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Hypotensive and Vasorelaxant Effects of Sericin-Derived Oligopeptides in Rats

Sericin-derived oligopeptides obtained from silk cocoons were investigated for the in vivo hypotensive effect and investigated for the underlying mechanism involved in vasodilation in isolated rat thoracic aorta. In normotensive anesthetized rats, oligopeptides induced an immediate and transient hyp...

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Autores principales: Onsa-ard, Amnart, Shimbhu, Dawan, Tocharus, Jiraporn, Sutheerawattananonda, Manote, Pantan, Rungusa, Tocharus, Chainarong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839117/
https://www.ncbi.nlm.nih.gov/pubmed/24312733
http://dx.doi.org/10.1155/2013/717529
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author Onsa-ard, Amnart
Shimbhu, Dawan
Tocharus, Jiraporn
Sutheerawattananonda, Manote
Pantan, Rungusa
Tocharus, Chainarong
author_facet Onsa-ard, Amnart
Shimbhu, Dawan
Tocharus, Jiraporn
Sutheerawattananonda, Manote
Pantan, Rungusa
Tocharus, Chainarong
author_sort Onsa-ard, Amnart
collection PubMed
description Sericin-derived oligopeptides obtained from silk cocoons were investigated for the in vivo hypotensive effect and investigated for the underlying mechanism involved in vasodilation in isolated rat thoracic aorta. In normotensive anesthetized rats, oligopeptides induced an immediate and transient hypotensive activity. In rat aortic rings, oligopeptides induced a concentration-dependent vasorelaxation in vessels precontracted with both KCl and phenylephrine (PE) with endothelium-intact or endothelium-denuded rings. In endothelium-intact rings, pretreatment with Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME, 100 µM), an inhibitor of the NO synthase (NOS) or 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, 1 µM), a selective inhibitor of the guanylyl cyclase enzyme, significantly reduced the relaxant effect of oligopeptides. However, indomethacin, an inhibitor of the cyclooxygenase, had no effect on oligopeptides-induced relaxation. In addition, pretreatment with tetraethylammonium (TEA, 5 mM) reduced the maximal relaxant effect induced by oligopeptides. By contrast, relaxation was not affected by 4-aminopyridine (4-AP, 1 mM), glibenclamide (10 µM), or barium chloride (BaCl(2), 1 mM). In depolarization Ca(2+)-free solution, oligopeptides inhibited calcium chloride- (CaCl(2)-) induced contraction in endothelium-denuded rings in a concentration-dependent manner. Nevertheless, oligopeptides attenuated transient contractions in Ca(2+)-free medium containing EGTA (1 mM) induced by 1 µM PE, but they were not affected by 20 mM caffeine. It is obvious that potent vasodilation effect of oligopeptides is mediated through both the endothelium and the vascular smooth muscle.
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spelling pubmed-38391172013-12-05 Hypotensive and Vasorelaxant Effects of Sericin-Derived Oligopeptides in Rats Onsa-ard, Amnart Shimbhu, Dawan Tocharus, Jiraporn Sutheerawattananonda, Manote Pantan, Rungusa Tocharus, Chainarong ISRN Pharmacol Research Article Sericin-derived oligopeptides obtained from silk cocoons were investigated for the in vivo hypotensive effect and investigated for the underlying mechanism involved in vasodilation in isolated rat thoracic aorta. In normotensive anesthetized rats, oligopeptides induced an immediate and transient hypotensive activity. In rat aortic rings, oligopeptides induced a concentration-dependent vasorelaxation in vessels precontracted with both KCl and phenylephrine (PE) with endothelium-intact or endothelium-denuded rings. In endothelium-intact rings, pretreatment with Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME, 100 µM), an inhibitor of the NO synthase (NOS) or 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, 1 µM), a selective inhibitor of the guanylyl cyclase enzyme, significantly reduced the relaxant effect of oligopeptides. However, indomethacin, an inhibitor of the cyclooxygenase, had no effect on oligopeptides-induced relaxation. In addition, pretreatment with tetraethylammonium (TEA, 5 mM) reduced the maximal relaxant effect induced by oligopeptides. By contrast, relaxation was not affected by 4-aminopyridine (4-AP, 1 mM), glibenclamide (10 µM), or barium chloride (BaCl(2), 1 mM). In depolarization Ca(2+)-free solution, oligopeptides inhibited calcium chloride- (CaCl(2)-) induced contraction in endothelium-denuded rings in a concentration-dependent manner. Nevertheless, oligopeptides attenuated transient contractions in Ca(2+)-free medium containing EGTA (1 mM) induced by 1 µM PE, but they were not affected by 20 mM caffeine. It is obvious that potent vasodilation effect of oligopeptides is mediated through both the endothelium and the vascular smooth muscle. Hindawi Publishing Corporation 2013-11-07 /pmc/articles/PMC3839117/ /pubmed/24312733 http://dx.doi.org/10.1155/2013/717529 Text en Copyright © 2013 Amnart Onsa-ard et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Onsa-ard, Amnart
Shimbhu, Dawan
Tocharus, Jiraporn
Sutheerawattananonda, Manote
Pantan, Rungusa
Tocharus, Chainarong
Hypotensive and Vasorelaxant Effects of Sericin-Derived Oligopeptides in Rats
title Hypotensive and Vasorelaxant Effects of Sericin-Derived Oligopeptides in Rats
title_full Hypotensive and Vasorelaxant Effects of Sericin-Derived Oligopeptides in Rats
title_fullStr Hypotensive and Vasorelaxant Effects of Sericin-Derived Oligopeptides in Rats
title_full_unstemmed Hypotensive and Vasorelaxant Effects of Sericin-Derived Oligopeptides in Rats
title_short Hypotensive and Vasorelaxant Effects of Sericin-Derived Oligopeptides in Rats
title_sort hypotensive and vasorelaxant effects of sericin-derived oligopeptides in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839117/
https://www.ncbi.nlm.nih.gov/pubmed/24312733
http://dx.doi.org/10.1155/2013/717529
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