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Isolation of Saint Louis Encephalitis Virus from a Horse with Neurological Disease in Brazil

St. Louis encephalitis virus (SLEV) is a causative agent of encephalitis in humans in the Western hemisphere. SLEV is a positive-sense RNA virus that belongs to the Flavivirus genus, which includes West Nile encephalitis virus, Japanese encephalitis virus, Dengue virus and other medically important...

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Detalles Bibliográficos
Autores principales: Rosa, Roberta, Costa, Erica Azevedo, Marques, Rafael Elias, Oliveira, Taismara Simas, Furtini, Ronaldo, Bomfim, Maria Rosa Quaresma, Teixeira, Mauro Martins, Paixão, Tatiane Alves, Santos, Renato Lima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3836713/
https://www.ncbi.nlm.nih.gov/pubmed/24278489
http://dx.doi.org/10.1371/journal.pntd.0002537
Descripción
Sumario:St. Louis encephalitis virus (SLEV) is a causative agent of encephalitis in humans in the Western hemisphere. SLEV is a positive-sense RNA virus that belongs to the Flavivirus genus, which includes West Nile encephalitis virus, Japanese encephalitis virus, Dengue virus and other medically important viruses. Recently, we isolated a SLEV strain from the brain of a horse with neurological signs in the countryside of Minas Gerais, Brazil. The SLEV isolation was confirmed by reverse-transcription RT-PCR and sequencing of the E protein gene. Virus identity was also confirmed by indirect immunofluorescence using commercial antibodies against SLEV. To characterize this newly isolated strain in vivo, serial passages in newborn mice were performed and led to hemorrhagic manifestations associated with recruitment of inflammatory cells into the central nervous system of newborns. In summary this is the first isolation of SLEV from a horse with neurological signs in Brazil.