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The capability of endophytic fungi for production of hemicellulases and related enzymes
BACKGROUND: There is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry is the bagasse, a rich li...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840621/ https://www.ncbi.nlm.nih.gov/pubmed/24175970 http://dx.doi.org/10.1186/1472-6750-13-94 |
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author | Robl, Diogo Delabona, Priscila da Silva Mergel, Carla Montanari Rojas, Juan Diego Costa, Patrícia dos Santos Pimentel, Ida Chapaval Vicente, Vania Aparecida da Cruz Pradella, José Geraldo Padilla, Gabriel |
author_facet | Robl, Diogo Delabona, Priscila da Silva Mergel, Carla Montanari Rojas, Juan Diego Costa, Patrícia dos Santos Pimentel, Ida Chapaval Vicente, Vania Aparecida da Cruz Pradella, José Geraldo Padilla, Gabriel |
author_sort | Robl, Diogo |
collection | PubMed |
description | BACKGROUND: There is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw material uses for the production of ethanol second generation (2G). New cellulolytic and hemicellulytic enzymes are needed, in order to optimize the degradation of bagasse and production of ethanol 2G. RESULTS: The ability to produce hemicellulases and related enzymes, suitable for lignocellulosic biomass deconstruction, was explored using 110 endophytic fungi and 9 fungi isolated from spoiled books in Brazil. Two initial selections were performed, one employing the esculin gel diffusion assay, and the other by culturing on agar plate media with beechwood xylan and liquor from the hydrothermal pretreatment of sugar cane bagasse. A total of 56 isolates were then grown at 29°C on steam-exploded delignified sugar cane bagasse (DEB) plus soybean bran (SB) (3:1), with measurement of the xylanase, pectinase, β-glucosidase, CMCase, and FPase activities. Twelve strains were selected, and their enzyme extracts were assessed using different substrates. Finally, the best six strains were grown under xylan and pectin, and several glycohydrolases activities were also assessed. These strains were identified morphologically and by sequencing the internal transcribed spacer (ITS) regions and the partial β-tubulin gene (BT2). The best six strains were identified as Aspergillus niger DR02, Trichoderma atroviride DR17 and DR19, Alternaria sp. DR45, Annulohypoxylon stigyum DR47 and Talaromyces wortmannii DR49. These strains produced glycohydrolases with different profiles, and production was highly influenced by the carbon sources in the media. CONCLUSIONS: The selected endophytic fungi Aspergillus niger DR02, Trichoderma atroviride DR17 and DR19, Alternaria sp. DR45, Annulohypoxylon stigyum DR47 and Talaromyces wortmannii DR49 are excellent producers of hydrolytic enzymes to be used as part of blends to decompose sugarcane biomass at industrial level. |
format | Online Article Text |
id | pubmed-3840621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38406212013-11-27 The capability of endophytic fungi for production of hemicellulases and related enzymes Robl, Diogo Delabona, Priscila da Silva Mergel, Carla Montanari Rojas, Juan Diego Costa, Patrícia dos Santos Pimentel, Ida Chapaval Vicente, Vania Aparecida da Cruz Pradella, José Geraldo Padilla, Gabriel BMC Biotechnol Research Article BACKGROUND: There is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw material uses for the production of ethanol second generation (2G). New cellulolytic and hemicellulytic enzymes are needed, in order to optimize the degradation of bagasse and production of ethanol 2G. RESULTS: The ability to produce hemicellulases and related enzymes, suitable for lignocellulosic biomass deconstruction, was explored using 110 endophytic fungi and 9 fungi isolated from spoiled books in Brazil. Two initial selections were performed, one employing the esculin gel diffusion assay, and the other by culturing on agar plate media with beechwood xylan and liquor from the hydrothermal pretreatment of sugar cane bagasse. A total of 56 isolates were then grown at 29°C on steam-exploded delignified sugar cane bagasse (DEB) plus soybean bran (SB) (3:1), with measurement of the xylanase, pectinase, β-glucosidase, CMCase, and FPase activities. Twelve strains were selected, and their enzyme extracts were assessed using different substrates. Finally, the best six strains were grown under xylan and pectin, and several glycohydrolases activities were also assessed. These strains were identified morphologically and by sequencing the internal transcribed spacer (ITS) regions and the partial β-tubulin gene (BT2). The best six strains were identified as Aspergillus niger DR02, Trichoderma atroviride DR17 and DR19, Alternaria sp. DR45, Annulohypoxylon stigyum DR47 and Talaromyces wortmannii DR49. These strains produced glycohydrolases with different profiles, and production was highly influenced by the carbon sources in the media. CONCLUSIONS: The selected endophytic fungi Aspergillus niger DR02, Trichoderma atroviride DR17 and DR19, Alternaria sp. DR45, Annulohypoxylon stigyum DR47 and Talaromyces wortmannii DR49 are excellent producers of hydrolytic enzymes to be used as part of blends to decompose sugarcane biomass at industrial level. BioMed Central 2013-10-31 /pmc/articles/PMC3840621/ /pubmed/24175970 http://dx.doi.org/10.1186/1472-6750-13-94 Text en Copyright © 2013 Robl et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Robl, Diogo Delabona, Priscila da Silva Mergel, Carla Montanari Rojas, Juan Diego Costa, Patrícia dos Santos Pimentel, Ida Chapaval Vicente, Vania Aparecida da Cruz Pradella, José Geraldo Padilla, Gabriel The capability of endophytic fungi for production of hemicellulases and related enzymes |
title | The capability of endophytic fungi for production of hemicellulases and related enzymes |
title_full | The capability of endophytic fungi for production of hemicellulases and related enzymes |
title_fullStr | The capability of endophytic fungi for production of hemicellulases and related enzymes |
title_full_unstemmed | The capability of endophytic fungi for production of hemicellulases and related enzymes |
title_short | The capability of endophytic fungi for production of hemicellulases and related enzymes |
title_sort | capability of endophytic fungi for production of hemicellulases and related enzymes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840621/ https://www.ncbi.nlm.nih.gov/pubmed/24175970 http://dx.doi.org/10.1186/1472-6750-13-94 |
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