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Detection of Specific IgA Antibodies against a Novel Deamidated 8-Mer Gliadin Peptide in Blood Plasma Samples from Celiac Patients

We studied whether celiac disease (CD) patients produce antibodies against a novel gliadin peptide specifically generated in the duodenum of CD patients by a previously described pattern of CD-specific duodenal proteases. Fingerprinting and ion-trap mass spectrometry of CD-specific duodenal gliadin-...

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Autores principales: Vallejo-Diez, Sara, Bernardo, David, Moreno, María de Lourdes, Muñoz-Suano, Alba, Fernández-Salazar, Luis, Calvo, Carmen, Sousa, Carolina, Garrote, José A., Cebolla, Ángel, Arranz, Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838339/
https://www.ncbi.nlm.nih.gov/pubmed/24278359
http://dx.doi.org/10.1371/journal.pone.0080982
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author Vallejo-Diez, Sara
Bernardo, David
Moreno, María de Lourdes
Muñoz-Suano, Alba
Fernández-Salazar, Luis
Calvo, Carmen
Sousa, Carolina
Garrote, José A.
Cebolla, Ángel
Arranz, Eduardo
author_facet Vallejo-Diez, Sara
Bernardo, David
Moreno, María de Lourdes
Muñoz-Suano, Alba
Fernández-Salazar, Luis
Calvo, Carmen
Sousa, Carolina
Garrote, José A.
Cebolla, Ángel
Arranz, Eduardo
author_sort Vallejo-Diez, Sara
collection PubMed
description We studied whether celiac disease (CD) patients produce antibodies against a novel gliadin peptide specifically generated in the duodenum of CD patients by a previously described pattern of CD-specific duodenal proteases. Fingerprinting and ion-trap mass spectrometry of CD-specific duodenal gliadin-degrading protease pattern revealed a new 8-mer gliadin-derived peptide. An ELISA against synthetic deamidated 8-mer peptides (DGP 8-mer) was used to study the presence of IgA anti-DGP 8-mer antibodies in plasma samples from 81 children (31 active CD patients (aCD), 17 CD patients on a gluten-free diet (GFD), 10 healthy controls (C) and 23 patients with other gastrointestinal pathology (GP)) and 101 adults (16 aCD, 12 GFD, 27 C and 46 GP-patients). Deamidation of the 8-mer peptide significantly increased the reactivity of the IgA antibodies from CD patients against the peptide. Significant IgA anti-DGP 8-mer antibodies levels were detected in 93.5% of aCD-, 11.8% of GFD- and 4.3% of GP-patients in children. In adults, antibodies were detected in 81.3% of aCD-patients and 8.3% of GFD-patients while were absent in 100% of C- and GP-patients. Duodenal CD-specific gliadin degrading proteases release an 8-mer gliadin peptide that once deamidated is an antigen for specific IgA antibodies in CD patients which may provide a new accurate diagnostic tool in CD.
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spelling pubmed-38383392013-11-25 Detection of Specific IgA Antibodies against a Novel Deamidated 8-Mer Gliadin Peptide in Blood Plasma Samples from Celiac Patients Vallejo-Diez, Sara Bernardo, David Moreno, María de Lourdes Muñoz-Suano, Alba Fernández-Salazar, Luis Calvo, Carmen Sousa, Carolina Garrote, José A. Cebolla, Ángel Arranz, Eduardo PLoS One Research Article We studied whether celiac disease (CD) patients produce antibodies against a novel gliadin peptide specifically generated in the duodenum of CD patients by a previously described pattern of CD-specific duodenal proteases. Fingerprinting and ion-trap mass spectrometry of CD-specific duodenal gliadin-degrading protease pattern revealed a new 8-mer gliadin-derived peptide. An ELISA against synthetic deamidated 8-mer peptides (DGP 8-mer) was used to study the presence of IgA anti-DGP 8-mer antibodies in plasma samples from 81 children (31 active CD patients (aCD), 17 CD patients on a gluten-free diet (GFD), 10 healthy controls (C) and 23 patients with other gastrointestinal pathology (GP)) and 101 adults (16 aCD, 12 GFD, 27 C and 46 GP-patients). Deamidation of the 8-mer peptide significantly increased the reactivity of the IgA antibodies from CD patients against the peptide. Significant IgA anti-DGP 8-mer antibodies levels were detected in 93.5% of aCD-, 11.8% of GFD- and 4.3% of GP-patients in children. In adults, antibodies were detected in 81.3% of aCD-patients and 8.3% of GFD-patients while were absent in 100% of C- and GP-patients. Duodenal CD-specific gliadin degrading proteases release an 8-mer gliadin peptide that once deamidated is an antigen for specific IgA antibodies in CD patients which may provide a new accurate diagnostic tool in CD. Public Library of Science 2013-11-22 /pmc/articles/PMC3838339/ /pubmed/24278359 http://dx.doi.org/10.1371/journal.pone.0080982 Text en © 2013 Vallejo-Diez et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Vallejo-Diez, Sara
Bernardo, David
Moreno, María de Lourdes
Muñoz-Suano, Alba
Fernández-Salazar, Luis
Calvo, Carmen
Sousa, Carolina
Garrote, José A.
Cebolla, Ángel
Arranz, Eduardo
Detection of Specific IgA Antibodies against a Novel Deamidated 8-Mer Gliadin Peptide in Blood Plasma Samples from Celiac Patients
title Detection of Specific IgA Antibodies against a Novel Deamidated 8-Mer Gliadin Peptide in Blood Plasma Samples from Celiac Patients
title_full Detection of Specific IgA Antibodies against a Novel Deamidated 8-Mer Gliadin Peptide in Blood Plasma Samples from Celiac Patients
title_fullStr Detection of Specific IgA Antibodies against a Novel Deamidated 8-Mer Gliadin Peptide in Blood Plasma Samples from Celiac Patients
title_full_unstemmed Detection of Specific IgA Antibodies against a Novel Deamidated 8-Mer Gliadin Peptide in Blood Plasma Samples from Celiac Patients
title_short Detection of Specific IgA Antibodies against a Novel Deamidated 8-Mer Gliadin Peptide in Blood Plasma Samples from Celiac Patients
title_sort detection of specific iga antibodies against a novel deamidated 8-mer gliadin peptide in blood plasma samples from celiac patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838339/
https://www.ncbi.nlm.nih.gov/pubmed/24278359
http://dx.doi.org/10.1371/journal.pone.0080982
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