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Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region

Vibrio cholerae is a natural inhabitant of many aquatic environments in the world. Biotypes harboring similar virulence-related gene clusters are the causative agents of epidemic cholera, but the majority of strains are harmless to humans. Since 1971, environmental surveillance for potentially patho...

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Autores principales: Morais, Lena Líllian Canto de Sá, Garza, Daniel Rios, Loureiro, Edvaldo Carlos Brito, Vale, Elivam Rodrigues, Santos, Denise Suéllem Amorim de Sousa, Corrêa, Vanessa Cavaleiro, Sousa, Nayara Rufino, Gurjão, Tereza Cristina Monteiro, Santos, Elisabeth Conceição de Oliveira, Vieira, Verônica Viana, da Fonseca, Erica Lourenço, Vicente, Ana Carolina Paulo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841125/
https://www.ncbi.nlm.nih.gov/pubmed/24303045
http://dx.doi.org/10.1371/journal.pone.0081372
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author Morais, Lena Líllian Canto de Sá
Garza, Daniel Rios
Loureiro, Edvaldo Carlos Brito
Vale, Elivam Rodrigues
Santos, Denise Suéllem Amorim de Sousa
Corrêa, Vanessa Cavaleiro
Sousa, Nayara Rufino
Gurjão, Tereza Cristina Monteiro
Santos, Elisabeth Conceição de Oliveira
Vieira, Verônica Viana
da Fonseca, Erica Lourenço
Vicente, Ana Carolina Paulo
author_facet Morais, Lena Líllian Canto de Sá
Garza, Daniel Rios
Loureiro, Edvaldo Carlos Brito
Vale, Elivam Rodrigues
Santos, Denise Suéllem Amorim de Sousa
Corrêa, Vanessa Cavaleiro
Sousa, Nayara Rufino
Gurjão, Tereza Cristina Monteiro
Santos, Elisabeth Conceição de Oliveira
Vieira, Verônica Viana
da Fonseca, Erica Lourenço
Vicente, Ana Carolina Paulo
author_sort Morais, Lena Líllian Canto de Sá
collection PubMed
description Vibrio cholerae is a natural inhabitant of many aquatic environments in the world. Biotypes harboring similar virulence-related gene clusters are the causative agents of epidemic cholera, but the majority of strains are harmless to humans. Since 1971, environmental surveillance for potentially pathogenic V. cholerae has resulted in the isolation of many strains from the Brazilian Amazon aquatic ecosystem. Most of these strains are from the non-O1/non-O139 serogroups (NAGs), but toxigenic O1 strains were isolated during the Latin America cholera epidemic in the region (1991-1996). A collection of environmental V. cholerae strains from the Brazilian Amazon belonging to pre-epidemic (1977-1990), epidemic (1991-1996), and post-epidemic (1996-2007) periods in the region, was analyzed. The presence of genes related to virulence within the species and the genetic relationship among the strains were studied. These variables and the information available concerning the strains were used to build a Bayesian multivariate dependency model to distinguish the importance of each variable in determining the others. Some genes related to the epidemic strains were found in environmental NAGs during and after the epidemic. Significant diversity among the virulence-related gene content was observed among O1 strains isolated from the environment during the epidemic period, but not from clinical isolates, which were analyzed as controls. Despite this diversity, these strains exhibited similar PFGE profiles. PFGE profiles were significant while separating potentially epidemic clones from indigenous strains. No significant correlation with isolation source, place or period was observed. The presence of the WASA-1 prophage significantly correlated with serogroups, PFGE profiles, and the presence of virulence-related genes. This study provides a broad characterization of the environmental V. cholerae population from the Amazon, and also highlights the importance of identifying precisely defined genetic markers such as the WASA-1 prophage for the surveillance of cholera.
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spelling pubmed-38411252013-12-03 Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region Morais, Lena Líllian Canto de Sá Garza, Daniel Rios Loureiro, Edvaldo Carlos Brito Vale, Elivam Rodrigues Santos, Denise Suéllem Amorim de Sousa Corrêa, Vanessa Cavaleiro Sousa, Nayara Rufino Gurjão, Tereza Cristina Monteiro Santos, Elisabeth Conceição de Oliveira Vieira, Verônica Viana da Fonseca, Erica Lourenço Vicente, Ana Carolina Paulo PLoS One Research Article Vibrio cholerae is a natural inhabitant of many aquatic environments in the world. Biotypes harboring similar virulence-related gene clusters are the causative agents of epidemic cholera, but the majority of strains are harmless to humans. Since 1971, environmental surveillance for potentially pathogenic V. cholerae has resulted in the isolation of many strains from the Brazilian Amazon aquatic ecosystem. Most of these strains are from the non-O1/non-O139 serogroups (NAGs), but toxigenic O1 strains were isolated during the Latin America cholera epidemic in the region (1991-1996). A collection of environmental V. cholerae strains from the Brazilian Amazon belonging to pre-epidemic (1977-1990), epidemic (1991-1996), and post-epidemic (1996-2007) periods in the region, was analyzed. The presence of genes related to virulence within the species and the genetic relationship among the strains were studied. These variables and the information available concerning the strains were used to build a Bayesian multivariate dependency model to distinguish the importance of each variable in determining the others. Some genes related to the epidemic strains were found in environmental NAGs during and after the epidemic. Significant diversity among the virulence-related gene content was observed among O1 strains isolated from the environment during the epidemic period, but not from clinical isolates, which were analyzed as controls. Despite this diversity, these strains exhibited similar PFGE profiles. PFGE profiles were significant while separating potentially epidemic clones from indigenous strains. No significant correlation with isolation source, place or period was observed. The presence of the WASA-1 prophage significantly correlated with serogroups, PFGE profiles, and the presence of virulence-related genes. This study provides a broad characterization of the environmental V. cholerae population from the Amazon, and also highlights the importance of identifying precisely defined genetic markers such as the WASA-1 prophage for the surveillance of cholera. Public Library of Science 2013-11-26 /pmc/articles/PMC3841125/ /pubmed/24303045 http://dx.doi.org/10.1371/journal.pone.0081372 Text en © 2013 Morais et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Morais, Lena Líllian Canto de Sá
Garza, Daniel Rios
Loureiro, Edvaldo Carlos Brito
Vale, Elivam Rodrigues
Santos, Denise Suéllem Amorim de Sousa
Corrêa, Vanessa Cavaleiro
Sousa, Nayara Rufino
Gurjão, Tereza Cristina Monteiro
Santos, Elisabeth Conceição de Oliveira
Vieira, Verônica Viana
da Fonseca, Erica Lourenço
Vicente, Ana Carolina Paulo
Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region
title Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region
title_full Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region
title_fullStr Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region
title_full_unstemmed Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region
title_short Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region
title_sort population and genetic study of vibrio cholerae from the amazon environment confirms that the wasa-1 prophage is the main marker of the epidemic strain that circulated in the region
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841125/
https://www.ncbi.nlm.nih.gov/pubmed/24303045
http://dx.doi.org/10.1371/journal.pone.0081372
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