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Functional characterization of genetic enzyme variations in human lipoxygenases()

Mammalian lipoxygenases play a role in normal cell development and differentiation but they have also been implicated in the pathogenesis of cardiovascular, hyperproliferative and neurodegenerative diseases. As lipid peroxidizing enzymes they are involved in the regulation of cellular redox homeosta...

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Autores principales: Horn, Thomas, Reddy Kakularam, Kumar, Anton, Monika, Richter, Constanze, Reddanna, Pallu, Kuhn, Hartmut
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840004/
https://www.ncbi.nlm.nih.gov/pubmed/24282679
http://dx.doi.org/10.1016/j.redox.2013.11.001
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author Horn, Thomas
Reddy Kakularam, Kumar
Anton, Monika
Richter, Constanze
Reddanna, Pallu
Kuhn, Hartmut
author_facet Horn, Thomas
Reddy Kakularam, Kumar
Anton, Monika
Richter, Constanze
Reddanna, Pallu
Kuhn, Hartmut
author_sort Horn, Thomas
collection PubMed
description Mammalian lipoxygenases play a role in normal cell development and differentiation but they have also been implicated in the pathogenesis of cardiovascular, hyperproliferative and neurodegenerative diseases. As lipid peroxidizing enzymes they are involved in the regulation of cellular redox homeostasis since they produce lipid hydroperoxides, which serve as an efficient source for free radicals. There are various epidemiological correlation studies relating naturally occurring variations in the six human lipoxygenase genes (SNPs or rare mutations) to the frequency for various diseases in these individuals, but for most of the described variations no functional data are available. Employing a combined bioinformatical and enzymological strategy, which included structural modeling and experimental site-directed mutagenesis, we systematically explored the structural and functional consequences of non-synonymous genetic variations in four different human lipoxygenase genes (ALOX5, ALOX12, ALOX15, and ALOX15B) that have been identified in the human 1000 genome project. Due to a lack of a functional expression system we resigned to analyze the functionality of genetic variations in the hALOX12B and hALOXE3 gene. We found that most of the frequent non-synonymous coding SNPs are located at the enzyme surface and hardly alter the enzyme functionality. In contrast, genetic variations which affect functional important amino acid residues or lead to truncated enzyme variations (nonsense mutations) are usually rare with a global allele frequency<0.1%. This data suggest that there appears to be an evolutionary pressure on the coding regions of the lipoxygenase genes preventing the accumulation of loss-of-function variations in the human population.
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spelling pubmed-38400042013-11-26 Functional characterization of genetic enzyme variations in human lipoxygenases() Horn, Thomas Reddy Kakularam, Kumar Anton, Monika Richter, Constanze Reddanna, Pallu Kuhn, Hartmut Redox Biol Article Mammalian lipoxygenases play a role in normal cell development and differentiation but they have also been implicated in the pathogenesis of cardiovascular, hyperproliferative and neurodegenerative diseases. As lipid peroxidizing enzymes they are involved in the regulation of cellular redox homeostasis since they produce lipid hydroperoxides, which serve as an efficient source for free radicals. There are various epidemiological correlation studies relating naturally occurring variations in the six human lipoxygenase genes (SNPs or rare mutations) to the frequency for various diseases in these individuals, but for most of the described variations no functional data are available. Employing a combined bioinformatical and enzymological strategy, which included structural modeling and experimental site-directed mutagenesis, we systematically explored the structural and functional consequences of non-synonymous genetic variations in four different human lipoxygenase genes (ALOX5, ALOX12, ALOX15, and ALOX15B) that have been identified in the human 1000 genome project. Due to a lack of a functional expression system we resigned to analyze the functionality of genetic variations in the hALOX12B and hALOXE3 gene. We found that most of the frequent non-synonymous coding SNPs are located at the enzyme surface and hardly alter the enzyme functionality. In contrast, genetic variations which affect functional important amino acid residues or lead to truncated enzyme variations (nonsense mutations) are usually rare with a global allele frequency<0.1%. This data suggest that there appears to be an evolutionary pressure on the coding regions of the lipoxygenase genes preventing the accumulation of loss-of-function variations in the human population. Elsevier 2013-11-11 /pmc/articles/PMC3840004/ /pubmed/24282679 http://dx.doi.org/10.1016/j.redox.2013.11.001 Text en © 2013 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Horn, Thomas
Reddy Kakularam, Kumar
Anton, Monika
Richter, Constanze
Reddanna, Pallu
Kuhn, Hartmut
Functional characterization of genetic enzyme variations in human lipoxygenases()
title Functional characterization of genetic enzyme variations in human lipoxygenases()
title_full Functional characterization of genetic enzyme variations in human lipoxygenases()
title_fullStr Functional characterization of genetic enzyme variations in human lipoxygenases()
title_full_unstemmed Functional characterization of genetic enzyme variations in human lipoxygenases()
title_short Functional characterization of genetic enzyme variations in human lipoxygenases()
title_sort functional characterization of genetic enzyme variations in human lipoxygenases()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840004/
https://www.ncbi.nlm.nih.gov/pubmed/24282679
http://dx.doi.org/10.1016/j.redox.2013.11.001
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