Cargando…

Role of the hypoxia response pathway in lens formation during embryonic development of Xenopus laevis()

The RING finger ubiquitin ligase seven in absentia homolog 2 (Siah2) was identified in the R7 photoreceptor cells of Drosophila melanogaster, and it regulates the stability of prolyl hydroxylase domains (PHDs), with a concomitant effect on HIF-1α availability in the hypoxia response pathway. We prev...

Descripción completa

Detalles Bibliográficos
Autores principales: Baba, Kazunobu, Muraguchi, Taichi, Imaoka, Susumu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839852/
https://www.ncbi.nlm.nih.gov/pubmed/24282676
http://dx.doi.org/10.1016/j.fob.2013.10.006
_version_ 1782478443788632064
author Baba, Kazunobu
Muraguchi, Taichi
Imaoka, Susumu
author_facet Baba, Kazunobu
Muraguchi, Taichi
Imaoka, Susumu
author_sort Baba, Kazunobu
collection PubMed
description The RING finger ubiquitin ligase seven in absentia homolog 2 (Siah2) was identified in the R7 photoreceptor cells of Drosophila melanogaster, and it regulates the stability of prolyl hydroxylase domains (PHDs), with a concomitant effect on HIF-1α availability in the hypoxia response pathway. We previously reported that the hypoxia response pathway contributes to eye development during the embryonic development of Xenopus laevis. In this paper, the role of Siah2-mediated hypoxia response pathway in eye development of X. laevis embryos was further characterized. Xenopus Siah2 (xSiah2) mRNA was detected in lens tissue and xSiah2 overexpression caused a thickened lens placode, leading to loss of the optic lens. In embryos overexpressing xSiah2, lens marker gene transcription was reduced, suggesting that xSiah2 contributes to lens formation. xSiah2 overexpression decreased Xenopus PHD accumulation and increased Xenopus HIF-1α (xHIF-1α) accumulation. xHIF-1α degeneration with resveratrol restored the optical abnormality caused by xSiah2 overexpression, suggesting that the xSiah2-mediated hypoxia response pathway contributes to lens formation. Moreover, xSiah2 overexpression decreased endothelial–mesenchymal transition (EMT)-related Notch signaling-responsive genes transcription during the invasion of the lens placode. Our results suggest that the hypoxia response pathway plays an important role in the regulation of the EMT via the Notch signaling pathway during lens formation.
format Online
Article
Text
id pubmed-3839852
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-38398522013-11-26 Role of the hypoxia response pathway in lens formation during embryonic development of Xenopus laevis() Baba, Kazunobu Muraguchi, Taichi Imaoka, Susumu FEBS Open Bio Article The RING finger ubiquitin ligase seven in absentia homolog 2 (Siah2) was identified in the R7 photoreceptor cells of Drosophila melanogaster, and it regulates the stability of prolyl hydroxylase domains (PHDs), with a concomitant effect on HIF-1α availability in the hypoxia response pathway. We previously reported that the hypoxia response pathway contributes to eye development during the embryonic development of Xenopus laevis. In this paper, the role of Siah2-mediated hypoxia response pathway in eye development of X. laevis embryos was further characterized. Xenopus Siah2 (xSiah2) mRNA was detected in lens tissue and xSiah2 overexpression caused a thickened lens placode, leading to loss of the optic lens. In embryos overexpressing xSiah2, lens marker gene transcription was reduced, suggesting that xSiah2 contributes to lens formation. xSiah2 overexpression decreased Xenopus PHD accumulation and increased Xenopus HIF-1α (xHIF-1α) accumulation. xHIF-1α degeneration with resveratrol restored the optical abnormality caused by xSiah2 overexpression, suggesting that the xSiah2-mediated hypoxia response pathway contributes to lens formation. Moreover, xSiah2 overexpression decreased endothelial–mesenchymal transition (EMT)-related Notch signaling-responsive genes transcription during the invasion of the lens placode. Our results suggest that the hypoxia response pathway plays an important role in the regulation of the EMT via the Notch signaling pathway during lens formation. Elsevier 2013-10-23 /pmc/articles/PMC3839852/ /pubmed/24282676 http://dx.doi.org/10.1016/j.fob.2013.10.006 Text en © 2013 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Baba, Kazunobu
Muraguchi, Taichi
Imaoka, Susumu
Role of the hypoxia response pathway in lens formation during embryonic development of Xenopus laevis()
title Role of the hypoxia response pathway in lens formation during embryonic development of Xenopus laevis()
title_full Role of the hypoxia response pathway in lens formation during embryonic development of Xenopus laevis()
title_fullStr Role of the hypoxia response pathway in lens formation during embryonic development of Xenopus laevis()
title_full_unstemmed Role of the hypoxia response pathway in lens formation during embryonic development of Xenopus laevis()
title_short Role of the hypoxia response pathway in lens formation during embryonic development of Xenopus laevis()
title_sort role of the hypoxia response pathway in lens formation during embryonic development of xenopus laevis()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839852/
https://www.ncbi.nlm.nih.gov/pubmed/24282676
http://dx.doi.org/10.1016/j.fob.2013.10.006
work_keys_str_mv AT babakazunobu roleofthehypoxiaresponsepathwayinlensformationduringembryonicdevelopmentofxenopuslaevis
AT muraguchitaichi roleofthehypoxiaresponsepathwayinlensformationduringembryonicdevelopmentofxenopuslaevis
AT imaokasusumu roleofthehypoxiaresponsepathwayinlensformationduringembryonicdevelopmentofxenopuslaevis