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Anti-inflammatory effect of a retrovirus-derived immunosuppressive peptide in mouse models
BACKGROUND: Short dimeric or mulitmeric peptides derived from a highly conserved stretch of amino acids from gammaretroviral envelope proteins has been found to have immunosuppressive properties in vitro. Here we test the hypothesis that such immunosuppressive peptides may serve as immunomodulatory...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840592/ https://www.ncbi.nlm.nih.gov/pubmed/24245569 http://dx.doi.org/10.1186/1471-2172-14-51 |
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author | Tolstrup, Martin Johansen, Claus Toft, Lars Pedersen, Finn S Funding, Anne Bahrami, Shervin Iversen, Lars Østergaard, Lars Duch, Mogens |
author_facet | Tolstrup, Martin Johansen, Claus Toft, Lars Pedersen, Finn S Funding, Anne Bahrami, Shervin Iversen, Lars Østergaard, Lars Duch, Mogens |
author_sort | Tolstrup, Martin |
collection | PubMed |
description | BACKGROUND: Short dimeric or mulitmeric peptides derived from a highly conserved stretch of amino acids from gammaretroviral envelope proteins has been found to have immunosuppressive properties in vitro. Here we test the hypothesis that such immunosuppressive peptides may serve as immunomodulatory reagents for treatment of inflammatory disorders. RESULTS: The anti-inflammatory effect of a synthetic retrovirus-derived immunosuppressive peptide of 17 amino acids was tested in two murine skin inflammation models, a TPA-induced acute toxic contact eczema model and an oxazolone-induced allergic contact dermatitis. Overall, mice (n = 24) treated with a topically applied cream containing the dimeric immunosuppressive peptide exhibited a reduction of 28.8% in ear thickness (range 20.1-42.5), whereas the application of a scrambled peptide dimer or a monomer of the immunosuppressive peptide remained without effect (p = 0.028). Furthermore, ear biopsies from mice treated with the dimeric immunosuppressive peptide showed a significant reduction in mRNA of the pro-inflammatory cytokines TNF-α, IL-17C, and IL-6 as well as the chemokine CXCL2 compared to mice treated with control peptides. CONCLUSION: Using two murine skin inflammation models, we show that an immunosuppressive retroviral peptide is capable of reducing inflammatory disorders. The results indicate that virus-derived immunosuppressive peptides capable of down-regulating several proinflammatory cytokines may represent a novel class of drugs for the treatment of excess inflammation. |
format | Online Article Text |
id | pubmed-3840592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38405922013-11-27 Anti-inflammatory effect of a retrovirus-derived immunosuppressive peptide in mouse models Tolstrup, Martin Johansen, Claus Toft, Lars Pedersen, Finn S Funding, Anne Bahrami, Shervin Iversen, Lars Østergaard, Lars Duch, Mogens BMC Immunol Research Article BACKGROUND: Short dimeric or mulitmeric peptides derived from a highly conserved stretch of amino acids from gammaretroviral envelope proteins has been found to have immunosuppressive properties in vitro. Here we test the hypothesis that such immunosuppressive peptides may serve as immunomodulatory reagents for treatment of inflammatory disorders. RESULTS: The anti-inflammatory effect of a synthetic retrovirus-derived immunosuppressive peptide of 17 amino acids was tested in two murine skin inflammation models, a TPA-induced acute toxic contact eczema model and an oxazolone-induced allergic contact dermatitis. Overall, mice (n = 24) treated with a topically applied cream containing the dimeric immunosuppressive peptide exhibited a reduction of 28.8% in ear thickness (range 20.1-42.5), whereas the application of a scrambled peptide dimer or a monomer of the immunosuppressive peptide remained without effect (p = 0.028). Furthermore, ear biopsies from mice treated with the dimeric immunosuppressive peptide showed a significant reduction in mRNA of the pro-inflammatory cytokines TNF-α, IL-17C, and IL-6 as well as the chemokine CXCL2 compared to mice treated with control peptides. CONCLUSION: Using two murine skin inflammation models, we show that an immunosuppressive retroviral peptide is capable of reducing inflammatory disorders. The results indicate that virus-derived immunosuppressive peptides capable of down-regulating several proinflammatory cytokines may represent a novel class of drugs for the treatment of excess inflammation. BioMed Central 2013-11-18 /pmc/articles/PMC3840592/ /pubmed/24245569 http://dx.doi.org/10.1186/1471-2172-14-51 Text en Copyright © 2013 Tolstrup et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Tolstrup, Martin Johansen, Claus Toft, Lars Pedersen, Finn S Funding, Anne Bahrami, Shervin Iversen, Lars Østergaard, Lars Duch, Mogens Anti-inflammatory effect of a retrovirus-derived immunosuppressive peptide in mouse models |
title | Anti-inflammatory effect of a retrovirus-derived immunosuppressive peptide in mouse models |
title_full | Anti-inflammatory effect of a retrovirus-derived immunosuppressive peptide in mouse models |
title_fullStr | Anti-inflammatory effect of a retrovirus-derived immunosuppressive peptide in mouse models |
title_full_unstemmed | Anti-inflammatory effect of a retrovirus-derived immunosuppressive peptide in mouse models |
title_short | Anti-inflammatory effect of a retrovirus-derived immunosuppressive peptide in mouse models |
title_sort | anti-inflammatory effect of a retrovirus-derived immunosuppressive peptide in mouse models |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840592/ https://www.ncbi.nlm.nih.gov/pubmed/24245569 http://dx.doi.org/10.1186/1471-2172-14-51 |
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