Cargando…
Super-resolution microscopy in studying neuroendocrine cell function
The last two decades have seen a tremendous development in high resolution microscopy techniques giving rise to acronyms such as TIRFM, SIM, PALM, STORM, and STED. The goal of all these techniques is to overcome the physical resolution barrier of light microscopy in order to resolve precise protein...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839409/ https://www.ncbi.nlm.nih.gov/pubmed/24324394 http://dx.doi.org/10.3389/fnins.2013.00222 |
_version_ | 1782478428167995392 |
---|---|
author | Bost, Anneka Pasche, Mathias Schirra, Claudia Becherer, Ute |
author_facet | Bost, Anneka Pasche, Mathias Schirra, Claudia Becherer, Ute |
author_sort | Bost, Anneka |
collection | PubMed |
description | The last two decades have seen a tremendous development in high resolution microscopy techniques giving rise to acronyms such as TIRFM, SIM, PALM, STORM, and STED. The goal of all these techniques is to overcome the physical resolution barrier of light microscopy in order to resolve precise protein localization and possibly their interaction in cells. Neuroendocrine cell function is to secrete hormones and peptides on demand. This fine-tuned multi-step process is mediated by a large array of proteins. Here, we review the new microscopy techniques used to obtain high resolution and how they have been applied to increase our knowledge of the molecular mechanisms involved in neuroendocrine cell secretion. Further the limitations of these methods are discussed and insights in possible new applications are provided. |
format | Online Article Text |
id | pubmed-3839409 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-38394092013-12-09 Super-resolution microscopy in studying neuroendocrine cell function Bost, Anneka Pasche, Mathias Schirra, Claudia Becherer, Ute Front Neurosci Endocrinology The last two decades have seen a tremendous development in high resolution microscopy techniques giving rise to acronyms such as TIRFM, SIM, PALM, STORM, and STED. The goal of all these techniques is to overcome the physical resolution barrier of light microscopy in order to resolve precise protein localization and possibly their interaction in cells. Neuroendocrine cell function is to secrete hormones and peptides on demand. This fine-tuned multi-step process is mediated by a large array of proteins. Here, we review the new microscopy techniques used to obtain high resolution and how they have been applied to increase our knowledge of the molecular mechanisms involved in neuroendocrine cell secretion. Further the limitations of these methods are discussed and insights in possible new applications are provided. Frontiers Media S.A. 2013-11-25 /pmc/articles/PMC3839409/ /pubmed/24324394 http://dx.doi.org/10.3389/fnins.2013.00222 Text en Copyright © 2013 Bost, Pasche, Schirra and Becherer. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Bost, Anneka Pasche, Mathias Schirra, Claudia Becherer, Ute Super-resolution microscopy in studying neuroendocrine cell function |
title | Super-resolution microscopy in studying neuroendocrine cell function |
title_full | Super-resolution microscopy in studying neuroendocrine cell function |
title_fullStr | Super-resolution microscopy in studying neuroendocrine cell function |
title_full_unstemmed | Super-resolution microscopy in studying neuroendocrine cell function |
title_short | Super-resolution microscopy in studying neuroendocrine cell function |
title_sort | super-resolution microscopy in studying neuroendocrine cell function |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839409/ https://www.ncbi.nlm.nih.gov/pubmed/24324394 http://dx.doi.org/10.3389/fnins.2013.00222 |
work_keys_str_mv | AT bostanneka superresolutionmicroscopyinstudyingneuroendocrinecellfunction AT paschemathias superresolutionmicroscopyinstudyingneuroendocrinecellfunction AT schirraclaudia superresolutionmicroscopyinstudyingneuroendocrinecellfunction AT bechererute superresolutionmicroscopyinstudyingneuroendocrinecellfunction |