Cargando…

Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors

The aim of this study was to analyze whether tyrosine phosphorylation in tumoral arteries may modulate their vascular response. To do this, mesenteric arteries supplying blood flow to colorectal tumors or to normal intestine were obtained during surgery and prepared for isometric tension recording i...

Descripción completa

Detalles Bibliográficos
Autores principales: Ferrero, Eduardo, Mauricio, María Dolores, Granado, Miriam, García-Villar, Oscar, Aldasoro, Martín, Vila, José María, Hidalgo, Manuel, Ferrero, Jorge Luis, Fernández, Nuria, Monge, Luis, García-Villalón, Ángel Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842070/
https://www.ncbi.nlm.nih.gov/pubmed/24324963
http://dx.doi.org/10.1155/2013/545983
_version_ 1782292887097049088
author Ferrero, Eduardo
Mauricio, María Dolores
Granado, Miriam
García-Villar, Oscar
Aldasoro, Martín
Vila, José María
Hidalgo, Manuel
Ferrero, Jorge Luis
Fernández, Nuria
Monge, Luis
García-Villalón, Ángel Luis
author_facet Ferrero, Eduardo
Mauricio, María Dolores
Granado, Miriam
García-Villar, Oscar
Aldasoro, Martín
Vila, José María
Hidalgo, Manuel
Ferrero, Jorge Luis
Fernández, Nuria
Monge, Luis
García-Villalón, Ángel Luis
author_sort Ferrero, Eduardo
collection PubMed
description The aim of this study was to analyze whether tyrosine phosphorylation in tumoral arteries may modulate their vascular response. To do this, mesenteric arteries supplying blood flow to colorectal tumors or to normal intestine were obtained during surgery and prepared for isometric tension recording in an organ bath. Increasing tyrosine phosphorylation with the phosphatase inhibitor, sodium orthovanadate produced arterial contraction which was lower in tumoral than in control arteries, whereas it reduced the contraction to noradrenaline in tumoral but not in control arteries and reduced the relaxation to bradykinin in control but not in tumoral arteries. Protein expression of VEGF-A and of the VEGF receptor FLT1 was similar in control and tumoral arteries, but expression of the VEGF receptor KDR was increased in tumoral compared with control arteries. This suggests that tyrosine phosphorylation may produce inhibition of the contraction in tumoral mesenteric arteries, which may increase blood flow to the tumor when tyrosine phosphorylation is increased by stimulation of VEGF receptors.
format Online
Article
Text
id pubmed-3842070
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-38420702013-12-09 Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors Ferrero, Eduardo Mauricio, María Dolores Granado, Miriam García-Villar, Oscar Aldasoro, Martín Vila, José María Hidalgo, Manuel Ferrero, Jorge Luis Fernández, Nuria Monge, Luis García-Villalón, Ángel Luis Biomed Res Int Research Article The aim of this study was to analyze whether tyrosine phosphorylation in tumoral arteries may modulate their vascular response. To do this, mesenteric arteries supplying blood flow to colorectal tumors or to normal intestine were obtained during surgery and prepared for isometric tension recording in an organ bath. Increasing tyrosine phosphorylation with the phosphatase inhibitor, sodium orthovanadate produced arterial contraction which was lower in tumoral than in control arteries, whereas it reduced the contraction to noradrenaline in tumoral but not in control arteries and reduced the relaxation to bradykinin in control but not in tumoral arteries. Protein expression of VEGF-A and of the VEGF receptor FLT1 was similar in control and tumoral arteries, but expression of the VEGF receptor KDR was increased in tumoral compared with control arteries. This suggests that tyrosine phosphorylation may produce inhibition of the contraction in tumoral mesenteric arteries, which may increase blood flow to the tumor when tyrosine phosphorylation is increased by stimulation of VEGF receptors. Hindawi Publishing Corporation 2013 2013-11-10 /pmc/articles/PMC3842070/ /pubmed/24324963 http://dx.doi.org/10.1155/2013/545983 Text en Copyright © 2013 Eduardo Ferrero et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ferrero, Eduardo
Mauricio, María Dolores
Granado, Miriam
García-Villar, Oscar
Aldasoro, Martín
Vila, José María
Hidalgo, Manuel
Ferrero, Jorge Luis
Fernández, Nuria
Monge, Luis
García-Villalón, Ángel Luis
Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors
title Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors
title_full Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors
title_fullStr Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors
title_full_unstemmed Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors
title_short Tyrosine Phosphorylation Modulates the Vascular Responses of Mesenteric Arteries from Human Colorectal Tumors
title_sort tyrosine phosphorylation modulates the vascular responses of mesenteric arteries from human colorectal tumors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842070/
https://www.ncbi.nlm.nih.gov/pubmed/24324963
http://dx.doi.org/10.1155/2013/545983
work_keys_str_mv AT ferreroeduardo tyrosinephosphorylationmodulatesthevascularresponsesofmesentericarteriesfromhumancolorectaltumors
AT mauriciomariadolores tyrosinephosphorylationmodulatesthevascularresponsesofmesentericarteriesfromhumancolorectaltumors
AT granadomiriam tyrosinephosphorylationmodulatesthevascularresponsesofmesentericarteriesfromhumancolorectaltumors
AT garciavillaroscar tyrosinephosphorylationmodulatesthevascularresponsesofmesentericarteriesfromhumancolorectaltumors
AT aldasoromartin tyrosinephosphorylationmodulatesthevascularresponsesofmesentericarteriesfromhumancolorectaltumors
AT vilajosemaria tyrosinephosphorylationmodulatesthevascularresponsesofmesentericarteriesfromhumancolorectaltumors
AT hidalgomanuel tyrosinephosphorylationmodulatesthevascularresponsesofmesentericarteriesfromhumancolorectaltumors
AT ferrerojorgeluis tyrosinephosphorylationmodulatesthevascularresponsesofmesentericarteriesfromhumancolorectaltumors
AT fernandeznuria tyrosinephosphorylationmodulatesthevascularresponsesofmesentericarteriesfromhumancolorectaltumors
AT mongeluis tyrosinephosphorylationmodulatesthevascularresponsesofmesentericarteriesfromhumancolorectaltumors
AT garciavillalonangelluis tyrosinephosphorylationmodulatesthevascularresponsesofmesentericarteriesfromhumancolorectaltumors