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A novel experimental model of orthopedic trauma with acute kidney injury in obese Zucker rats

Obesity is associated with an increased risk of acute kidney injury (AKI) after blunt traumatic injury in humans. Because limitations exist in studying trauma in human patients, animal models are necessary to elucidate mechanisms of remote organ injury after trauma. We developed a model of severe or...

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Autores principales: Mittwede, Peter N, Xiang, Lusha, Lu, Silu, Clemmer, John S, Hester, Robert L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841033/
https://www.ncbi.nlm.nih.gov/pubmed/24303169
http://dx.doi.org/10.1002/phy2.97
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author Mittwede, Peter N
Xiang, Lusha
Lu, Silu
Clemmer, John S
Hester, Robert L
author_facet Mittwede, Peter N
Xiang, Lusha
Lu, Silu
Clemmer, John S
Hester, Robert L
author_sort Mittwede, Peter N
collection PubMed
description Obesity is associated with an increased risk of acute kidney injury (AKI) after blunt traumatic injury in humans. Because limitations exist in studying trauma in human patients, animal models are necessary to elucidate mechanisms of remote organ injury after trauma. We developed a model of severe orthopedic trauma in lean (LZ) and obese (OZ) Zucker rats, in which OZ develop greater kidney dysfunction after trauma than LZ. Orthopedic trauma was inflicted via bilateral hindlimb soft tissue injury, fibula fracture, and injection of homogenized bone components. Mean arterial pressure (MAP) and heart rate (HR) were measured for 6 h after trauma, and again at 24 h after trauma. Urine was collected for 24 h before and after trauma to measure urine albumin excretion. Glomerular filtration rate (GFR), renal plasma flow (RPF), plasma interleukin-6 (IL-6), and renal macrophage infiltration (ED-1 [CD68 Antibody] immunostaining) were measured in animals with and without trauma. MAP and HR were similar between LZ and OZ throughout the study, with the exception that OZ had a 18 mmHg lower pressure 24 h posttrauma. GFR and RPF were decreased significantly (∼50%), while urine albumin excretion, plasma IL-6, and renal ED-1-positive cells were increased in OZ 24 h after trauma compared to both OZ without trauma and LZ after trauma. In conclusion, these data are consistent with studies in humans that show that AKI develops more frequently in obese than in lean individuals. This model will be an important experimental tool to better understand the underlying mechanisms of poor outcomes after trauma in obese patients.
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spelling pubmed-38410332013-12-03 A novel experimental model of orthopedic trauma with acute kidney injury in obese Zucker rats Mittwede, Peter N Xiang, Lusha Lu, Silu Clemmer, John S Hester, Robert L Physiol Rep Original Research Obesity is associated with an increased risk of acute kidney injury (AKI) after blunt traumatic injury in humans. Because limitations exist in studying trauma in human patients, animal models are necessary to elucidate mechanisms of remote organ injury after trauma. We developed a model of severe orthopedic trauma in lean (LZ) and obese (OZ) Zucker rats, in which OZ develop greater kidney dysfunction after trauma than LZ. Orthopedic trauma was inflicted via bilateral hindlimb soft tissue injury, fibula fracture, and injection of homogenized bone components. Mean arterial pressure (MAP) and heart rate (HR) were measured for 6 h after trauma, and again at 24 h after trauma. Urine was collected for 24 h before and after trauma to measure urine albumin excretion. Glomerular filtration rate (GFR), renal plasma flow (RPF), plasma interleukin-6 (IL-6), and renal macrophage infiltration (ED-1 [CD68 Antibody] immunostaining) were measured in animals with and without trauma. MAP and HR were similar between LZ and OZ throughout the study, with the exception that OZ had a 18 mmHg lower pressure 24 h posttrauma. GFR and RPF were decreased significantly (∼50%), while urine albumin excretion, plasma IL-6, and renal ED-1-positive cells were increased in OZ 24 h after trauma compared to both OZ without trauma and LZ after trauma. In conclusion, these data are consistent with studies in humans that show that AKI develops more frequently in obese than in lean individuals. This model will be an important experimental tool to better understand the underlying mechanisms of poor outcomes after trauma in obese patients. Blackwell Publishing Ltd 2013-10 2013-10-02 /pmc/articles/PMC3841033/ /pubmed/24303169 http://dx.doi.org/10.1002/phy2.97 Text en © 2013 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Original Research
Mittwede, Peter N
Xiang, Lusha
Lu, Silu
Clemmer, John S
Hester, Robert L
A novel experimental model of orthopedic trauma with acute kidney injury in obese Zucker rats
title A novel experimental model of orthopedic trauma with acute kidney injury in obese Zucker rats
title_full A novel experimental model of orthopedic trauma with acute kidney injury in obese Zucker rats
title_fullStr A novel experimental model of orthopedic trauma with acute kidney injury in obese Zucker rats
title_full_unstemmed A novel experimental model of orthopedic trauma with acute kidney injury in obese Zucker rats
title_short A novel experimental model of orthopedic trauma with acute kidney injury in obese Zucker rats
title_sort novel experimental model of orthopedic trauma with acute kidney injury in obese zucker rats
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841033/
https://www.ncbi.nlm.nih.gov/pubmed/24303169
http://dx.doi.org/10.1002/phy2.97
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