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Complex patterns of mitochondrial dynamics in human pancreatic cells revealed by fluorescent confocal imaging
Mitochondrial morphology and intracellular organization are tightly controlled by the processes of mitochondrial fission–fusion. Moreover, mitochondrial movement and redistribution provide a local ATP supply at cellular sites of particular demands. Here we analysed mitochondrial dynamics in isolated...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3837585/ https://www.ncbi.nlm.nih.gov/pubmed/19382913 http://dx.doi.org/10.1111/j.1582-4934.2009.00750.x |
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author | Kuznetsov, Andrey V Hermann, Martin Troppmair, Jakob Margreiter, Raimund Hengster, Paul |
author_facet | Kuznetsov, Andrey V Hermann, Martin Troppmair, Jakob Margreiter, Raimund Hengster, Paul |
author_sort | Kuznetsov, Andrey V |
collection | PubMed |
description | Mitochondrial morphology and intracellular organization are tightly controlled by the processes of mitochondrial fission–fusion. Moreover, mitochondrial movement and redistribution provide a local ATP supply at cellular sites of particular demands. Here we analysed mitochondrial dynamics in isolated primary human pancreatic cells. Using real time confocal microscopy and mitochondria-specific fluorescent probes tetramethylrhodamine methyl ester and MitoTracker Green we documented complex and novel patterns of spatial and temporal organization of mitochondria, mitochondrial morphology and motility. The most commonly observed types of mitochondrial dynamics were (i) fast fission and fusion; (ii) small oscillating movements of the mitochondrial network; (iii) larger movements, including filament extension, retraction, fast (0.1–0.3 μm/sec.) and frequent oscillating (back and forth) branching in the mitochondrial network; (iv) as well as combinations of these actions and (v) long-distance intracellular translocation of single spherical mitochondria or separated mitochondrial filaments with velocity up to 0.5 μm/sec. Moreover, we show here for the first time, a formation of unusual mitochondrial shapes like rings, loops, and astonishingly even knots created from one or more mitochondrial filaments. These data demonstrate the presence of extensive heterogeneity in mitochondrial morphology and dynamics in living cells under primary culture conditions. In summary, this study reports new patterns of morphological changes and dynamic motion of mitochondria in human pancreatic cells, suggesting an important role of integrations of mitochondria with other intracellular structures and systems. |
format | Online Article Text |
id | pubmed-3837585 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38375852015-04-24 Complex patterns of mitochondrial dynamics in human pancreatic cells revealed by fluorescent confocal imaging Kuznetsov, Andrey V Hermann, Martin Troppmair, Jakob Margreiter, Raimund Hengster, Paul J Cell Mol Med Articles Mitochondrial morphology and intracellular organization are tightly controlled by the processes of mitochondrial fission–fusion. Moreover, mitochondrial movement and redistribution provide a local ATP supply at cellular sites of particular demands. Here we analysed mitochondrial dynamics in isolated primary human pancreatic cells. Using real time confocal microscopy and mitochondria-specific fluorescent probes tetramethylrhodamine methyl ester and MitoTracker Green we documented complex and novel patterns of spatial and temporal organization of mitochondria, mitochondrial morphology and motility. The most commonly observed types of mitochondrial dynamics were (i) fast fission and fusion; (ii) small oscillating movements of the mitochondrial network; (iii) larger movements, including filament extension, retraction, fast (0.1–0.3 μm/sec.) and frequent oscillating (back and forth) branching in the mitochondrial network; (iv) as well as combinations of these actions and (v) long-distance intracellular translocation of single spherical mitochondria or separated mitochondrial filaments with velocity up to 0.5 μm/sec. Moreover, we show here for the first time, a formation of unusual mitochondrial shapes like rings, loops, and astonishingly even knots created from one or more mitochondrial filaments. These data demonstrate the presence of extensive heterogeneity in mitochondrial morphology and dynamics in living cells under primary culture conditions. In summary, this study reports new patterns of morphological changes and dynamic motion of mitochondria in human pancreatic cells, suggesting an important role of integrations of mitochondria with other intracellular structures and systems. Blackwell Publishing Ltd 2010 2009-03-27 /pmc/articles/PMC3837585/ /pubmed/19382913 http://dx.doi.org/10.1111/j.1582-4934.2009.00750.x Text en © 2009 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd |
spellingShingle | Articles Kuznetsov, Andrey V Hermann, Martin Troppmair, Jakob Margreiter, Raimund Hengster, Paul Complex patterns of mitochondrial dynamics in human pancreatic cells revealed by fluorescent confocal imaging |
title | Complex patterns of mitochondrial dynamics in human pancreatic cells revealed by fluorescent confocal imaging |
title_full | Complex patterns of mitochondrial dynamics in human pancreatic cells revealed by fluorescent confocal imaging |
title_fullStr | Complex patterns of mitochondrial dynamics in human pancreatic cells revealed by fluorescent confocal imaging |
title_full_unstemmed | Complex patterns of mitochondrial dynamics in human pancreatic cells revealed by fluorescent confocal imaging |
title_short | Complex patterns of mitochondrial dynamics in human pancreatic cells revealed by fluorescent confocal imaging |
title_sort | complex patterns of mitochondrial dynamics in human pancreatic cells revealed by fluorescent confocal imaging |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3837585/ https://www.ncbi.nlm.nih.gov/pubmed/19382913 http://dx.doi.org/10.1111/j.1582-4934.2009.00750.x |
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