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Lulu Regulates Shroom-Induced Apical Constriction during Neural Tube Closure

Apical constriction is an essential cell behavior during neural tube closure, but its underlying mechanisms are not fully understood. Lulu, or EPB4.1l5, is a FERM domain protein that has been implicated in apical constriction and actomyosin contractility in mouse embryos and cultured cells. Interfer...

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Autores principales: Chu, Chih-Wen, Gerstenzang, Emma, Ossipova, Olga, Sokol, Sergei Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839891/
https://www.ncbi.nlm.nih.gov/pubmed/24282618
http://dx.doi.org/10.1371/journal.pone.0081854
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author Chu, Chih-Wen
Gerstenzang, Emma
Ossipova, Olga
Sokol, Sergei Y.
author_facet Chu, Chih-Wen
Gerstenzang, Emma
Ossipova, Olga
Sokol, Sergei Y.
author_sort Chu, Chih-Wen
collection PubMed
description Apical constriction is an essential cell behavior during neural tube closure, but its underlying mechanisms are not fully understood. Lulu, or EPB4.1l5, is a FERM domain protein that has been implicated in apical constriction and actomyosin contractility in mouse embryos and cultured cells. Interference with the function of Lulu in Xenopus embryos by a specific antisense morpholino oligonucleotide or a carboxy-terminal fragment of Lulu impaired apical constriction during neural plate hinge formation. This effect was likely due to lack of actomyosin contractility in superficial neuroectodermal cells. By contrast, overexpression of Lulu RNA in embryonic ectoderm cells triggered ectopic apico-basal elongation and apical constriction, accompanied by the apical recruitment of F-actin. Depletion of endogenous Lulu disrupted the localization and activity of Shroom3, a PDZ-containing actin-binding protein that has also been implicated in apical constriction. Furthermore, Lulu and Shroom3 RNAs cooperated in triggering ectopic apical constriction in embryonic ectoderm. Our findings reveal that Lulu is essential for Shroom3-dependent apical constriction during vertebrate neural tube closure.
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spelling pubmed-38398912013-11-26 Lulu Regulates Shroom-Induced Apical Constriction during Neural Tube Closure Chu, Chih-Wen Gerstenzang, Emma Ossipova, Olga Sokol, Sergei Y. PLoS One Research Article Apical constriction is an essential cell behavior during neural tube closure, but its underlying mechanisms are not fully understood. Lulu, or EPB4.1l5, is a FERM domain protein that has been implicated in apical constriction and actomyosin contractility in mouse embryos and cultured cells. Interference with the function of Lulu in Xenopus embryos by a specific antisense morpholino oligonucleotide or a carboxy-terminal fragment of Lulu impaired apical constriction during neural plate hinge formation. This effect was likely due to lack of actomyosin contractility in superficial neuroectodermal cells. By contrast, overexpression of Lulu RNA in embryonic ectoderm cells triggered ectopic apico-basal elongation and apical constriction, accompanied by the apical recruitment of F-actin. Depletion of endogenous Lulu disrupted the localization and activity of Shroom3, a PDZ-containing actin-binding protein that has also been implicated in apical constriction. Furthermore, Lulu and Shroom3 RNAs cooperated in triggering ectopic apical constriction in embryonic ectoderm. Our findings reveal that Lulu is essential for Shroom3-dependent apical constriction during vertebrate neural tube closure. Public Library of Science 2013-11-25 /pmc/articles/PMC3839891/ /pubmed/24282618 http://dx.doi.org/10.1371/journal.pone.0081854 Text en © 2013 Chu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chu, Chih-Wen
Gerstenzang, Emma
Ossipova, Olga
Sokol, Sergei Y.
Lulu Regulates Shroom-Induced Apical Constriction during Neural Tube Closure
title Lulu Regulates Shroom-Induced Apical Constriction during Neural Tube Closure
title_full Lulu Regulates Shroom-Induced Apical Constriction during Neural Tube Closure
title_fullStr Lulu Regulates Shroom-Induced Apical Constriction during Neural Tube Closure
title_full_unstemmed Lulu Regulates Shroom-Induced Apical Constriction during Neural Tube Closure
title_short Lulu Regulates Shroom-Induced Apical Constriction during Neural Tube Closure
title_sort lulu regulates shroom-induced apical constriction during neural tube closure
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839891/
https://www.ncbi.nlm.nih.gov/pubmed/24282618
http://dx.doi.org/10.1371/journal.pone.0081854
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