Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection

Polymorphic Amplified Typing Sequences (PATS) is a PCR-based Escherichia coli O157 (O157) strain typing system. Here, we show that PATS compares excellently with Pulsed-Field Gel Electrophoresis (PFGE) in that both methods cluster geographically diverse O157 isolates similarly. Comparative analysis...

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Autores principales: Kudva, Indira T., Smole, Sandra, Griffin, Robert W., Garren, Jeonifer, Kalia, Nimisha, Murray, Megan, John, Manohar, Timperi, Ralph, Calderwood, Stephen B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3837366/
https://www.ncbi.nlm.nih.gov/pubmed/24278089
http://dx.doi.org/10.2174/1874285801307010123
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author Kudva, Indira T.
Smole, Sandra
Griffin, Robert W.
Garren, Jeonifer
Kalia, Nimisha
Murray, Megan
John, Manohar
Timperi, Ralph
Calderwood, Stephen B.
author_facet Kudva, Indira T.
Smole, Sandra
Griffin, Robert W.
Garren, Jeonifer
Kalia, Nimisha
Murray, Megan
John, Manohar
Timperi, Ralph
Calderwood, Stephen B.
author_sort Kudva, Indira T.
collection PubMed
description Polymorphic Amplified Typing Sequences (PATS) is a PCR-based Escherichia coli O157 (O157) strain typing system. Here, we show that PATS compares excellently with Pulsed-Field Gel Electrophoresis (PFGE) in that both methods cluster geographically diverse O157 isolates similarly. Comparative analysis of the results obtained in this simulated “blind” study attests to the discriminating power and applicability of PATS to epidemiological/nosocomial situations.
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spelling pubmed-38373662013-11-25 Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection Kudva, Indira T. Smole, Sandra Griffin, Robert W. Garren, Jeonifer Kalia, Nimisha Murray, Megan John, Manohar Timperi, Ralph Calderwood, Stephen B. Open Microbiol J Article Polymorphic Amplified Typing Sequences (PATS) is a PCR-based Escherichia coli O157 (O157) strain typing system. Here, we show that PATS compares excellently with Pulsed-Field Gel Electrophoresis (PFGE) in that both methods cluster geographically diverse O157 isolates similarly. Comparative analysis of the results obtained in this simulated “blind” study attests to the discriminating power and applicability of PATS to epidemiological/nosocomial situations. Bentham Open 2013-10-31 /pmc/articles/PMC3837366/ /pubmed/24278089 http://dx.doi.org/10.2174/1874285801307010123 Text en © Kudva et al.; Licensee Bentham Open. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Kudva, Indira T.
Smole, Sandra
Griffin, Robert W.
Garren, Jeonifer
Kalia, Nimisha
Murray, Megan
John, Manohar
Timperi, Ralph
Calderwood, Stephen B.
Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection
title Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection
title_full Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection
title_fullStr Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection
title_full_unstemmed Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection
title_short Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection
title_sort polymorphic amplified typing sequences (pats) strain typing system accurately discriminates a set of temporally and spatially disparate escherichia coli o157 isolates associated with human infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3837366/
https://www.ncbi.nlm.nih.gov/pubmed/24278089
http://dx.doi.org/10.2174/1874285801307010123
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