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The Gastric CB1 Receptor Modulates Ghrelin Production through the mTOR Pathway to Regulate Food Intake

Over the years, the knowledge regarding the relevance of the cannabinoid system to the regulation of metabolism has grown steadily. A central interaction between the cannabinoid system and ghrelin has been suggested to regulate food intake. Although the stomach is the main source of ghrelin and CB1...

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Autores principales: Senin, Lucia L., Al-Massadi, Omar, Folgueira, Cintia, Castelao, Cecilia, Pardo, Maria, Barja-Fernandez, Silvia, Roca-Rivada, Arturo, Amil, Maria, Crujeiras, Ana B., Garcia-Caballero, Tomas, Gabellieri, Enrico, Leis, Rosaura, Dieguez, Carlos, Pagotto, Uberto, Casanueva, Felipe F., Seoane, Luisa M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841176/
https://www.ncbi.nlm.nih.gov/pubmed/24303008
http://dx.doi.org/10.1371/journal.pone.0080339
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author Senin, Lucia L.
Al-Massadi, Omar
Folgueira, Cintia
Castelao, Cecilia
Pardo, Maria
Barja-Fernandez, Silvia
Roca-Rivada, Arturo
Amil, Maria
Crujeiras, Ana B.
Garcia-Caballero, Tomas
Gabellieri, Enrico
Leis, Rosaura
Dieguez, Carlos
Pagotto, Uberto
Casanueva, Felipe F.
Seoane, Luisa M.
author_facet Senin, Lucia L.
Al-Massadi, Omar
Folgueira, Cintia
Castelao, Cecilia
Pardo, Maria
Barja-Fernandez, Silvia
Roca-Rivada, Arturo
Amil, Maria
Crujeiras, Ana B.
Garcia-Caballero, Tomas
Gabellieri, Enrico
Leis, Rosaura
Dieguez, Carlos
Pagotto, Uberto
Casanueva, Felipe F.
Seoane, Luisa M.
author_sort Senin, Lucia L.
collection PubMed
description Over the years, the knowledge regarding the relevance of the cannabinoid system to the regulation of metabolism has grown steadily. A central interaction between the cannabinoid system and ghrelin has been suggested to regulate food intake. Although the stomach is the main source of ghrelin and CB1 receptor expression in the stomach has been described, little information is available regarding the possible interaction between the gastric cannabinoid and ghrelin systems in the integrated control of energy homeostasis. The main objective of the present work was to assess the functional interaction between these two systems in terms of food intake using a combination of in vivo and in vitro approaches. The present work demonstrates that the peripheral blockade of the CB1 receptor by rimonabant treatment decreased food intake but only in food-deprived animals. This anorexigenic effect is likely a consequence of decreases in gastric ghrelin secretion induced by the activation of the mTOR/S6K1 intracellular pathway in the stomach following treatment with rimonabant. In support of this supposition, animals in which the mTOR/S6K1 intracellular pathway was blocked by chronic rapamycin treatment, rimonabant had no effect on ghrelin secretion. Vagal communication may also be involved because rimonabant treatment was no longer effective when administered to animals that had undergone surgical vagotomy. In conclusion, to the best of our knowledge, the present work is the first to describe a CB1 receptor-mediated mechanism that influences gastric ghrelin secretion and food intake through the mTOR pathway.
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spelling pubmed-38411762013-12-03 The Gastric CB1 Receptor Modulates Ghrelin Production through the mTOR Pathway to Regulate Food Intake Senin, Lucia L. Al-Massadi, Omar Folgueira, Cintia Castelao, Cecilia Pardo, Maria Barja-Fernandez, Silvia Roca-Rivada, Arturo Amil, Maria Crujeiras, Ana B. Garcia-Caballero, Tomas Gabellieri, Enrico Leis, Rosaura Dieguez, Carlos Pagotto, Uberto Casanueva, Felipe F. Seoane, Luisa M. PLoS One Research Article Over the years, the knowledge regarding the relevance of the cannabinoid system to the regulation of metabolism has grown steadily. A central interaction between the cannabinoid system and ghrelin has been suggested to regulate food intake. Although the stomach is the main source of ghrelin and CB1 receptor expression in the stomach has been described, little information is available regarding the possible interaction between the gastric cannabinoid and ghrelin systems in the integrated control of energy homeostasis. The main objective of the present work was to assess the functional interaction between these two systems in terms of food intake using a combination of in vivo and in vitro approaches. The present work demonstrates that the peripheral blockade of the CB1 receptor by rimonabant treatment decreased food intake but only in food-deprived animals. This anorexigenic effect is likely a consequence of decreases in gastric ghrelin secretion induced by the activation of the mTOR/S6K1 intracellular pathway in the stomach following treatment with rimonabant. In support of this supposition, animals in which the mTOR/S6K1 intracellular pathway was blocked by chronic rapamycin treatment, rimonabant had no effect on ghrelin secretion. Vagal communication may also be involved because rimonabant treatment was no longer effective when administered to animals that had undergone surgical vagotomy. In conclusion, to the best of our knowledge, the present work is the first to describe a CB1 receptor-mediated mechanism that influences gastric ghrelin secretion and food intake through the mTOR pathway. Public Library of Science 2013-11-26 /pmc/articles/PMC3841176/ /pubmed/24303008 http://dx.doi.org/10.1371/journal.pone.0080339 Text en © 2013 Senin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Senin, Lucia L.
Al-Massadi, Omar
Folgueira, Cintia
Castelao, Cecilia
Pardo, Maria
Barja-Fernandez, Silvia
Roca-Rivada, Arturo
Amil, Maria
Crujeiras, Ana B.
Garcia-Caballero, Tomas
Gabellieri, Enrico
Leis, Rosaura
Dieguez, Carlos
Pagotto, Uberto
Casanueva, Felipe F.
Seoane, Luisa M.
The Gastric CB1 Receptor Modulates Ghrelin Production through the mTOR Pathway to Regulate Food Intake
title The Gastric CB1 Receptor Modulates Ghrelin Production through the mTOR Pathway to Regulate Food Intake
title_full The Gastric CB1 Receptor Modulates Ghrelin Production through the mTOR Pathway to Regulate Food Intake
title_fullStr The Gastric CB1 Receptor Modulates Ghrelin Production through the mTOR Pathway to Regulate Food Intake
title_full_unstemmed The Gastric CB1 Receptor Modulates Ghrelin Production through the mTOR Pathway to Regulate Food Intake
title_short The Gastric CB1 Receptor Modulates Ghrelin Production through the mTOR Pathway to Regulate Food Intake
title_sort gastric cb1 receptor modulates ghrelin production through the mtor pathway to regulate food intake
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841176/
https://www.ncbi.nlm.nih.gov/pubmed/24303008
http://dx.doi.org/10.1371/journal.pone.0080339
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