Genetic Marker Suitable for Identification and Genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri

We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimi...

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Autores principales: Tanomsing, Naowarat, Imwong, Mallika, Sutherland, Colin J., Dolecek, Christiane, Hien, Tran Tinh, Nosten, Francois, Day, Nicholas P. J., White, Nicholas J., Snounou, Georges
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2013
Materias:
Parasitology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838052/
https://www.ncbi.nlm.nih.gov/pubmed/24068009
http://dx.doi.org/10.1128/JCM.01527-13
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author Tanomsing, Naowarat
Imwong, Mallika
Sutherland, Colin J.
Dolecek, Christiane
Hien, Tran Tinh
Nosten, Francois
Day, Nicholas P. J.
White, Nicholas J.
Snounou, Georges
author_facet Tanomsing, Naowarat
Imwong, Mallika
Sutherland, Colin J.
Dolecek, Christiane
Hien, Tran Tinh
Nosten, Francois
Day, Nicholas P. J.
White, Nicholas J.
Snounou, Georges
author_sort Tanomsing, Naowarat
collection PubMed
description We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus.
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spelling pubmed-38380522013-12-19 Genetic Marker Suitable for Identification and Genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri Tanomsing, Naowarat Imwong, Mallika Sutherland, Colin J. Dolecek, Christiane Hien, Tran Tinh Nosten, Francois Day, Nicholas P. J. White, Nicholas J. Snounou, Georges J Clin Microbiol Parasitology We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus. American Society for Microbiology 2013-12 /pmc/articles/PMC3838052/ /pubmed/24068009 http://dx.doi.org/10.1128/JCM.01527-13 Text en Copyright © 2013 Tanomsing et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Parasitology
Tanomsing, Naowarat
Imwong, Mallika
Sutherland, Colin J.
Dolecek, Christiane
Hien, Tran Tinh
Nosten, Francois
Day, Nicholas P. J.
White, Nicholas J.
Snounou, Georges
Genetic Marker Suitable for Identification and Genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri
title Genetic Marker Suitable for Identification and Genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri
title_full Genetic Marker Suitable for Identification and Genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri
title_fullStr Genetic Marker Suitable for Identification and Genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri
title_full_unstemmed Genetic Marker Suitable for Identification and Genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri
title_short Genetic Marker Suitable for Identification and Genotyping of Plasmodium ovale curtisi and Plasmodium ovale wallikeri
title_sort genetic marker suitable for identification and genotyping of plasmodium ovale curtisi and plasmodium ovale wallikeri
topic Parasitology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838052/
https://www.ncbi.nlm.nih.gov/pubmed/24068009
http://dx.doi.org/10.1128/JCM.01527-13
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