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Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors
The aim of this study was to analyze whether tyrosine phosphorylation in tumoral arteries may modulate their vascular response. To do this, mesenteric arteries supplying blood flow to colorectal tumors or to normal intestine were obtained during surgery and prepared for isometric tension recording i...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842070/ https://www.ncbi.nlm.nih.gov/pubmed/24324963 http://dx.doi.org/10.1155/2013/545983 |
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author | Ferrero, Eduardo Mauricio, María Dolores Granado, Miriam García-Villar, Oscar Aldasoro, Martín Vila, José María Hidalgo, Manuel Ferrero, Jorge Luis Fernández, Nuria Monge, Luis García-Villalón, Ángel Luis |
author_facet | Ferrero, Eduardo Mauricio, María Dolores Granado, Miriam García-Villar, Oscar Aldasoro, Martín Vila, José María Hidalgo, Manuel Ferrero, Jorge Luis Fernández, Nuria Monge, Luis García-Villalón, Ángel Luis |
author_sort | Ferrero, Eduardo |
collection | PubMed |
description | The aim of this study was to analyze whether tyrosine phosphorylation in tumoral arteries may modulate their vascular response. To do this, mesenteric arteries supplying blood flow to colorectal tumors or to normal intestine were obtained during surgery and prepared for isometric tension recording in an organ bath. Increasing tyrosine phosphorylation with the phosphatase inhibitor, sodium orthovanadate produced arterial contraction which was lower in tumoral than in control arteries, whereas it reduced the contraction to noradrenaline in tumoral but not in control arteries and reduced the relaxation to bradykinin in control but not in tumoral arteries. Protein expression of VEGF-A and of the VEGF receptor FLT1 was similar in control and tumoral arteries, but expression of the VEGF receptor KDR was increased in tumoral compared with control arteries. This suggests that tyrosine phosphorylation may produce inhibition of the contraction in tumoral mesenteric arteries, which may increase blood flow to the tumor when tyrosine phosphorylation is increased by stimulation of VEGF receptors. |
format | Online Article Text |
id | pubmed-3842070 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38420702013-12-09 Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors Ferrero, Eduardo Mauricio, María Dolores Granado, Miriam García-Villar, Oscar Aldasoro, Martín Vila, José María Hidalgo, Manuel Ferrero, Jorge Luis Fernández, Nuria Monge, Luis García-Villalón, Ángel Luis Biomed Res Int Research Article The aim of this study was to analyze whether tyrosine phosphorylation in tumoral arteries may modulate their vascular response. To do this, mesenteric arteries supplying blood flow to colorectal tumors or to normal intestine were obtained during surgery and prepared for isometric tension recording in an organ bath. Increasing tyrosine phosphorylation with the phosphatase inhibitor, sodium orthovanadate produced arterial contraction which was lower in tumoral than in control arteries, whereas it reduced the contraction to noradrenaline in tumoral but not in control arteries and reduced the relaxation to bradykinin in control but not in tumoral arteries. Protein expression of VEGF-A and of the VEGF receptor FLT1 was similar in control and tumoral arteries, but expression of the VEGF receptor KDR was increased in tumoral compared with control arteries. This suggests that tyrosine phosphorylation may produce inhibition of the contraction in tumoral mesenteric arteries, which may increase blood flow to the tumor when tyrosine phosphorylation is increased by stimulation of VEGF receptors. Hindawi Publishing Corporation 2013 2013-11-10 /pmc/articles/PMC3842070/ /pubmed/24324963 http://dx.doi.org/10.1155/2013/545983 Text en Copyright © 2013 Eduardo Ferrero et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ferrero, Eduardo Mauricio, María Dolores Granado, Miriam García-Villar, Oscar Aldasoro, Martín Vila, José María Hidalgo, Manuel Ferrero, Jorge Luis Fernández, Nuria Monge, Luis García-Villalón, Ángel Luis Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors |
title | Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors |
title_full | Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors |
title_fullStr | Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors |
title_full_unstemmed | Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors |
title_short | Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors |
title_sort | tyrosine phosphorylation modulates the vascular responses of mesenteric arteries from human colorectal tumors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842070/ https://www.ncbi.nlm.nih.gov/pubmed/24324963 http://dx.doi.org/10.1155/2013/545983 |
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