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Enzymatic Characterization of Insecticide Resistance Mechanisms in Field Populations of Malaysian Culex quinquefasciatus Say (Diptera: Culicidae)
BACKGROUND: There has been no comprehensive study on biochemical characterization of insecticide resistance mechanisms in field populations of Malaysian Culex quinquefasciatus. To fill this void in the literature, a nationwide investigation was performed to quantify the enzyme activities, thereby at...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3836847/ https://www.ncbi.nlm.nih.gov/pubmed/24278220 http://dx.doi.org/10.1371/journal.pone.0079928 |
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author | Low, Van Lun Chen, Chee Dhang Lee, Han Lim Tan, Tiong Kai Chen, Chin Fong Leong, Cherng Shii Lim, Yvonne Ai Lian Lim, Phaik Eem Norma-Rashid, Yusoff Sofian-Azirun, Mohd |
author_facet | Low, Van Lun Chen, Chee Dhang Lee, Han Lim Tan, Tiong Kai Chen, Chin Fong Leong, Cherng Shii Lim, Yvonne Ai Lian Lim, Phaik Eem Norma-Rashid, Yusoff Sofian-Azirun, Mohd |
author_sort | Low, Van Lun |
collection | PubMed |
description | BACKGROUND: There has been no comprehensive study on biochemical characterization of insecticide resistance mechanisms in field populations of Malaysian Culex quinquefasciatus. To fill this void in the literature, a nationwide investigation was performed to quantify the enzyme activities, thereby attempting to characterize the potential resistance mechanisms in Cx. quinquefasciatus in residential areas in Malaysia. METHODOLOGY/PRINCIPAL FINDINGS: Culex quinquefasciatus from 14 residential areas across 13 states and one federal territory were subjected to esterases, mixed function oxidases, glutathione-S-transferase and insensitive acetylcholinesterase assays. Enzyme assays revealed that α-esterases and β-esterases were elevated in 13 populations and 12 populations, respectively. Nine populations demonstrated elevated levels of mixed function oxidases and glutathione-S-transferase. Acetylcholinesterase was insensitive to propoxur in all 14 populations. Activity of α-esterases associated with malathion resistance was found in the present study. In addition, an association between the activity of α-esterases and β-esterases was also demonstrated. CONCLUSIONS/SIGNIFICANCE: The present study has characterized the potential biochemical mechanisms in contributing towards insecticide resistance in Cx. quinquefasciatus field populations in Malaysia. Identification of mechanisms underlying the insecticide resistance will be beneficial in developing effective mosquito control programs in Malaysia. |
format | Online Article Text |
id | pubmed-3836847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38368472013-11-25 Enzymatic Characterization of Insecticide Resistance Mechanisms in Field Populations of Malaysian Culex quinquefasciatus Say (Diptera: Culicidae) Low, Van Lun Chen, Chee Dhang Lee, Han Lim Tan, Tiong Kai Chen, Chin Fong Leong, Cherng Shii Lim, Yvonne Ai Lian Lim, Phaik Eem Norma-Rashid, Yusoff Sofian-Azirun, Mohd PLoS One Research Article BACKGROUND: There has been no comprehensive study on biochemical characterization of insecticide resistance mechanisms in field populations of Malaysian Culex quinquefasciatus. To fill this void in the literature, a nationwide investigation was performed to quantify the enzyme activities, thereby attempting to characterize the potential resistance mechanisms in Cx. quinquefasciatus in residential areas in Malaysia. METHODOLOGY/PRINCIPAL FINDINGS: Culex quinquefasciatus from 14 residential areas across 13 states and one federal territory were subjected to esterases, mixed function oxidases, glutathione-S-transferase and insensitive acetylcholinesterase assays. Enzyme assays revealed that α-esterases and β-esterases were elevated in 13 populations and 12 populations, respectively. Nine populations demonstrated elevated levels of mixed function oxidases and glutathione-S-transferase. Acetylcholinesterase was insensitive to propoxur in all 14 populations. Activity of α-esterases associated with malathion resistance was found in the present study. In addition, an association between the activity of α-esterases and β-esterases was also demonstrated. CONCLUSIONS/SIGNIFICANCE: The present study has characterized the potential biochemical mechanisms in contributing towards insecticide resistance in Cx. quinquefasciatus field populations in Malaysia. Identification of mechanisms underlying the insecticide resistance will be beneficial in developing effective mosquito control programs in Malaysia. Public Library of Science 2013-11-21 /pmc/articles/PMC3836847/ /pubmed/24278220 http://dx.doi.org/10.1371/journal.pone.0079928 Text en © 2013 Low et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Low, Van Lun Chen, Chee Dhang Lee, Han Lim Tan, Tiong Kai Chen, Chin Fong Leong, Cherng Shii Lim, Yvonne Ai Lian Lim, Phaik Eem Norma-Rashid, Yusoff Sofian-Azirun, Mohd Enzymatic Characterization of Insecticide Resistance Mechanisms in Field Populations of Malaysian Culex quinquefasciatus Say (Diptera: Culicidae) |
title | Enzymatic Characterization of Insecticide Resistance Mechanisms in Field Populations of Malaysian Culex quinquefasciatus Say (Diptera: Culicidae) |
title_full | Enzymatic Characterization of Insecticide Resistance Mechanisms in Field Populations of Malaysian Culex quinquefasciatus Say (Diptera: Culicidae) |
title_fullStr | Enzymatic Characterization of Insecticide Resistance Mechanisms in Field Populations of Malaysian Culex quinquefasciatus Say (Diptera: Culicidae) |
title_full_unstemmed | Enzymatic Characterization of Insecticide Resistance Mechanisms in Field Populations of Malaysian Culex quinquefasciatus Say (Diptera: Culicidae) |
title_short | Enzymatic Characterization of Insecticide Resistance Mechanisms in Field Populations of Malaysian Culex quinquefasciatus Say (Diptera: Culicidae) |
title_sort | enzymatic characterization of insecticide resistance mechanisms in field populations of malaysian culex quinquefasciatus say (diptera: culicidae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3836847/ https://www.ncbi.nlm.nih.gov/pubmed/24278220 http://dx.doi.org/10.1371/journal.pone.0079928 |
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