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Molecular Insights of Genetic Variation in Erianthus arundinaceus Populations Native to China

BACKGROUND: E. arundinaceus (Retz.) Jeswiet is a warm-season, tall-growing perennial species native to much southern portion in China. The grass has been extensively used in sugarcane breeding and is recently targeted as a bioenergy feedstock crop. However, information on the genetic structure of th...

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Autores principales: Zhang, Jianbo, Yan, Jiajun, Zhang, Yunwei, Ma, Xiao, Bai, Shiqie, Wu, Yanqi, Dao, Zhixue, Li, Daxu, Zhang, Changbing, Zhang, Yu, You, Minghong, Yang, Fuyu, Zhang, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840007/
https://www.ncbi.nlm.nih.gov/pubmed/24282538
http://dx.doi.org/10.1371/journal.pone.0080388
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author Zhang, Jianbo
Yan, Jiajun
Zhang, Yunwei
Ma, Xiao
Bai, Shiqie
Wu, Yanqi
Dao, Zhixue
Li, Daxu
Zhang, Changbing
Zhang, Yu
You, Minghong
Yang, Fuyu
Zhang, Jin
author_facet Zhang, Jianbo
Yan, Jiajun
Zhang, Yunwei
Ma, Xiao
Bai, Shiqie
Wu, Yanqi
Dao, Zhixue
Li, Daxu
Zhang, Changbing
Zhang, Yu
You, Minghong
Yang, Fuyu
Zhang, Jin
author_sort Zhang, Jianbo
collection PubMed
description BACKGROUND: E. arundinaceus (Retz.) Jeswiet is a warm-season, tall-growing perennial species native to much southern portion in China. The grass has been extensively used in sugarcane breeding and is recently targeted as a bioenergy feedstock crop. However, information on the genetic structure of the Chinese wild germplasm is limited. Knowledge of genetic variation within and among populations is essential for breeding new cultivars in the species. The major objective of this study was to quantify the magnitude of genetic variation among and within natural populations in China. METHODOLOGY/PRINCIPAL FINDINGS: In this experiment, we analyzed genetic variation of 164 individuals of 18 populations collected from natural habitats in six Chinese provinces using 20 sequence-related amplified polymorphism (SRAP) primer pairs generating 277 polymorphic bands. Among and within the populations, the percentage of polymorphic bands (PPB) was 80.00% and 27.07%, genetic diversity (H(E)) was 0.245 and 0.099, effective number of alleles (N(E)) was 1.350 and 1.170, and Shannon's information index (I) was 0.340 and 0.147, respectively. The populations were clustered into six groups exhibiting a high level of genetic differentiation, which was highly associated with geographic origins of respective germplasm populations, but was not significantly associated with geographic distances between the populations. CONCLUSIONS/SIGNIFICANCE: This is the first report indicating that large genetic variation exists in the Chinese E. arundinaceus germplasm based on the SRAP molecular marker analysis of native populations. The genetic structure of populations in the species has been substantially affected by geographic landforms and environments. The diverse collection will be highly valuable in genetic improvement in the species per se and likely in sugarcane.
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spelling pubmed-38400072013-11-26 Molecular Insights of Genetic Variation in Erianthus arundinaceus Populations Native to China Zhang, Jianbo Yan, Jiajun Zhang, Yunwei Ma, Xiao Bai, Shiqie Wu, Yanqi Dao, Zhixue Li, Daxu Zhang, Changbing Zhang, Yu You, Minghong Yang, Fuyu Zhang, Jin PLoS One Research Article BACKGROUND: E. arundinaceus (Retz.) Jeswiet is a warm-season, tall-growing perennial species native to much southern portion in China. The grass has been extensively used in sugarcane breeding and is recently targeted as a bioenergy feedstock crop. However, information on the genetic structure of the Chinese wild germplasm is limited. Knowledge of genetic variation within and among populations is essential for breeding new cultivars in the species. The major objective of this study was to quantify the magnitude of genetic variation among and within natural populations in China. METHODOLOGY/PRINCIPAL FINDINGS: In this experiment, we analyzed genetic variation of 164 individuals of 18 populations collected from natural habitats in six Chinese provinces using 20 sequence-related amplified polymorphism (SRAP) primer pairs generating 277 polymorphic bands. Among and within the populations, the percentage of polymorphic bands (PPB) was 80.00% and 27.07%, genetic diversity (H(E)) was 0.245 and 0.099, effective number of alleles (N(E)) was 1.350 and 1.170, and Shannon's information index (I) was 0.340 and 0.147, respectively. The populations were clustered into six groups exhibiting a high level of genetic differentiation, which was highly associated with geographic origins of respective germplasm populations, but was not significantly associated with geographic distances between the populations. CONCLUSIONS/SIGNIFICANCE: This is the first report indicating that large genetic variation exists in the Chinese E. arundinaceus germplasm based on the SRAP molecular marker analysis of native populations. The genetic structure of populations in the species has been substantially affected by geographic landforms and environments. The diverse collection will be highly valuable in genetic improvement in the species per se and likely in sugarcane. Public Library of Science 2013-11-25 /pmc/articles/PMC3840007/ /pubmed/24282538 http://dx.doi.org/10.1371/journal.pone.0080388 Text en © 2013 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Jianbo
Yan, Jiajun
Zhang, Yunwei
Ma, Xiao
Bai, Shiqie
Wu, Yanqi
Dao, Zhixue
Li, Daxu
Zhang, Changbing
Zhang, Yu
You, Minghong
Yang, Fuyu
Zhang, Jin
Molecular Insights of Genetic Variation in Erianthus arundinaceus Populations Native to China
title Molecular Insights of Genetic Variation in Erianthus arundinaceus Populations Native to China
title_full Molecular Insights of Genetic Variation in Erianthus arundinaceus Populations Native to China
title_fullStr Molecular Insights of Genetic Variation in Erianthus arundinaceus Populations Native to China
title_full_unstemmed Molecular Insights of Genetic Variation in Erianthus arundinaceus Populations Native to China
title_short Molecular Insights of Genetic Variation in Erianthus arundinaceus Populations Native to China
title_sort molecular insights of genetic variation in erianthus arundinaceus populations native to china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840007/
https://www.ncbi.nlm.nih.gov/pubmed/24282538
http://dx.doi.org/10.1371/journal.pone.0080388
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