A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput

Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a m...

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Detalles Bibliográficos
Autores principales: Siridechadilok, Bunpote, Gomutsukhavadee, Methee, Sawaengpol, Thunyarat, Sangiambut, Sutha, Puttikhunt, Chunya, Chin-inmanu, Kwanrutai, Suriyaphol, Prapat, Malasit, Prida, Screaton, Gavin, Mongkolsapaya, Juthathip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2013
Materias:
Genome Replication and Regulation of Viral Gene Expression
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838137/
https://www.ncbi.nlm.nih.gov/pubmed/24049164
http://dx.doi.org/10.1128/JVI.02261-13
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author Siridechadilok, Bunpote
Gomutsukhavadee, Methee
Sawaengpol, Thunyarat
Sangiambut, Sutha
Puttikhunt, Chunya
Chin-inmanu, Kwanrutai
Suriyaphol, Prapat
Malasit, Prida
Screaton, Gavin
Mongkolsapaya, Juthathip
author_facet Siridechadilok, Bunpote
Gomutsukhavadee, Methee
Sawaengpol, Thunyarat
Sangiambut, Sutha
Puttikhunt, Chunya
Chin-inmanu, Kwanrutai
Suriyaphol, Prapat
Malasit, Prida
Screaton, Gavin
Mongkolsapaya, Juthathip
author_sort Siridechadilok, Bunpote
collection PubMed
description Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses.
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spelling pubmed-38381372013-12-18 A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput Siridechadilok, Bunpote Gomutsukhavadee, Methee Sawaengpol, Thunyarat Sangiambut, Sutha Puttikhunt, Chunya Chin-inmanu, Kwanrutai Suriyaphol, Prapat Malasit, Prida Screaton, Gavin Mongkolsapaya, Juthathip J Virol Genome Replication and Regulation of Viral Gene Expression Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses. American Society for Microbiology 2013-12 /pmc/articles/PMC3838137/ /pubmed/24049164 http://dx.doi.org/10.1128/JVI.02261-13 Text en Copyright © 2013 Siridechadilok et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Genome Replication and Regulation of Viral Gene Expression
Siridechadilok, Bunpote
Gomutsukhavadee, Methee
Sawaengpol, Thunyarat
Sangiambut, Sutha
Puttikhunt, Chunya
Chin-inmanu, Kwanrutai
Suriyaphol, Prapat
Malasit, Prida
Screaton, Gavin
Mongkolsapaya, Juthathip
A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput
title A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput
title_full A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput
title_fullStr A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput
title_full_unstemmed A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput
title_short A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput
title_sort simplified positive-sense-rna virus construction approach that enhances analysis throughput
topic Genome Replication and Regulation of Viral Gene Expression
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838137/
https://www.ncbi.nlm.nih.gov/pubmed/24049164
http://dx.doi.org/10.1128/JVI.02261-13
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