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A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput
Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a m...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838137/ https://www.ncbi.nlm.nih.gov/pubmed/24049164 http://dx.doi.org/10.1128/JVI.02261-13 |