Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA

CRISPR/Cas mediated genome editing has been successfully demonstrated in mammalian cells and further applications for generating mutant mice were reported by injecting humanized Cas9 (hCas) mRNA and single guide RNA into fertilized eggs. Here we inject the circular plasmids expressing hCas9 and sgRN...

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Detalles Bibliográficos
Autores principales: Mashiko, Daisuke, Fujihara, Yoshitaka, Satouh, Yuhkoh, Miyata, Haruhiko, Isotani, Ayako, Ikawa, Masahito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842082/
https://www.ncbi.nlm.nih.gov/pubmed/24284873
http://dx.doi.org/10.1038/srep03355

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842082/
https://www.ncbi.nlm.nih.gov/pubmed/24284873
http://dx.doi.org/10.1038/srep03355

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