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The cytotoxic effect of α-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction

In recent years, α-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of α-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibiti...

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Autores principales: SUCHA, LENKA, HROCH, MILOS, REZACOVA, MARTINA, RUDOLF, EMIL, HAVELEK, RADIM, SISPERA, LUDEK, CMIELOVA, JANA, KOHLEROVA, RENATA, BEZROUK, ALES, TOMSIK, PAVEL
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839989/
https://www.ncbi.nlm.nih.gov/pubmed/24100733
http://dx.doi.org/10.3892/or.2013.2778
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author SUCHA, LENKA
HROCH, MILOS
REZACOVA, MARTINA
RUDOLF, EMIL
HAVELEK, RADIM
SISPERA, LUDEK
CMIELOVA, JANA
KOHLEROVA, RENATA
BEZROUK, ALES
TOMSIK, PAVEL
author_facet SUCHA, LENKA
HROCH, MILOS
REZACOVA, MARTINA
RUDOLF, EMIL
HAVELEK, RADIM
SISPERA, LUDEK
CMIELOVA, JANA
KOHLEROVA, RENATA
BEZROUK, ALES
TOMSIK, PAVEL
author_sort SUCHA, LENKA
collection PubMed
description In recent years, α-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of α-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 μM but then a recovery of cells occurred. The recovery was not caused by the biotransformation of α-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of α-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of α-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in α-tomatine-treated cells. These results support the conclusion that α-tomatine does not induce apoptosis in the MCF-7 cell line.
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spelling pubmed-38399892013-11-26 The cytotoxic effect of α-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction SUCHA, LENKA HROCH, MILOS REZACOVA, MARTINA RUDOLF, EMIL HAVELEK, RADIM SISPERA, LUDEK CMIELOVA, JANA KOHLEROVA, RENATA BEZROUK, ALES TOMSIK, PAVEL Oncol Rep Articles In recent years, α-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of α-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 μM but then a recovery of cells occurred. The recovery was not caused by the biotransformation of α-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of α-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of α-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in α-tomatine-treated cells. These results support the conclusion that α-tomatine does not induce apoptosis in the MCF-7 cell line. D.A. Spandidos 2013-12 2013-10-02 /pmc/articles/PMC3839989/ /pubmed/24100733 http://dx.doi.org/10.3892/or.2013.2778 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
SUCHA, LENKA
HROCH, MILOS
REZACOVA, MARTINA
RUDOLF, EMIL
HAVELEK, RADIM
SISPERA, LUDEK
CMIELOVA, JANA
KOHLEROVA, RENATA
BEZROUK, ALES
TOMSIK, PAVEL
The cytotoxic effect of α-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction
title The cytotoxic effect of α-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction
title_full The cytotoxic effect of α-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction
title_fullStr The cytotoxic effect of α-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction
title_full_unstemmed The cytotoxic effect of α-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction
title_short The cytotoxic effect of α-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction
title_sort cytotoxic effect of α-tomatine in mcf-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839989/
https://www.ncbi.nlm.nih.gov/pubmed/24100733
http://dx.doi.org/10.3892/or.2013.2778
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