Genetic Marker Suitable for Identification and Genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri

We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimi...

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Detalles Bibliográficos
Autores principales: Tanomsing, Naowarat, Imwong, Mallika, Sutherland, Colin J., Dolecek, Christiane, Hien, Tran Tinh, Nosten, Francois, Day, Nicholas P. J., White, Nicholas J., Snounou, Georges
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2013
Materias:
Parasitology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838052/
https://www.ncbi.nlm.nih.gov/pubmed/24068009
http://dx.doi.org/10.1128/JCM.01527-13
Descripción
Sumario:We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus.

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