate hepacivirus 5′ untranslated region possesses internal ribosomal entry site activity
title_full_unstemmed The non-primate hepacivirus 5′ untranslated region possesses internal ribosomal entry site activity
title_short The non-primate hepacivirus 5′ untranslated region possesses internal ribosomal entry site activity
title_sort non-primate hepacivirus 5′ untranslated region possesses internal ribosomal entry site activity
topic Animal
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3836496/
https://www.ncbi.nlm.nih.gov/pubmed/24026670
http://dx.doi.org/10.1099/vir.0.055764-0
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