Efficient generation of recombinant RNA viruses using targeted recombination-mediated mutagenesis of bacterial artificial chromosomes containing full-length cDNA

BACKGROUND: Infectious cDNA clones are a prerequisite for directed genetic manipulation of RNA viruses. Here, a strategy to facilitate manipulation and rescue of classical swine fever viruses (CSFVs) from full-length cDNAs present within bacterial artificial chromosomes (BACs) is described. This str...

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Detalles Bibliográficos
Autores principales: Rasmussen, Thomas Bruun, Risager, Peter Christian, Fahnøe, Ulrik, Friis, Martin Barfred, Belsham, Graham J, Höper, Dirk, Reimann, Ilona, Beer, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3840674/
https://www.ncbi.nlm.nih.gov/pubmed/24262008
http://dx.doi.org/10.1186/1471-2164-14-819

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